Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis

Autores
Rivoira, Maria Angelica; Marchionatti, Ana María; Centeno, Viviana Andrea; Díaz de Barboza, Gabriela Edith; Peralta López, María Elena; Tolosa, Nori Graciela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca2+ absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca2+ absorption was measured as well as the gene and protein expressions of molecules involved in the Ca2+ transcellular pathway. NaDOC inhibited the intestinal Ca2+ absorption, which was concentration dependent. Ca2+-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca2+-ATPase, calbindin D28k and Na+/Ca2+ exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca2+ absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca2+ absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis. © 2012 Elsevier Inc.
Fil: Rivoira, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Marchionatti, Ana María. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Díaz de Barboza, Gabriela Edith. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Peralta López, María Elena. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
APOPTOSIS
INTESTINAL CALCIUM ABSORPTION
OXIDATIVE STRESS
SODIUM DEOXYCHOLATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133934
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/133934
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosisRivoira, Maria AngelicaMarchionatti, Ana MaríaCenteno, Viviana AndreaDíaz de Barboza, Gabriela EdithPeralta López, María ElenaTolosa, Nori GracielaAPOPTOSISINTESTINAL CALCIUM ABSORPTIONOXIDATIVE STRESSSODIUM DEOXYCHOLATEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca2+ absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca2+ absorption was measured as well as the gene and protein expressions of molecules involved in the Ca2+ transcellular pathway. NaDOC inhibited the intestinal Ca2+ absorption, which was concentration dependent. Ca2+-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca2+-ATPase, calbindin D28k and Na+/Ca2+ exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca2+ absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca2+ absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis. © 2012 Elsevier Inc.Fil: Rivoira, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaFil: Marchionatti, Ana María. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaFil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaFil: Díaz de Barboza, Gabriela Edith. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaFil: Peralta López, María Elena. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaElsevier Science Inc2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133934Rivoira, Maria Angelica; Marchionatti, Ana María; Centeno, Viviana Andrea; Díaz de Barboza, Gabriela Edith; Peralta López, María Elena; et al.; Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 162; 4; 5-2012; 397-4051095-6433CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2012.04.016info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1095643312001158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:34Zoai:ri.conicet.gov.ar:11336/133934instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:34.98CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
title Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
spellingShingle Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
Rivoira, Maria Angelica
APOPTOSIS
INTESTINAL CALCIUM ABSORPTION
OXIDATIVE STRESS
SODIUM DEOXYCHOLATE
title_short Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
title_full Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
title_fullStr Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
title_full_unstemmed Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
title_sort Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
dc.creator.none.fl_str_mv Rivoira, Maria Angelica
Marchionatti, Ana María
Centeno, Viviana Andrea
Díaz de Barboza, Gabriela Edith
Peralta López, María Elena
Tolosa, Nori Graciela
author Rivoira, Maria Angelica
author_facet Rivoira, Maria Angelica
Marchionatti, Ana María
Centeno, Viviana Andrea
Díaz de Barboza, Gabriela Edith
Peralta López, María Elena
Tolosa, Nori Graciela
author_role author
author2 Marchionatti, Ana María
Centeno, Viviana Andrea
Díaz de Barboza, Gabriela Edith
Peralta López, María Elena
Tolosa, Nori Graciela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
INTESTINAL CALCIUM ABSORPTION
OXIDATIVE STRESS
SODIUM DEOXYCHOLATE
topic APOPTOSIS
INTESTINAL CALCIUM ABSORPTION
OXIDATIVE STRESS
SODIUM DEOXYCHOLATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca2+ absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca2+ absorption was measured as well as the gene and protein expressions of molecules involved in the Ca2+ transcellular pathway. NaDOC inhibited the intestinal Ca2+ absorption, which was concentration dependent. Ca2+-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca2+-ATPase, calbindin D28k and Na+/Ca2+ exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca2+ absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca2+ absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis. © 2012 Elsevier Inc.
Fil: Rivoira, Maria Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Marchionatti, Ana María. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Díaz de Barboza, Gabriela Edith. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Peralta López, María Elena. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca2+ absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca2+ absorption was measured as well as the gene and protein expressions of molecules involved in the Ca2+ transcellular pathway. NaDOC inhibited the intestinal Ca2+ absorption, which was concentration dependent. Ca2+-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca2+-ATPase, calbindin D28k and Na+/Ca2+ exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca2+ absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca2+ absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis. © 2012 Elsevier Inc.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133934
Rivoira, Maria Angelica; Marchionatti, Ana María; Centeno, Viviana Andrea; Díaz de Barboza, Gabriela Edith; Peralta López, María Elena; et al.; Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 162; 4; 5-2012; 397-405
1095-6433
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133934
identifier_str_mv Rivoira, Maria Angelica; Marchionatti, Ana María; Centeno, Viviana Andrea; Díaz de Barboza, Gabriela Edith; Peralta López, María Elena; et al.; Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 162; 4; 5-2012; 397-405
1095-6433
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2012.04.016
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1095643312001158
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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