Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary

Autores
Velardez, Miguel Omar; de Laurentiis, Andrea; Díaz, María del Carmen; Lasaga, Mercedes Isabel; Pisera, Daniel Alberto; Seilicovich, Adriana; Duvilanski, Beatriz Haydee
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: In order to determine the mechanism by which nitric oxide (NO) inhibits prolactin release, we investigated the participation of cGMP-dependent cAMP-phosphodiesterases (PDEs) and protein kinase G (PKG) in this effect of NO. Methods: Anterior pituitary glands of male rats were incubated with inhibitors of PDE and PKG with or without sodium nitroprusside (NP). Prolactin release, and cAMP and cGMP concentrations were determined by RIA. Results and conclusions: The inhibitory effect of NP (0.5 mmol/l) on prolactin release and cAMP concentration was blocked by EHNA (10±4 mol/l) and HL-725 (10±4 mol/l), inhibitors of cGMPstimulated cAMP-PDE (PDE2). 8-Br-cGMP (10±4 and 10±3 mol/l), which mimics cGMP as a mediator of NP effects on prolactin release, also decreased cAMP concentration. Zaprinast (10±4 mol/l), a selective inhibitor of speci®c cGMP-PDE (PDE5), potentiated the NP effect on cAMP concentration. Rp-8-[(4-chlorophenyl)thio]-cGMP triethylamine (Rp-8-cGMP, 10±7±10±6 mol/l), an inhibitor of PKG, reversed the effect of NP on prolactin release. The present study suggests that several mechanisms are involved in the inhibitory effect of NO on prolactin release. The activation of PDE2 by cGMP may mediate the inhibitory effect of NO on cAMP concentration and therefore on prolactin release. NO-activated PKG may also be participating in the inhibitory effect of NO on prolactin release.
Fil: Velardez, Miguel Omar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Díaz, María del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Pisera, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Materia
Protein Kinase G
Nitric Oxide
Prolactin
Pituitary
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44718
Acceder
id CONICETDig_ae1f0c9f1915e4d296455dbaffad9615
oai_identifier_str oai:ri.conicet.gov.ar:11336/44718
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitaryVelardez, Miguel Omarde Laurentiis, AndreaDíaz, María del CarmenLasaga, Mercedes IsabelPisera, Daniel AlbertoSeilicovich, AdrianaDuvilanski, Beatriz HaydeeProtein Kinase GNitric OxideProlactinPituitaryhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: In order to determine the mechanism by which nitric oxide (NO) inhibits prolactin release, we investigated the participation of cGMP-dependent cAMP-phosphodiesterases (PDEs) and protein kinase G (PKG) in this effect of NO. Methods: Anterior pituitary glands of male rats were incubated with inhibitors of PDE and PKG with or without sodium nitroprusside (NP). Prolactin release, and cAMP and cGMP concentrations were determined by RIA. Results and conclusions: The inhibitory effect of NP (0.5 mmol/l) on prolactin release and cAMP concentration was blocked by EHNA (10±4 mol/l) and HL-725 (10±4 mol/l), inhibitors of cGMPstimulated cAMP-PDE (PDE2). 8-Br-cGMP (10±4 and 10±3 mol/l), which mimics cGMP as a mediator of NP effects on prolactin release, also decreased cAMP concentration. Zaprinast (10±4 mol/l), a selective inhibitor of speci®c cGMP-PDE (PDE5), potentiated the NP effect on cAMP concentration. Rp-8-[(4-chlorophenyl)thio]-cGMP triethylamine (Rp-8-cGMP, 10±7±10±6 mol/l), an inhibitor of PKG, reversed the effect of NP on prolactin release. The present study suggests that several mechanisms are involved in the inhibitory effect of NO on prolactin release. The activation of PDE2 by cGMP may mediate the inhibitory effect of NO on cAMP concentration and therefore on prolactin release. NO-activated PKG may also be participating in the inhibitory effect of NO on prolactin release.Fil: Velardez, Miguel Omar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Díaz, María del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Pisera, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaBioScientifica2000-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44718Velardez, Miguel Omar; de Laurentiis, Andrea; Díaz, María del Carmen; Lasaga, Mercedes Isabel; Pisera, Daniel Alberto; et al.; Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary; BioScientifica; European Journal of Endocrinology; 143; 2; 8-2000; 279-2840804-4643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.eje-online.org/content/143/2/279.full.pdf+htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:17Zoai:ri.conicet.gov.ar:11336/44718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:17.472CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
title Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
spellingShingle Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
Velardez, Miguel Omar
Protein Kinase G
Nitric Oxide
Prolactin
Pituitary
title_short Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
title_full Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
title_fullStr Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
title_full_unstemmed Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
title_sort Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary
dc.creator.none.fl_str_mv Velardez, Miguel Omar
de Laurentiis, Andrea
Díaz, María del Carmen
Lasaga, Mercedes Isabel
Pisera, Daniel Alberto
Seilicovich, Adriana
Duvilanski, Beatriz Haydee
author Velardez, Miguel Omar
author_facet Velardez, Miguel Omar
de Laurentiis, Andrea
Díaz, María del Carmen
Lasaga, Mercedes Isabel
Pisera, Daniel Alberto
Seilicovich, Adriana
Duvilanski, Beatriz Haydee
author_role author
author2 de Laurentiis, Andrea
Díaz, María del Carmen
Lasaga, Mercedes Isabel
Pisera, Daniel Alberto
Seilicovich, Adriana
Duvilanski, Beatriz Haydee
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Protein Kinase G
Nitric Oxide
Prolactin
Pituitary
topic Protein Kinase G
Nitric Oxide
Prolactin
Pituitary
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: In order to determine the mechanism by which nitric oxide (NO) inhibits prolactin release, we investigated the participation of cGMP-dependent cAMP-phosphodiesterases (PDEs) and protein kinase G (PKG) in this effect of NO. Methods: Anterior pituitary glands of male rats were incubated with inhibitors of PDE and PKG with or without sodium nitroprusside (NP). Prolactin release, and cAMP and cGMP concentrations were determined by RIA. Results and conclusions: The inhibitory effect of NP (0.5 mmol/l) on prolactin release and cAMP concentration was blocked by EHNA (10±4 mol/l) and HL-725 (10±4 mol/l), inhibitors of cGMPstimulated cAMP-PDE (PDE2). 8-Br-cGMP (10±4 and 10±3 mol/l), which mimics cGMP as a mediator of NP effects on prolactin release, also decreased cAMP concentration. Zaprinast (10±4 mol/l), a selective inhibitor of speci®c cGMP-PDE (PDE5), potentiated the NP effect on cAMP concentration. Rp-8-[(4-chlorophenyl)thio]-cGMP triethylamine (Rp-8-cGMP, 10±7±10±6 mol/l), an inhibitor of PKG, reversed the effect of NP on prolactin release. The present study suggests that several mechanisms are involved in the inhibitory effect of NO on prolactin release. The activation of PDE2 by cGMP may mediate the inhibitory effect of NO on cAMP concentration and therefore on prolactin release. NO-activated PKG may also be participating in the inhibitory effect of NO on prolactin release.
Fil: Velardez, Miguel Omar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: de Laurentiis, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Díaz, María del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Pisera, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
description Objective: In order to determine the mechanism by which nitric oxide (NO) inhibits prolactin release, we investigated the participation of cGMP-dependent cAMP-phosphodiesterases (PDEs) and protein kinase G (PKG) in this effect of NO. Methods: Anterior pituitary glands of male rats were incubated with inhibitors of PDE and PKG with or without sodium nitroprusside (NP). Prolactin release, and cAMP and cGMP concentrations were determined by RIA. Results and conclusions: The inhibitory effect of NP (0.5 mmol/l) on prolactin release and cAMP concentration was blocked by EHNA (10±4 mol/l) and HL-725 (10±4 mol/l), inhibitors of cGMPstimulated cAMP-PDE (PDE2). 8-Br-cGMP (10±4 and 10±3 mol/l), which mimics cGMP as a mediator of NP effects on prolactin release, also decreased cAMP concentration. Zaprinast (10±4 mol/l), a selective inhibitor of speci®c cGMP-PDE (PDE5), potentiated the NP effect on cAMP concentration. Rp-8-[(4-chlorophenyl)thio]-cGMP triethylamine (Rp-8-cGMP, 10±7±10±6 mol/l), an inhibitor of PKG, reversed the effect of NP on prolactin release. The present study suggests that several mechanisms are involved in the inhibitory effect of NO on prolactin release. The activation of PDE2 by cGMP may mediate the inhibitory effect of NO on cAMP concentration and therefore on prolactin release. NO-activated PKG may also be participating in the inhibitory effect of NO on prolactin release.
publishDate 2000
dc.date.none.fl_str_mv 2000-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44718
Velardez, Miguel Omar; de Laurentiis, Andrea; Díaz, María del Carmen; Lasaga, Mercedes Isabel; Pisera, Daniel Alberto; et al.; Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary; BioScientifica; European Journal of Endocrinology; 143; 2; 8-2000; 279-284
0804-4643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44718
identifier_str_mv Velardez, Miguel Omar; de Laurentiis, Andrea; Díaz, María del Carmen; Lasaga, Mercedes Isabel; Pisera, Daniel Alberto; et al.; Role of phosphidiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary; BioScientifica; European Journal of Endocrinology; 143; 2; 8-2000; 279-284
0804-4643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eje-online.org/content/143/2/279.full.pdf+html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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