Lonomia obliqua venom action on fibrinolytic system

Autores
Fritzen, Márcio; Schattner, Mirta Ana; Quintana Ribeiro, Ana Laura; Correia Batista, Isabel De Fátima; Ventura, Janaina; Carlos Prezoto, Benedito; Chudzinski-Tavassi, Ana Marisa
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Accidental skin contact with the Lonomia caterpillar bristles causes a severe hemorrhagic syndrome. While fibrinolytic activation is considered to be the main cause of hemorrhage in Lonomia achelous envenomation, a consumptive coagulopathy was found to be a major component involved in the bleeding complications observed in patients envenomed by contact with Lonomia obliqua. Although we have previously observed that in L. obliqua envenomations, fibrinolysis activation appeared to be secondary to coagulation system activation, there are no reports regarding the ability of L. obliqua venom to activate directly fibrinolytic pathways. We examined the action of L. obliqua crude bristles extract (LOCBE) on several fibrinolytic system components. We demonstrated that LOCBE degraded the A-alpha fibrinogen chain only at high concentrations and after long incubation times. Under these conditions, LOCBE also induced prolongation of the fibrinogen clotting time, but no clot lysis was observed before 24 h. LOCBE did not contain t-PA- or u-PA-like activities. Gel filtration and SDS-PAGE showed that LOCBE did not induce FXIII digestion. In addition, no FXIII activity inhibition was detected by dansylcadaverin method. FXIII levels remained unchanged when FXIII was measured in fibrinogen-depleted LOCBE-treated rat plasma, suggesting that the observed 50% FXIII reduction in rats was related to consumption. In conclusion, our results clearly demonstrated that LOCBE did not display either FXIII inhibition or degradation nor fibrinolytic activity. Furthermore, although proteolytic activity on Aα fibrinogen chain was observed, cross-linked fibrin was not affected by LOCBE. © 2003 Elsevier Ltd. All rights reserved.
Fil: Fritzen, Márcio. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Quintana Ribeiro, Ana Laura. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Correia Batista, Isabel De Fátima. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Ventura, Janaina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Carlos Prezoto, Benedito. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Chudzinski-Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Materia
Caterpillar
Envenomation
Factor Xiii
Fibrinogen
Fibrinolysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66450
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66450
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lonomia obliqua venom action on fibrinolytic systemFritzen, MárcioSchattner, Mirta AnaQuintana Ribeiro, Ana LauraCorreia Batista, Isabel De FátimaVentura, JanainaCarlos Prezoto, BeneditoChudzinski-Tavassi, Ana MarisaCaterpillarEnvenomationFactor XiiiFibrinogenFibrinolysishttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Accidental skin contact with the Lonomia caterpillar bristles causes a severe hemorrhagic syndrome. While fibrinolytic activation is considered to be the main cause of hemorrhage in Lonomia achelous envenomation, a consumptive coagulopathy was found to be a major component involved in the bleeding complications observed in patients envenomed by contact with Lonomia obliqua. Although we have previously observed that in L. obliqua envenomations, fibrinolysis activation appeared to be secondary to coagulation system activation, there are no reports regarding the ability of L. obliqua venom to activate directly fibrinolytic pathways. We examined the action of L. obliqua crude bristles extract (LOCBE) on several fibrinolytic system components. We demonstrated that LOCBE degraded the A-alpha fibrinogen chain only at high concentrations and after long incubation times. Under these conditions, LOCBE also induced prolongation of the fibrinogen clotting time, but no clot lysis was observed before 24 h. LOCBE did not contain t-PA- or u-PA-like activities. Gel filtration and SDS-PAGE showed that LOCBE did not induce FXIII digestion. In addition, no FXIII activity inhibition was detected by dansylcadaverin method. FXIII levels remained unchanged when FXIII was measured in fibrinogen-depleted LOCBE-treated rat plasma, suggesting that the observed 50% FXIII reduction in rats was related to consumption. In conclusion, our results clearly demonstrated that LOCBE did not display either FXIII inhibition or degradation nor fibrinolytic activity. Furthermore, although proteolytic activity on Aα fibrinogen chain was observed, cross-linked fibrin was not affected by LOCBE. © 2003 Elsevier Ltd. All rights reserved.Fil: Fritzen, Márcio. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Quintana Ribeiro, Ana Laura. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Correia Batista, Isabel De Fátima. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Ventura, Janaina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Carlos Prezoto, Benedito. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Chudzinski-Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilPergamon-Elsevier Science Ltd2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66450Fritzen, Márcio; Schattner, Mirta Ana; Quintana Ribeiro, Ana Laura; Correia Batista, Isabel De Fátima; Ventura, Janaina; et al.; Lonomia obliqua venom action on fibrinolytic system; Pergamon-Elsevier Science Ltd; Thrombosis Research; 112; 1-2; 12-2003; 105-1100049-3848CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0049384803005863info:eu-repo/semantics/altIdentifier/doi/10.1016/j.thromres.2003.11.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:43Zoai:ri.conicet.gov.ar:11336/66450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:44.145CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lonomia obliqua venom action on fibrinolytic system
title Lonomia obliqua venom action on fibrinolytic system
spellingShingle Lonomia obliqua venom action on fibrinolytic system
Fritzen, Márcio
Caterpillar
Envenomation
Factor Xiii
Fibrinogen
Fibrinolysis
title_short Lonomia obliqua venom action on fibrinolytic system
title_full Lonomia obliqua venom action on fibrinolytic system
title_fullStr Lonomia obliqua venom action on fibrinolytic system
title_full_unstemmed Lonomia obliqua venom action on fibrinolytic system
title_sort Lonomia obliqua venom action on fibrinolytic system
dc.creator.none.fl_str_mv Fritzen, Márcio
Schattner, Mirta Ana
Quintana Ribeiro, Ana Laura
Correia Batista, Isabel De Fátima
Ventura, Janaina
Carlos Prezoto, Benedito
Chudzinski-Tavassi, Ana Marisa
author Fritzen, Márcio
author_facet Fritzen, Márcio
Schattner, Mirta Ana
Quintana Ribeiro, Ana Laura
Correia Batista, Isabel De Fátima
Ventura, Janaina
Carlos Prezoto, Benedito
Chudzinski-Tavassi, Ana Marisa
author_role author
author2 Schattner, Mirta Ana
Quintana Ribeiro, Ana Laura
Correia Batista, Isabel De Fátima
Ventura, Janaina
Carlos Prezoto, Benedito
Chudzinski-Tavassi, Ana Marisa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Caterpillar
Envenomation
Factor Xiii
Fibrinogen
Fibrinolysis
topic Caterpillar
Envenomation
Factor Xiii
Fibrinogen
Fibrinolysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Accidental skin contact with the Lonomia caterpillar bristles causes a severe hemorrhagic syndrome. While fibrinolytic activation is considered to be the main cause of hemorrhage in Lonomia achelous envenomation, a consumptive coagulopathy was found to be a major component involved in the bleeding complications observed in patients envenomed by contact with Lonomia obliqua. Although we have previously observed that in L. obliqua envenomations, fibrinolysis activation appeared to be secondary to coagulation system activation, there are no reports regarding the ability of L. obliqua venom to activate directly fibrinolytic pathways. We examined the action of L. obliqua crude bristles extract (LOCBE) on several fibrinolytic system components. We demonstrated that LOCBE degraded the A-alpha fibrinogen chain only at high concentrations and after long incubation times. Under these conditions, LOCBE also induced prolongation of the fibrinogen clotting time, but no clot lysis was observed before 24 h. LOCBE did not contain t-PA- or u-PA-like activities. Gel filtration and SDS-PAGE showed that LOCBE did not induce FXIII digestion. In addition, no FXIII activity inhibition was detected by dansylcadaverin method. FXIII levels remained unchanged when FXIII was measured in fibrinogen-depleted LOCBE-treated rat plasma, suggesting that the observed 50% FXIII reduction in rats was related to consumption. In conclusion, our results clearly demonstrated that LOCBE did not display either FXIII inhibition or degradation nor fibrinolytic activity. Furthermore, although proteolytic activity on Aα fibrinogen chain was observed, cross-linked fibrin was not affected by LOCBE. © 2003 Elsevier Ltd. All rights reserved.
Fil: Fritzen, Márcio. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Quintana Ribeiro, Ana Laura. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Correia Batista, Isabel De Fátima. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Ventura, Janaina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Carlos Prezoto, Benedito. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Chudzinski-Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
description Accidental skin contact with the Lonomia caterpillar bristles causes a severe hemorrhagic syndrome. While fibrinolytic activation is considered to be the main cause of hemorrhage in Lonomia achelous envenomation, a consumptive coagulopathy was found to be a major component involved in the bleeding complications observed in patients envenomed by contact with Lonomia obliqua. Although we have previously observed that in L. obliqua envenomations, fibrinolysis activation appeared to be secondary to coagulation system activation, there are no reports regarding the ability of L. obliqua venom to activate directly fibrinolytic pathways. We examined the action of L. obliqua crude bristles extract (LOCBE) on several fibrinolytic system components. We demonstrated that LOCBE degraded the A-alpha fibrinogen chain only at high concentrations and after long incubation times. Under these conditions, LOCBE also induced prolongation of the fibrinogen clotting time, but no clot lysis was observed before 24 h. LOCBE did not contain t-PA- or u-PA-like activities. Gel filtration and SDS-PAGE showed that LOCBE did not induce FXIII digestion. In addition, no FXIII activity inhibition was detected by dansylcadaverin method. FXIII levels remained unchanged when FXIII was measured in fibrinogen-depleted LOCBE-treated rat plasma, suggesting that the observed 50% FXIII reduction in rats was related to consumption. In conclusion, our results clearly demonstrated that LOCBE did not display either FXIII inhibition or degradation nor fibrinolytic activity. Furthermore, although proteolytic activity on Aα fibrinogen chain was observed, cross-linked fibrin was not affected by LOCBE. © 2003 Elsevier Ltd. All rights reserved.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66450
Fritzen, Márcio; Schattner, Mirta Ana; Quintana Ribeiro, Ana Laura; Correia Batista, Isabel De Fátima; Ventura, Janaina; et al.; Lonomia obliqua venom action on fibrinolytic system; Pergamon-Elsevier Science Ltd; Thrombosis Research; 112; 1-2; 12-2003; 105-110
0049-3848
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66450
identifier_str_mv Fritzen, Márcio; Schattner, Mirta Ana; Quintana Ribeiro, Ana Laura; Correia Batista, Isabel De Fátima; Ventura, Janaina; et al.; Lonomia obliqua venom action on fibrinolytic system; Pergamon-Elsevier Science Ltd; Thrombosis Research; 112; 1-2; 12-2003; 105-110
0049-3848
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0049384803005863
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.thromres.2003.11.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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