Islet Cannabinoid Receptors Cellular Distribution and Biological Function

Autores
Gagliardino, Juan Jose; Flores, Luis Emilio; Alzugaray, Maria Eugenia; Cubilla, Marisa Angelica; Raschia, Maria A.; del Zotto, Hector Herminio; Román, Carolina Lisi; Suburo, Angela Maria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To determine the cellular distribution of islet cannabinoid receptors (CBs) and their involvement in the development of metabolic and hormonal changes in rats fed a fructose-rich diet (F). Methods: In normal rat islets, we determined CBs (immunofluorescence and RT-PCR) and glucose-stimulated insulin secretion (GSIS) of isolated islets incubated with the CB1 antagonist rimonabant (R) and/or different CBs agonists. In 3-week F-fed rats, we determined the in vivo effect of R upon serum glucose, triglyceride and insulin levels, HOMA-IR, GSIS and CBs and IRS gene expression levels (real-time PCR). Results: CBs appeared exclusively in islet α-cells. Whereas different CBs agonists enhanced GSIS in normal rat islets, R did not affect it. F rats had higher serum triglyceride and insulin levels and HOMA-IR than control rats; these alterations were prevented by R coadministration. Although R did not correct the increased GSIS observed in F islets, it modulated CBs and IRS gene expression. Conclusion: Islet CBs would exert an important modulatory role in metabolic homeostasis. Administration of R and F affected islet CBs expression and prevented the development of Finduced metabolic impairment. Selective islet CB1 blockers could be useful to prevent/treat the alterations induced by the intake of unbalanced/unhealthy diets.
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Flores, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina
Fil: Alzugaray, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata; Argentina
Fil: Cubilla, Marisa Angelica. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina
Fil: Raschia, Maria A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Román, Carolina Lisi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
Materia
Cannabinoid Receptors,
Pancreatic Islets,
Metabolic Syndrome,
A-Cell Function,
Dyslipidemia,
Metabolic Homeostasis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3405
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Islet Cannabinoid Receptors Cellular Distribution and Biological FunctionGagliardino, Juan JoseFlores, Luis EmilioAlzugaray, Maria EugeniaCubilla, Marisa AngelicaRaschia, Maria A.del Zotto, Hector HerminioRomán, Carolina LisiSuburo, Angela MariaCannabinoid Receptors,Pancreatic Islets,Metabolic Syndrome,A-Cell Function,Dyslipidemia,Metabolic Homeostasishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objectives: To determine the cellular distribution of islet cannabinoid receptors (CBs) and their involvement in the development of metabolic and hormonal changes in rats fed a fructose-rich diet (F). Methods: In normal rat islets, we determined CBs (immunofluorescence and RT-PCR) and glucose-stimulated insulin secretion (GSIS) of isolated islets incubated with the CB1 antagonist rimonabant (R) and/or different CBs agonists. In 3-week F-fed rats, we determined the in vivo effect of R upon serum glucose, triglyceride and insulin levels, HOMA-IR, GSIS and CBs and IRS gene expression levels (real-time PCR). Results: CBs appeared exclusively in islet α-cells. Whereas different CBs agonists enhanced GSIS in normal rat islets, R did not affect it. F rats had higher serum triglyceride and insulin levels and HOMA-IR than control rats; these alterations were prevented by R coadministration. Although R did not correct the increased GSIS observed in F islets, it modulated CBs and IRS gene expression. Conclusion: Islet CBs would exert an important modulatory role in metabolic homeostasis. Administration of R and F affected islet CBs expression and prevented the development of Finduced metabolic impairment. Selective islet CB1 blockers could be useful to prevent/treat the alterations induced by the intake of unbalanced/unhealthy diets.Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; ArgentinaFil: Flores, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); ArgentinaFil: Alzugaray, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata; ArgentinaFil: Cubilla, Marisa Angelica. Universidad Austral. Facultad de Ciencias Biomédicas; ArgentinaFil: Raschia, Maria A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; ArgentinaFil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; ArgentinaFil: Román, Carolina Lisi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); ArgentinaFil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomedicas; ArgentinaLippincott, Williams2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/mswordapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3405Gagliardino, Juan Jose; Flores, Luis Emilio; Alzugaray, Maria Eugenia; Cubilla, Marisa Angelica; Raschia, Maria A.; et al.; Islet Cannabinoid Receptors Cellular Distribution and Biological Function; Lippincott, Williams; Pancreas; 42; 10-2013; 1085-10920885-3177enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/24005231info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:44Zoai:ri.conicet.gov.ar:11336/3405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:44.53CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Islet Cannabinoid Receptors Cellular Distribution and Biological Function
title Islet Cannabinoid Receptors Cellular Distribution and Biological Function
spellingShingle Islet Cannabinoid Receptors Cellular Distribution and Biological Function
Gagliardino, Juan Jose
Cannabinoid Receptors,
Pancreatic Islets,
Metabolic Syndrome,
A-Cell Function,
Dyslipidemia,
Metabolic Homeostasis
title_short Islet Cannabinoid Receptors Cellular Distribution and Biological Function
title_full Islet Cannabinoid Receptors Cellular Distribution and Biological Function
title_fullStr Islet Cannabinoid Receptors Cellular Distribution and Biological Function
title_full_unstemmed Islet Cannabinoid Receptors Cellular Distribution and Biological Function
title_sort Islet Cannabinoid Receptors Cellular Distribution and Biological Function
dc.creator.none.fl_str_mv Gagliardino, Juan Jose
Flores, Luis Emilio
Alzugaray, Maria Eugenia
Cubilla, Marisa Angelica
Raschia, Maria A.
del Zotto, Hector Herminio
Román, Carolina Lisi
Suburo, Angela Maria
author Gagliardino, Juan Jose
author_facet Gagliardino, Juan Jose
Flores, Luis Emilio
Alzugaray, Maria Eugenia
Cubilla, Marisa Angelica
Raschia, Maria A.
del Zotto, Hector Herminio
Román, Carolina Lisi
Suburo, Angela Maria
author_role author
author2 Flores, Luis Emilio
Alzugaray, Maria Eugenia
Cubilla, Marisa Angelica
Raschia, Maria A.
del Zotto, Hector Herminio
Román, Carolina Lisi
Suburo, Angela Maria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cannabinoid Receptors,
Pancreatic Islets,
Metabolic Syndrome,
A-Cell Function,
Dyslipidemia,
Metabolic Homeostasis
topic Cannabinoid Receptors,
Pancreatic Islets,
Metabolic Syndrome,
A-Cell Function,
Dyslipidemia,
Metabolic Homeostasis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: To determine the cellular distribution of islet cannabinoid receptors (CBs) and their involvement in the development of metabolic and hormonal changes in rats fed a fructose-rich diet (F). Methods: In normal rat islets, we determined CBs (immunofluorescence and RT-PCR) and glucose-stimulated insulin secretion (GSIS) of isolated islets incubated with the CB1 antagonist rimonabant (R) and/or different CBs agonists. In 3-week F-fed rats, we determined the in vivo effect of R upon serum glucose, triglyceride and insulin levels, HOMA-IR, GSIS and CBs and IRS gene expression levels (real-time PCR). Results: CBs appeared exclusively in islet α-cells. Whereas different CBs agonists enhanced GSIS in normal rat islets, R did not affect it. F rats had higher serum triglyceride and insulin levels and HOMA-IR than control rats; these alterations were prevented by R coadministration. Although R did not correct the increased GSIS observed in F islets, it modulated CBs and IRS gene expression. Conclusion: Islet CBs would exert an important modulatory role in metabolic homeostasis. Administration of R and F affected islet CBs expression and prevented the development of Finduced metabolic impairment. Selective islet CB1 blockers could be useful to prevent/treat the alterations induced by the intake of unbalanced/unhealthy diets.
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Flores, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina
Fil: Alzugaray, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico la Plata; Argentina
Fil: Cubilla, Marisa Angelica. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina
Fil: Raschia, Maria A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Román, Carolina Lisi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata. Centro de Endocrinologia Experimental y Aplicada (i); Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
description Objectives: To determine the cellular distribution of islet cannabinoid receptors (CBs) and their involvement in the development of metabolic and hormonal changes in rats fed a fructose-rich diet (F). Methods: In normal rat islets, we determined CBs (immunofluorescence and RT-PCR) and glucose-stimulated insulin secretion (GSIS) of isolated islets incubated with the CB1 antagonist rimonabant (R) and/or different CBs agonists. In 3-week F-fed rats, we determined the in vivo effect of R upon serum glucose, triglyceride and insulin levels, HOMA-IR, GSIS and CBs and IRS gene expression levels (real-time PCR). Results: CBs appeared exclusively in islet α-cells. Whereas different CBs agonists enhanced GSIS in normal rat islets, R did not affect it. F rats had higher serum triglyceride and insulin levels and HOMA-IR than control rats; these alterations were prevented by R coadministration. Although R did not correct the increased GSIS observed in F islets, it modulated CBs and IRS gene expression. Conclusion: Islet CBs would exert an important modulatory role in metabolic homeostasis. Administration of R and F affected islet CBs expression and prevented the development of Finduced metabolic impairment. Selective islet CB1 blockers could be useful to prevent/treat the alterations induced by the intake of unbalanced/unhealthy diets.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3405
Gagliardino, Juan Jose; Flores, Luis Emilio; Alzugaray, Maria Eugenia; Cubilla, Marisa Angelica; Raschia, Maria A.; et al.; Islet Cannabinoid Receptors Cellular Distribution and Biological Function; Lippincott, Williams; Pancreas; 42; 10-2013; 1085-1092
0885-3177
url http://hdl.handle.net/11336/3405
identifier_str_mv Gagliardino, Juan Jose; Flores, Luis Emilio; Alzugaray, Maria Eugenia; Cubilla, Marisa Angelica; Raschia, Maria A.; et al.; Islet Cannabinoid Receptors Cellular Distribution and Biological Function; Lippincott, Williams; Pancreas; 42; 10-2013; 1085-1092
0885-3177
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/24005231
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/msword
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott, Williams
publisher.none.fl_str_mv Lippincott, Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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