Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release

Autores
Theas, Maria Susana; de Laurentiis, Andrea; Candolfi, Marianela; López, Silvia Liliana; Carrasco, Andrés E.; Zaldivar, Verónica; Seilicovich, Adriana
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that markedly affects neuroendocrine functions. This cytokine is expressed in the anterior pituitary where its receptors are also present. Nitric oxide (NO) is synthesized in gonadotropes and folliculo-stellate cells of the anterior pituitary. Since NO directly inhibits prolactin secretion, we investigated the involvement of NO in the inhibitory effect of TNF-α on prolactin release from anterior pituitary cells of female rats. The presence of L-NAME (1 mM), an inhibitor of NO synthase (NOS), in the incubation medium significantly blunted the inhibition of prolactin release produced by TNF-α (50 ng/ml). TNF-α increased nitrite release to the incubation medium. The activity of NOS as measured by [14C]citrulline production was significantly enhanced when anterior pituitary cells were incubated with TNF-α for 8 h or more. Also, TNF-α induced iNOS gene expression in anterior pituitary cells as assessed by reverse transcriptase-polymerase chain reaction. The current results indicate that NO is involved in the inhibitory effect of TNF-α on prolactin secretion and that TNF-α induces iNOS transcription and stimulates NO synthesis in anterior pituitary cells.
Fil: Theas, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Candolfi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: López, Silvia Liliana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Carrasco, Andrés E.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zaldivar, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
CYTOKINES
MOLECULAR NEUROENDOCRINOLOGY
NITRIC OXIDE
NITRIC OXIDE SYNTHASE
PROLACTIN
TUMOR NECROSIS FACTORS-Α
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125319
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/125319
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin releaseTheas, Maria Susanade Laurentiis, AndreaCandolfi, MarianelaLópez, Silvia LilianaCarrasco, Andrés E.Zaldivar, VerónicaSeilicovich, AdrianaCYTOKINESMOLECULAR NEUROENDOCRINOLOGYNITRIC OXIDENITRIC OXIDE SYNTHASEPROLACTINTUMOR NECROSIS FACTORS-Αhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that markedly affects neuroendocrine functions. This cytokine is expressed in the anterior pituitary where its receptors are also present. Nitric oxide (NO) is synthesized in gonadotropes and folliculo-stellate cells of the anterior pituitary. Since NO directly inhibits prolactin secretion, we investigated the involvement of NO in the inhibitory effect of TNF-α on prolactin release from anterior pituitary cells of female rats. The presence of L-NAME (1 mM), an inhibitor of NO synthase (NOS), in the incubation medium significantly blunted the inhibition of prolactin release produced by TNF-α (50 ng/ml). TNF-α increased nitrite release to the incubation medium. The activity of NOS as measured by [14C]citrulline production was significantly enhanced when anterior pituitary cells were incubated with TNF-α for 8 h or more. Also, TNF-α induced iNOS gene expression in anterior pituitary cells as assessed by reverse transcriptase-polymerase chain reaction. The current results indicate that NO is involved in the inhibitory effect of TNF-α on prolactin secretion and that TNF-α induces iNOS transcription and stimulates NO synthesis in anterior pituitary cells.Fil: Theas, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Candolfi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: López, Silvia Liliana. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Carrasco, Andrés E.. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Zaldivar, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaKarger2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125319Theas, Maria Susana; de Laurentiis, Andrea; Candolfi, Marianela; López, Silvia Liliana; Carrasco, Andrés E.; et al.; Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release; Karger; Neuroendocrinology; 74; 2; 12-2001; 82-860028-3835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000054673info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/11474215/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:49:31Zoai:ri.conicet.gov.ar:11336/125319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:49:31.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
title Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
spellingShingle Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
Theas, Maria Susana
CYTOKINES
MOLECULAR NEUROENDOCRINOLOGY
NITRIC OXIDE
NITRIC OXIDE SYNTHASE
PROLACTIN
TUMOR NECROSIS FACTORS-Α
title_short Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
title_full Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
title_fullStr Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
title_full_unstemmed Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
title_sort Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release
dc.creator.none.fl_str_mv Theas, Maria Susana
de Laurentiis, Andrea
Candolfi, Marianela
López, Silvia Liliana
Carrasco, Andrés E.
Zaldivar, Verónica
Seilicovich, Adriana
author Theas, Maria Susana
author_facet Theas, Maria Susana
de Laurentiis, Andrea
Candolfi, Marianela
López, Silvia Liliana
Carrasco, Andrés E.
Zaldivar, Verónica
Seilicovich, Adriana
author_role author
author2 de Laurentiis, Andrea
Candolfi, Marianela
López, Silvia Liliana
Carrasco, Andrés E.
Zaldivar, Verónica
Seilicovich, Adriana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CYTOKINES
MOLECULAR NEUROENDOCRINOLOGY
NITRIC OXIDE
NITRIC OXIDE SYNTHASE
PROLACTIN
TUMOR NECROSIS FACTORS-Α
topic CYTOKINES
MOLECULAR NEUROENDOCRINOLOGY
NITRIC OXIDE
NITRIC OXIDE SYNTHASE
PROLACTIN
TUMOR NECROSIS FACTORS-Α
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that markedly affects neuroendocrine functions. This cytokine is expressed in the anterior pituitary where its receptors are also present. Nitric oxide (NO) is synthesized in gonadotropes and folliculo-stellate cells of the anterior pituitary. Since NO directly inhibits prolactin secretion, we investigated the involvement of NO in the inhibitory effect of TNF-α on prolactin release from anterior pituitary cells of female rats. The presence of L-NAME (1 mM), an inhibitor of NO synthase (NOS), in the incubation medium significantly blunted the inhibition of prolactin release produced by TNF-α (50 ng/ml). TNF-α increased nitrite release to the incubation medium. The activity of NOS as measured by [14C]citrulline production was significantly enhanced when anterior pituitary cells were incubated with TNF-α for 8 h or more. Also, TNF-α induced iNOS gene expression in anterior pituitary cells as assessed by reverse transcriptase-polymerase chain reaction. The current results indicate that NO is involved in the inhibitory effect of TNF-α on prolactin secretion and that TNF-α induces iNOS transcription and stimulates NO synthesis in anterior pituitary cells.
Fil: Theas, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Candolfi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: López, Silvia Liliana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Carrasco, Andrés E.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zaldivar, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that markedly affects neuroendocrine functions. This cytokine is expressed in the anterior pituitary where its receptors are also present. Nitric oxide (NO) is synthesized in gonadotropes and folliculo-stellate cells of the anterior pituitary. Since NO directly inhibits prolactin secretion, we investigated the involvement of NO in the inhibitory effect of TNF-α on prolactin release from anterior pituitary cells of female rats. The presence of L-NAME (1 mM), an inhibitor of NO synthase (NOS), in the incubation medium significantly blunted the inhibition of prolactin release produced by TNF-α (50 ng/ml). TNF-α increased nitrite release to the incubation medium. The activity of NOS as measured by [14C]citrulline production was significantly enhanced when anterior pituitary cells were incubated with TNF-α for 8 h or more. Also, TNF-α induced iNOS gene expression in anterior pituitary cells as assessed by reverse transcriptase-polymerase chain reaction. The current results indicate that NO is involved in the inhibitory effect of TNF-α on prolactin secretion and that TNF-α induces iNOS transcription and stimulates NO synthesis in anterior pituitary cells.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125319
Theas, Maria Susana; de Laurentiis, Andrea; Candolfi, Marianela; López, Silvia Liliana; Carrasco, Andrés E.; et al.; Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release; Karger; Neuroendocrinology; 74; 2; 12-2001; 82-86
0028-3835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125319
identifier_str_mv Theas, Maria Susana; de Laurentiis, Andrea; Candolfi, Marianela; López, Silvia Liliana; Carrasco, Andrés E.; et al.; Nitric oxide mediates the inhibitory effect of tumor necrosis factor-α on prolactin release; Karger; Neuroendocrinology; 74; 2; 12-2001; 82-86
0028-3835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000054673
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/11474215/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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