Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices

Autores
Hassan, Natalia; Verdinelli, Valeria; Ruso, Juan M.; Messina, Paula Verónica
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An enhanced knowledge of the interaction of proteins with the surfaces of implantable materials, particularly regarding fibrinogen (Fb), is fundamental for understanding cellular events and the overall host response. Thinking of future use of Si-nanofibers as three-dimensional (3D) scaffolds for construction of implantable artificial devices, the correlation among the material surface characteristics and the amount, structure and distribution of adsorbed Fb molecules are analyzed. The Fb adsorption process occurs in a stepwise fashion with an initial rapid adsorption, an intermediate reorganization and finally a second slower adsorption regime over a longer period of time. There is a partial desorption of the protein after the first adsorption process, which demonstrates that this step is reversible until 2 × 104 s. Nevertheless the whole process is irreversible, with a high distortion of the original material morphology. The limiting value for the adsorbed Fb surface concentration is about 270 ± 20 μg dm-2; more than three times the adsorption capacity of non fibrillar, 2D or 3D, scaffolds. The fibrous structure and the similitude in size between the substrate (d = 30-50 nm) and the Fb molecules (47-50 nm) are proposed to be the key to the enhanced adsorption process and the acquired final topography of the material.
Fil: Hassan, Natalia. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Verdinelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
Fibrinogen
Adsorption
Sio2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63306
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devicesHassan, NataliaVerdinelli, ValeriaRuso, Juan M.Messina, Paula VerónicaFibrinogenAdsorptionSio2https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2An enhanced knowledge of the interaction of proteins with the surfaces of implantable materials, particularly regarding fibrinogen (Fb), is fundamental for understanding cellular events and the overall host response. Thinking of future use of Si-nanofibers as three-dimensional (3D) scaffolds for construction of implantable artificial devices, the correlation among the material surface characteristics and the amount, structure and distribution of adsorbed Fb molecules are analyzed. The Fb adsorption process occurs in a stepwise fashion with an initial rapid adsorption, an intermediate reorganization and finally a second slower adsorption regime over a longer period of time. There is a partial desorption of the protein after the first adsorption process, which demonstrates that this step is reversible until 2 × 104 s. Nevertheless the whole process is irreversible, with a high distortion of the original material morphology. The limiting value for the adsorbed Fb surface concentration is about 270 ± 20 μg dm-2; more than three times the adsorption capacity of non fibrillar, 2D or 3D, scaffolds. The fibrous structure and the similitude in size between the substrate (d = 30-50 nm) and the Fb molecules (47-50 nm) are proposed to be the key to the enhanced adsorption process and the acquired final topography of the material.Fil: Hassan, Natalia. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Verdinelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; EspañaFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaRoyal Society of Chemistry2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63306Hassan, Natalia; Verdinelli, Valeria; Ruso, Juan M.; Messina, Paula Verónica; Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices; Royal Society of Chemistry; Soft Matter; 8; 24; 6-2012; 6582-65921744-683XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm25489ainfo:eu-repo/semantics/altIdentifier/doi/10.1039/c2sm25489ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:44Zoai:ri.conicet.gov.ar:11336/63306instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:45.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
title Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
spellingShingle Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
Hassan, Natalia
Fibrinogen
Adsorption
Sio2
title_short Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
title_full Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
title_fullStr Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
title_full_unstemmed Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
title_sort Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices
dc.creator.none.fl_str_mv Hassan, Natalia
Verdinelli, Valeria
Ruso, Juan M.
Messina, Paula Verónica
author Hassan, Natalia
author_facet Hassan, Natalia
Verdinelli, Valeria
Ruso, Juan M.
Messina, Paula Verónica
author_role author
author2 Verdinelli, Valeria
Ruso, Juan M.
Messina, Paula Verónica
author2_role author
author
author
dc.subject.none.fl_str_mv Fibrinogen
Adsorption
Sio2
topic Fibrinogen
Adsorption
Sio2
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv An enhanced knowledge of the interaction of proteins with the surfaces of implantable materials, particularly regarding fibrinogen (Fb), is fundamental for understanding cellular events and the overall host response. Thinking of future use of Si-nanofibers as three-dimensional (3D) scaffolds for construction of implantable artificial devices, the correlation among the material surface characteristics and the amount, structure and distribution of adsorbed Fb molecules are analyzed. The Fb adsorption process occurs in a stepwise fashion with an initial rapid adsorption, an intermediate reorganization and finally a second slower adsorption regime over a longer period of time. There is a partial desorption of the protein after the first adsorption process, which demonstrates that this step is reversible until 2 × 104 s. Nevertheless the whole process is irreversible, with a high distortion of the original material morphology. The limiting value for the adsorbed Fb surface concentration is about 270 ± 20 μg dm-2; more than three times the adsorption capacity of non fibrillar, 2D or 3D, scaffolds. The fibrous structure and the similitude in size between the substrate (d = 30-50 nm) and the Fb molecules (47-50 nm) are proposed to be the key to the enhanced adsorption process and the acquired final topography of the material.
Fil: Hassan, Natalia. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Verdinelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela. Facultad de Física; España
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description An enhanced knowledge of the interaction of proteins with the surfaces of implantable materials, particularly regarding fibrinogen (Fb), is fundamental for understanding cellular events and the overall host response. Thinking of future use of Si-nanofibers as three-dimensional (3D) scaffolds for construction of implantable artificial devices, the correlation among the material surface characteristics and the amount, structure and distribution of adsorbed Fb molecules are analyzed. The Fb adsorption process occurs in a stepwise fashion with an initial rapid adsorption, an intermediate reorganization and finally a second slower adsorption regime over a longer period of time. There is a partial desorption of the protein after the first adsorption process, which demonstrates that this step is reversible until 2 × 104 s. Nevertheless the whole process is irreversible, with a high distortion of the original material morphology. The limiting value for the adsorbed Fb surface concentration is about 270 ± 20 μg dm-2; more than three times the adsorption capacity of non fibrillar, 2D or 3D, scaffolds. The fibrous structure and the similitude in size between the substrate (d = 30-50 nm) and the Fb molecules (47-50 nm) are proposed to be the key to the enhanced adsorption process and the acquired final topography of the material.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63306
Hassan, Natalia; Verdinelli, Valeria; Ruso, Juan M.; Messina, Paula Verónica; Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices; Royal Society of Chemistry; Soft Matter; 8; 24; 6-2012; 6582-6592
1744-683X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63306
identifier_str_mv Hassan, Natalia; Verdinelli, Valeria; Ruso, Juan M.; Messina, Paula Verónica; Assessing structure and dynamics of fibrinogen films on silicon nanofibers: Towards hemocompatibility devices; Royal Society of Chemistry; Soft Matter; 8; 24; 6-2012; 6582-6592
1744-683X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm25489a
info:eu-repo/semantics/altIdentifier/doi/10.1039/c2sm25489a
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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