In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis

Autores
Jimenez, Luis Emanuel; Roldan Olarte, Eugenia Mariela; Dominguez, Alfredo Nicolás; Alvarez, Rosa Maria Susana
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Raman microscopy is a powerful tool for examining mammalian female gametes and identifying molecular markers to evaluate their competence after in vitro maturation (IVM). However, its application to comprehensively analyze the biochemical changes induced in oocytes by vascular endothelial growth factor D (VEGF–D) enrichment during IVM remains largely unexplored, although various media supplements are commonly used to optimize IVM protocols. This study presents the first application of Raman spectroscopy to investigate the effect of VEGF–D supplementation on the biochemical composition of in vitro matured bovine oocytes. Spectral profiles of zona pellucida-free oocytes matured for 18 and 22 hours in standard and VEGF–Denriched media were compared. The differences in specific marker bands were correlated with the attained maturational status. Multivariate statistical analysis supported the spectral interpretations, revealing an increased overall content of proteins, lipids, and carbohydrates in the cytoplasm of oocytes matured in the presence of VEGF–D. This sensitive methodology shows promise for improving culture conditions and enhancing in vitro maturation rates in future research.
Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
Fil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina
Fil: Dominguez, Alfredo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Orgánica; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
Materia
CYTOPLASMATIC MATURATION
ZP–FREE OOCYTES
SUPPLEMENTED MEDIUM
MULTIVARIATE ANALYSIS
RAMAN MICROSCOPY
GROWTH FACTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263738
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263738
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysisJimenez, Luis EmanuelRoldan Olarte, Eugenia MarielaDominguez, Alfredo NicolásAlvarez, Rosa Maria SusanaCYTOPLASMATIC MATURATIONZP–FREE OOCYTESSUPPLEMENTED MEDIUMMULTIVARIATE ANALYSISRAMAN MICROSCOPYGROWTH FACTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Raman microscopy is a powerful tool for examining mammalian female gametes and identifying molecular markers to evaluate their competence after in vitro maturation (IVM). However, its application to comprehensively analyze the biochemical changes induced in oocytes by vascular endothelial growth factor D (VEGF–D) enrichment during IVM remains largely unexplored, although various media supplements are commonly used to optimize IVM protocols. This study presents the first application of Raman spectroscopy to investigate the effect of VEGF–D supplementation on the biochemical composition of in vitro matured bovine oocytes. Spectral profiles of zona pellucida-free oocytes matured for 18 and 22 hours in standard and VEGF–Denriched media were compared. The differences in specific marker bands were correlated with the attained maturational status. Multivariate statistical analysis supported the spectral interpretations, revealing an increased overall content of proteins, lipids, and carbohydrates in the cytoplasm of oocytes matured in the presence of VEGF–D. This sensitive methodology shows promise for improving culture conditions and enhancing in vitro maturation rates in future research.Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; ArgentinaFil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; ArgentinaFil: Dominguez, Alfredo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Orgánica; ArgentinaFil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; ArgentinaGerman Multidisciplinary Publishing Center2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263738Jimenez, Luis Emanuel; Roldan Olarte, Eugenia Mariela; Dominguez, Alfredo Nicolás; Alvarez, Rosa Maria Susana; In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 1; 1-2025; 62-752703-1322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://gmpc-akademie.de/articles/gjvr/single/239info:eu-repo/semantics/altIdentifier/doi/10.51585/gjvr.2025.1.0120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:41Zoai:ri.conicet.gov.ar:11336/263738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:42.109CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
title In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
spellingShingle In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
Jimenez, Luis Emanuel
CYTOPLASMATIC MATURATION
ZP–FREE OOCYTES
SUPPLEMENTED MEDIUM
MULTIVARIATE ANALYSIS
RAMAN MICROSCOPY
GROWTH FACTORS
title_short In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
title_full In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
title_fullStr In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
title_full_unstemmed In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
title_sort In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis
dc.creator.none.fl_str_mv Jimenez, Luis Emanuel
Roldan Olarte, Eugenia Mariela
Dominguez, Alfredo Nicolás
Alvarez, Rosa Maria Susana
author Jimenez, Luis Emanuel
author_facet Jimenez, Luis Emanuel
Roldan Olarte, Eugenia Mariela
Dominguez, Alfredo Nicolás
Alvarez, Rosa Maria Susana
author_role author
author2 Roldan Olarte, Eugenia Mariela
Dominguez, Alfredo Nicolás
Alvarez, Rosa Maria Susana
author2_role author
author
author
dc.subject.none.fl_str_mv CYTOPLASMATIC MATURATION
ZP–FREE OOCYTES
SUPPLEMENTED MEDIUM
MULTIVARIATE ANALYSIS
RAMAN MICROSCOPY
GROWTH FACTORS
topic CYTOPLASMATIC MATURATION
ZP–FREE OOCYTES
SUPPLEMENTED MEDIUM
MULTIVARIATE ANALYSIS
RAMAN MICROSCOPY
GROWTH FACTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Raman microscopy is a powerful tool for examining mammalian female gametes and identifying molecular markers to evaluate their competence after in vitro maturation (IVM). However, its application to comprehensively analyze the biochemical changes induced in oocytes by vascular endothelial growth factor D (VEGF–D) enrichment during IVM remains largely unexplored, although various media supplements are commonly used to optimize IVM protocols. This study presents the first application of Raman spectroscopy to investigate the effect of VEGF–D supplementation on the biochemical composition of in vitro matured bovine oocytes. Spectral profiles of zona pellucida-free oocytes matured for 18 and 22 hours in standard and VEGF–Denriched media were compared. The differences in specific marker bands were correlated with the attained maturational status. Multivariate statistical analysis supported the spectral interpretations, revealing an increased overall content of proteins, lipids, and carbohydrates in the cytoplasm of oocytes matured in the presence of VEGF–D. This sensitive methodology shows promise for improving culture conditions and enhancing in vitro maturation rates in future research.
Fil: Jimenez, Luis Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
Fil: Roldan Olarte, Eugenia Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina
Fil: Dominguez, Alfredo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Orgánica; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
description Raman microscopy is a powerful tool for examining mammalian female gametes and identifying molecular markers to evaluate their competence after in vitro maturation (IVM). However, its application to comprehensively analyze the biochemical changes induced in oocytes by vascular endothelial growth factor D (VEGF–D) enrichment during IVM remains largely unexplored, although various media supplements are commonly used to optimize IVM protocols. This study presents the first application of Raman spectroscopy to investigate the effect of VEGF–D supplementation on the biochemical composition of in vitro matured bovine oocytes. Spectral profiles of zona pellucida-free oocytes matured for 18 and 22 hours in standard and VEGF–Denriched media were compared. The differences in specific marker bands were correlated with the attained maturational status. Multivariate statistical analysis supported the spectral interpretations, revealing an increased overall content of proteins, lipids, and carbohydrates in the cytoplasm of oocytes matured in the presence of VEGF–D. This sensitive methodology shows promise for improving culture conditions and enhancing in vitro maturation rates in future research.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263738
Jimenez, Luis Emanuel; Roldan Olarte, Eugenia Mariela; Dominguez, Alfredo Nicolás; Alvarez, Rosa Maria Susana; In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 1; 1-2025; 62-75
2703-1322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263738
identifier_str_mv Jimenez, Luis Emanuel; Roldan Olarte, Eugenia Mariela; Dominguez, Alfredo Nicolás; Alvarez, Rosa Maria Susana; In vitro matured bovine oocytes in the presence of vascular endothelial growth factor–D: A Raman spectroscopy analysis; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 1; 1-2025; 62-75
2703-1322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://gmpc-akademie.de/articles/gjvr/single/239
info:eu-repo/semantics/altIdentifier/doi/10.51585/gjvr.2025.1.0120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv German Multidisciplinary Publishing Center
publisher.none.fl_str_mv German Multidisciplinary Publishing Center
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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