Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development

Autores
Flores, Teresita; Todd, Christopher; Tovar Mendez, Alejandro; Dhanoa, Preetinder; Correa Aragunde, Maria Natalia; Hoyos, Mary Elizabeth; Brownfield, Disa M.; Mullen, Robert T.; Lamattina, Lorenzo; Polacco, Joe
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mutation of either arginase structural gene (ARGAH1 or ARGAH2 encoding arginine [Arg] amidohydrolase-1 and -2, respectively) resulted in increased formation of lateral and adventitious roots in Arabidopsis (Arabidopsis thaliana) seedlings and increased nitric oxide (NO) accumulation and efflux, detected by the fluorogenic traps 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate and diamino-rhodamine-4M, respectively. Upon seedling exposure to the synthetic auxin naphthaleneacetic acid, NO accumulation was differentially enhanced in argah1-1 and argah2-1 compared with the wild type. In all genotypes, much 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate fluorescence originated from mitochondria. The arginases are both localized to the mitochondrial matrix and closely related. However, their expression levels and patterns differ: ARGAH1 encoded the minor activity, and ARGAH1-driven β-glucuronidase (GUS) was expressed throughout the seedling; the ARGAH2::GUS expression pattern was more localized. Naphthaleneacetic acid increased seedling lateral root numbers (total lateral roots per primary root) in the mutants to twice the number in the wild type, consistent with increased internal NO leading to enhanced auxin signaling in roots. In agreement, argah1-1 and argah2-1 showed increased expression of the auxin-responsive reporter DR5::GUS in root tips, emerging lateral roots, and hypocotyls. We propose that Arg, or an Arg derivative, is a potential NO source and that reduced arginase activity in the mutants results in greater conversion of Arg to NO, thereby potentiating auxin action in roots. This model is supported by supplemental Arg induction of adventitious roots and increased NO accumulation in argah1-1 and argah2-1 versus the wild type.
Fil: Flores, Teresita. University of Missouri; Estados Unidos
Fil: Todd, Christopher. University of Saskatchewan; Canadá. University of Missouri; Estados Unidos
Fil: Tovar Mendez, Alejandro. University of Missouri; Estados Unidos
Fil: Dhanoa, Preetinder. University of Guelph; Canadá
Fil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hoyos, Mary Elizabeth. University of Missouri; Estados Unidos
Fil: Brownfield, Disa M.. University of Alberta; Canadá
Fil: Mullen, Robert T.. University of Guelph; Canadá
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Polacco, Joe. Universidade Federal do Rio Grande do Sul; Brasil
Materia
arginase
nitric oxide
roots
nitric oxide synthase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133922
Acceder
id CONICETDig_bfb42b8efe4d750373366bd1429f12f4
oai_identifier_str oai:ri.conicet.gov.ar:11336/133922
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root developmentFlores, TeresitaTodd, ChristopherTovar Mendez, AlejandroDhanoa, PreetinderCorrea Aragunde, Maria NataliaHoyos, Mary ElizabethBrownfield, Disa M.Mullen, Robert T.Lamattina, LorenzoPolacco, Joearginasenitric oxiderootsnitric oxide synthasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mutation of either arginase structural gene (ARGAH1 or ARGAH2 encoding arginine [Arg] amidohydrolase-1 and -2, respectively) resulted in increased formation of lateral and adventitious roots in Arabidopsis (Arabidopsis thaliana) seedlings and increased nitric oxide (NO) accumulation and efflux, detected by the fluorogenic traps 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate and diamino-rhodamine-4M, respectively. Upon seedling exposure to the synthetic auxin naphthaleneacetic acid, NO accumulation was differentially enhanced in argah1-1 and argah2-1 compared with the wild type. In all genotypes, much 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate fluorescence originated from mitochondria. The arginases are both localized to the mitochondrial matrix and closely related. However, their expression levels and patterns differ: ARGAH1 encoded the minor activity, and ARGAH1-driven β-glucuronidase (GUS) was expressed throughout the seedling; the ARGAH2::GUS expression pattern was more localized. Naphthaleneacetic acid increased seedling lateral root numbers (total lateral roots per primary root) in the mutants to twice the number in the wild type, consistent with increased internal NO leading to enhanced auxin signaling in roots. In agreement, argah1-1 and argah2-1 showed increased expression of the auxin-responsive reporter DR5::GUS in root tips, emerging lateral roots, and hypocotyls. We propose that Arg, or an Arg derivative, is a potential NO source and that reduced arginase activity in the mutants results in greater conversion of Arg to NO, thereby potentiating auxin action in roots. This model is supported by supplemental Arg induction of adventitious roots and increased NO accumulation in argah1-1 and argah2-1 versus the wild type.Fil: Flores, Teresita. University of Missouri; Estados UnidosFil: Todd, Christopher. University of Saskatchewan; Canadá. University of Missouri; Estados UnidosFil: Tovar Mendez, Alejandro. University of Missouri; Estados UnidosFil: Dhanoa, Preetinder. University of Guelph; CanadáFil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hoyos, Mary Elizabeth. University of Missouri; Estados UnidosFil: Brownfield, Disa M.. University of Alberta; CanadáFil: Mullen, Robert T.. University of Guelph; CanadáFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Polacco, Joe. Universidade Federal do Rio Grande do Sul; BrasilAmerican Society of Plant Biologist2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133922Flores, Teresita; Todd, Christopher; Tovar Mendez, Alejandro; Dhanoa, Preetinder; Correa Aragunde, Maria Natalia; et al.; Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development; American Society of Plant Biologist; Plant Physiology; 147; 4; 8-2008; 1936-19460032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.108.121459info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:22Zoai:ri.conicet.gov.ar:11336/133922instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:22.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
title Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
spellingShingle Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
Flores, Teresita
arginase
nitric oxide
roots
nitric oxide synthase
title_short Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
title_full Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
title_fullStr Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
title_full_unstemmed Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
title_sort Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development
dc.creator.none.fl_str_mv Flores, Teresita
Todd, Christopher
Tovar Mendez, Alejandro
Dhanoa, Preetinder
Correa Aragunde, Maria Natalia
Hoyos, Mary Elizabeth
Brownfield, Disa M.
Mullen, Robert T.
Lamattina, Lorenzo
Polacco, Joe
author Flores, Teresita
author_facet Flores, Teresita
Todd, Christopher
Tovar Mendez, Alejandro
Dhanoa, Preetinder
Correa Aragunde, Maria Natalia
Hoyos, Mary Elizabeth
Brownfield, Disa M.
Mullen, Robert T.
Lamattina, Lorenzo
Polacco, Joe
author_role author
author2 Todd, Christopher
Tovar Mendez, Alejandro
Dhanoa, Preetinder
Correa Aragunde, Maria Natalia
Hoyos, Mary Elizabeth
Brownfield, Disa M.
Mullen, Robert T.
Lamattina, Lorenzo
Polacco, Joe
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv arginase
nitric oxide
roots
nitric oxide synthase
topic arginase
nitric oxide
roots
nitric oxide synthase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mutation of either arginase structural gene (ARGAH1 or ARGAH2 encoding arginine [Arg] amidohydrolase-1 and -2, respectively) resulted in increased formation of lateral and adventitious roots in Arabidopsis (Arabidopsis thaliana) seedlings and increased nitric oxide (NO) accumulation and efflux, detected by the fluorogenic traps 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate and diamino-rhodamine-4M, respectively. Upon seedling exposure to the synthetic auxin naphthaleneacetic acid, NO accumulation was differentially enhanced in argah1-1 and argah2-1 compared with the wild type. In all genotypes, much 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate fluorescence originated from mitochondria. The arginases are both localized to the mitochondrial matrix and closely related. However, their expression levels and patterns differ: ARGAH1 encoded the minor activity, and ARGAH1-driven β-glucuronidase (GUS) was expressed throughout the seedling; the ARGAH2::GUS expression pattern was more localized. Naphthaleneacetic acid increased seedling lateral root numbers (total lateral roots per primary root) in the mutants to twice the number in the wild type, consistent with increased internal NO leading to enhanced auxin signaling in roots. In agreement, argah1-1 and argah2-1 showed increased expression of the auxin-responsive reporter DR5::GUS in root tips, emerging lateral roots, and hypocotyls. We propose that Arg, or an Arg derivative, is a potential NO source and that reduced arginase activity in the mutants results in greater conversion of Arg to NO, thereby potentiating auxin action in roots. This model is supported by supplemental Arg induction of adventitious roots and increased NO accumulation in argah1-1 and argah2-1 versus the wild type.
Fil: Flores, Teresita. University of Missouri; Estados Unidos
Fil: Todd, Christopher. University of Saskatchewan; Canadá. University of Missouri; Estados Unidos
Fil: Tovar Mendez, Alejandro. University of Missouri; Estados Unidos
Fil: Dhanoa, Preetinder. University of Guelph; Canadá
Fil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hoyos, Mary Elizabeth. University of Missouri; Estados Unidos
Fil: Brownfield, Disa M.. University of Alberta; Canadá
Fil: Mullen, Robert T.. University of Guelph; Canadá
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Polacco, Joe. Universidade Federal do Rio Grande do Sul; Brasil
description Mutation of either arginase structural gene (ARGAH1 or ARGAH2 encoding arginine [Arg] amidohydrolase-1 and -2, respectively) resulted in increased formation of lateral and adventitious roots in Arabidopsis (Arabidopsis thaliana) seedlings and increased nitric oxide (NO) accumulation and efflux, detected by the fluorogenic traps 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate and diamino-rhodamine-4M, respectively. Upon seedling exposure to the synthetic auxin naphthaleneacetic acid, NO accumulation was differentially enhanced in argah1-1 and argah2-1 compared with the wild type. In all genotypes, much 3-amino,4-aminomethyl-2′,7′- difluorofluorescein diacetate fluorescence originated from mitochondria. The arginases are both localized to the mitochondrial matrix and closely related. However, their expression levels and patterns differ: ARGAH1 encoded the minor activity, and ARGAH1-driven β-glucuronidase (GUS) was expressed throughout the seedling; the ARGAH2::GUS expression pattern was more localized. Naphthaleneacetic acid increased seedling lateral root numbers (total lateral roots per primary root) in the mutants to twice the number in the wild type, consistent with increased internal NO leading to enhanced auxin signaling in roots. In agreement, argah1-1 and argah2-1 showed increased expression of the auxin-responsive reporter DR5::GUS in root tips, emerging lateral roots, and hypocotyls. We propose that Arg, or an Arg derivative, is a potential NO source and that reduced arginase activity in the mutants results in greater conversion of Arg to NO, thereby potentiating auxin action in roots. This model is supported by supplemental Arg induction of adventitious roots and increased NO accumulation in argah1-1 and argah2-1 versus the wild type.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133922
Flores, Teresita; Todd, Christopher; Tovar Mendez, Alejandro; Dhanoa, Preetinder; Correa Aragunde, Maria Natalia; et al.; Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development; American Society of Plant Biologist; Plant Physiology; 147; 4; 8-2008; 1936-1946
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133922
identifier_str_mv Flores, Teresita; Todd, Christopher; Tovar Mendez, Alejandro; Dhanoa, Preetinder; Correa Aragunde, Maria Natalia; et al.; Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development; American Society of Plant Biologist; Plant Physiology; 147; 4; 8-2008; 1936-1946
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.108.121459
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268971062001664
score 13.13397

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