Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro

Autores
Jimenez, Luis Emanuel; Roldan Olarte, Eugenia Mariela; Alvarez, Rosa Maria Susana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The process of in vitro oocyte maturation, both at nuclear and cytoplasmic level, is a crucial stage to the success of subsequent events, such as the in vitro fertilization and the early embryonic development. During this process an increase and redistribution of organelles occurs which leads to higher lipid and glutation contents. In addition, the biochemical profile of the cytoplasm experiences significant changes, mainly due to the increase in transcription and the consequent protein synthesis. The necessary energy for these events is provided by an increased glycolysis (1).The aim of this work is to characterize the chemical composition of the cytoplasm of in vitro matured bovine oocytes (IVM) by using Raman microscopy. We focus on the spectral behavior of characteristic bands of DNA, RNA, proteins, carbohydrates and lipids.The Raman spectra were acquired from five different points of the cytoplasm of each whole oocyte (n=16). In order to minimize the contribution from the molecules of the Zona Pellucida (ZP), the ZP spectrum was subtracted from the cytoplasm spectra. The vibrational characterization of the ZP glycoproteins has been previously performed by our group (2). We used the band corresponding to the vibration of sialic acid, component of the ZP glycoproteins, as a reference for the spectral subtraction. The resulting spectra show high phospholipid content and a significant absence ofsugar bands in the IVM cytoplasm. These results are in accordance with the vesicle formation and the expenditure of energy reserves that led to the matured oocyte status.
Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de Biofísica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biofísica
Materia
RAMAN MICRO-SPECTROSCOPY
OOCYTES
CYTOPLASM
BOVINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238715
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/238715
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitroJimenez, Luis EmanuelRoldan Olarte, Eugenia MarielaAlvarez, Rosa Maria SusanaRAMAN MICRO-SPECTROSCOPYOOCYTESCYTOPLASMBOVINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The process of in vitro oocyte maturation, both at nuclear and cytoplasmic level, is a crucial stage to the success of subsequent events, such as the in vitro fertilization and the early embryonic development. During this process an increase and redistribution of organelles occurs which leads to higher lipid and glutation contents. In addition, the biochemical profile of the cytoplasm experiences significant changes, mainly due to the increase in transcription and the consequent protein synthesis. The necessary energy for these events is provided by an increased glycolysis (1).The aim of this work is to characterize the chemical composition of the cytoplasm of in vitro matured bovine oocytes (IVM) by using Raman microscopy. We focus on the spectral behavior of characteristic bands of DNA, RNA, proteins, carbohydrates and lipids.The Raman spectra were acquired from five different points of the cytoplasm of each whole oocyte (n=16). In order to minimize the contribution from the molecules of the Zona Pellucida (ZP), the ZP spectrum was subtracted from the cytoplasm spectra. The vibrational characterization of the ZP glycoproteins has been previously performed by our group (2). We used the band corresponding to the vibration of sialic acid, component of the ZP glycoproteins, as a reference for the spectral subtraction. The resulting spectra show high phospholipid content and a significant absence ofsugar bands in the IVM cytoplasm. These results are in accordance with the vesicle formation and the expenditure of energy reserves that led to the matured oocyte status.Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaIII Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de BiofísicaSan Miguel de TucumánArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/238715Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 348-349CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:48Zoai:ri.conicet.gov.ar:11336/238715instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:48.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
title Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
spellingShingle Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
Jimenez, Luis Emanuel
RAMAN MICRO-SPECTROSCOPY
OOCYTES
CYTOPLASM
BOVINE
title_short Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
title_full Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
title_fullStr Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
title_full_unstemmed Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
title_sort Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro
dc.creator.none.fl_str_mv Jimenez, Luis Emanuel
Roldan Olarte, Eugenia Mariela
Alvarez, Rosa Maria Susana
author Jimenez, Luis Emanuel
author_facet Jimenez, Luis Emanuel
Roldan Olarte, Eugenia Mariela
Alvarez, Rosa Maria Susana
author_role author
author2 Roldan Olarte, Eugenia Mariela
Alvarez, Rosa Maria Susana
author2_role author
author
dc.subject.none.fl_str_mv RAMAN MICRO-SPECTROSCOPY
OOCYTES
CYTOPLASM
BOVINE
topic RAMAN MICRO-SPECTROSCOPY
OOCYTES
CYTOPLASM
BOVINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The process of in vitro oocyte maturation, both at nuclear and cytoplasmic level, is a crucial stage to the success of subsequent events, such as the in vitro fertilization and the early embryonic development. During this process an increase and redistribution of organelles occurs which leads to higher lipid and glutation contents. In addition, the biochemical profile of the cytoplasm experiences significant changes, mainly due to the increase in transcription and the consequent protein synthesis. The necessary energy for these events is provided by an increased glycolysis (1).The aim of this work is to characterize the chemical composition of the cytoplasm of in vitro matured bovine oocytes (IVM) by using Raman microscopy. We focus on the spectral behavior of characteristic bands of DNA, RNA, proteins, carbohydrates and lipids.The Raman spectra were acquired from five different points of the cytoplasm of each whole oocyte (n=16). In order to minimize the contribution from the molecules of the Zona Pellucida (ZP), the ZP spectrum was subtracted from the cytoplasm spectra. The vibrational characterization of the ZP glycoproteins has been previously performed by our group (2). We used the band corresponding to the vibration of sialic acid, component of the ZP glycoproteins, as a reference for the spectral subtraction. The resulting spectra show high phospholipid content and a significant absence ofsugar bands in the IVM cytoplasm. These results are in accordance with the vesicle formation and the expenditure of energy reserves that led to the matured oocyte status.
Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de Biofísica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biofísica
description The process of in vitro oocyte maturation, both at nuclear and cytoplasmic level, is a crucial stage to the success of subsequent events, such as the in vitro fertilization and the early embryonic development. During this process an increase and redistribution of organelles occurs which leads to higher lipid and glutation contents. In addition, the biochemical profile of the cytoplasm experiences significant changes, mainly due to the increase in transcription and the consequent protein synthesis. The necessary energy for these events is provided by an increased glycolysis (1).The aim of this work is to characterize the chemical composition of the cytoplasm of in vitro matured bovine oocytes (IVM) by using Raman microscopy. We focus on the spectral behavior of characteristic bands of DNA, RNA, proteins, carbohydrates and lipids.The Raman spectra were acquired from five different points of the cytoplasm of each whole oocyte (n=16). In order to minimize the contribution from the molecules of the Zona Pellucida (ZP), the ZP spectrum was subtracted from the cytoplasm spectra. The vibrational characterization of the ZP glycoproteins has been previously performed by our group (2). We used the band corresponding to the vibration of sialic acid, component of the ZP glycoproteins, as a reference for the spectral subtraction. The resulting spectra show high phospholipid content and a significant absence ofsugar bands in the IVM cytoplasm. These results are in accordance with the vesicle formation and the expenditure of energy reserves that led to the matured oocyte status.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238715
Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 348-349
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238715
identifier_str_mv Raman micro-Spectroscopy application for studying of the components of Cytoplasm bovine oocytes matured in vitro; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual de la Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 348-349
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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