Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis

Autores
Rivas, Maria Gabriela; Carepo, Marta S.P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; Lopes, Ana T.; Brondino, Carlos Dante; Pereira, Alice S.; George, Graham N.; Dolla, Alain; Moura, José J.G.; Moura, Isabel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 ± 13 kDa) consisting of 16-18 monomers of 15321.1 ± 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M-1 cm-1, respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmental conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 μM molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed.
Fil: Rivas, Maria Gabriela. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Carepo, Marta S.P.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Mota, Cristiano S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Korbas, Malgorzata. University of Saskatchewan; Canadá
Fil: Durand, Marie-Claire. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; Francia
Fil: Lopes, Ana T.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina
Fil: Pereira, Alice S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: George, Graham N.. University of Saskatchewan; Canadá
Fil: Dolla, Alain. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; Francia
Fil: Moura, José J.G.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Moura, Isabel. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Materia
MOLYBDENUM-CONTAINING ENZYMES
METAL TOLERANCE
DESULFOVIBRIO
HETEROMETALLIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97872
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensisRivas, Maria GabrielaCarepo, Marta S.P.Mota, Cristiano S.Korbas, MalgorzataDurand, Marie-ClaireLopes, Ana T.Brondino, Carlos DantePereira, Alice S.George, Graham N.Dolla, AlainMoura, José J.G.Moura, IsabelMOLYBDENUM-CONTAINING ENZYMESMETAL TOLERANCEDESULFOVIBRIOHETEROMETALLIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 ± 13 kDa) consisting of 16-18 monomers of 15321.1 ± 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M-1 cm-1, respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmental conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 μM molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed.Fil: Rivas, Maria Gabriela. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: Carepo, Marta S.P.. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: Mota, Cristiano S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: Korbas, Malgorzata. University of Saskatchewan; CanadáFil: Durand, Marie-Claire. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; FranciaFil: Lopes, Ana T.. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: Brondino, Carlos Dante. Universidad Nacional del Litoral; ArgentinaFil: Pereira, Alice S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: George, Graham N.. University of Saskatchewan; CanadáFil: Dolla, Alain. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; FranciaFil: Moura, José J.G.. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalFil: Moura, Isabel. Requimte/cqfb, Departamento de Quimica, Fct-unl; PortugalAmerican Chemical Society2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97872Rivas, Maria Gabriela; Carepo, Marta S.P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; et al.; Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis; American Chemical Society; Biochemistry; 48; 5; 2-2009; 873-8820006-2960CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bi801773tinfo:eu-repo/semantics/altIdentifier/doi/10.1021/bi801773tinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:43Zoai:ri.conicet.gov.ar:11336/97872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:43.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
title Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
spellingShingle Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
Rivas, Maria Gabriela
MOLYBDENUM-CONTAINING ENZYMES
METAL TOLERANCE
DESULFOVIBRIO
HETEROMETALLIC
title_short Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
title_full Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
title_fullStr Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
title_full_unstemmed Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
title_sort Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis
dc.creator.none.fl_str_mv Rivas, Maria Gabriela
Carepo, Marta S.P.
Mota, Cristiano S.
Korbas, Malgorzata
Durand, Marie-Claire
Lopes, Ana T.
Brondino, Carlos Dante
Pereira, Alice S.
George, Graham N.
Dolla, Alain
Moura, José J.G.
Moura, Isabel
author Rivas, Maria Gabriela
author_facet Rivas, Maria Gabriela
Carepo, Marta S.P.
Mota, Cristiano S.
Korbas, Malgorzata
Durand, Marie-Claire
Lopes, Ana T.
Brondino, Carlos Dante
Pereira, Alice S.
George, Graham N.
Dolla, Alain
Moura, José J.G.
Moura, Isabel
author_role author
author2 Carepo, Marta S.P.
Mota, Cristiano S.
Korbas, Malgorzata
Durand, Marie-Claire
Lopes, Ana T.
Brondino, Carlos Dante
Pereira, Alice S.
George, Graham N.
Dolla, Alain
Moura, José J.G.
Moura, Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MOLYBDENUM-CONTAINING ENZYMES
METAL TOLERANCE
DESULFOVIBRIO
HETEROMETALLIC
topic MOLYBDENUM-CONTAINING ENZYMES
METAL TOLERANCE
DESULFOVIBRIO
HETEROMETALLIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 ± 13 kDa) consisting of 16-18 monomers of 15321.1 ± 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M-1 cm-1, respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmental conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 μM molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed.
Fil: Rivas, Maria Gabriela. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Carepo, Marta S.P.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Mota, Cristiano S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Korbas, Malgorzata. University of Saskatchewan; Canadá
Fil: Durand, Marie-Claire. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; Francia
Fil: Lopes, Ana T.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina
Fil: Pereira, Alice S.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: George, Graham N.. University of Saskatchewan; Canadá
Fil: Dolla, Alain. Cnrs-imm, Unité Interactions Et Modulateurs de Réponses; Francia
Fil: Moura, José J.G.. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
Fil: Moura, Isabel. Requimte/cqfb, Departamento de Quimica, Fct-unl; Portugal
description The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 ± 13 kDa) consisting of 16-18 monomers of 15321.1 ± 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M-1 cm-1, respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmental conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 μM molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97872
Rivas, Maria Gabriela; Carepo, Marta S.P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; et al.; Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis; American Chemical Society; Biochemistry; 48; 5; 2-2009; 873-882
0006-2960
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97872
identifier_str_mv Rivas, Maria Gabriela; Carepo, Marta S.P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; et al.; Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis; American Chemical Society; Biochemistry; 48; 5; 2-2009; 873-882
0006-2960
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bi801773t
info:eu-repo/semantics/altIdentifier/doi/10.1021/bi801773t
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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