Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process

Autores
Fanovich, Maria Alejandra; Ivanovic, J.; Misic, D.; Alvarez, María Victoria; Jaeger, P.; Zizovic, I.; Eggers, Rudolf
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 °C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 °C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; Argentina
Fil: Ivanovic, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina
Fil: Misic, D.. University of Belgrade; Serbia
Fil: Alvarez, María Victoria. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; Argentina
Fil: Jaeger, P.. Universitat Hamburg; Alemania
Fil: Zizovic, I.. University of Belgrade; Serbia
Fil: Eggers, Rudolf. Universitat Hamburg; Alemania
Materia
ANTIBACTERIAL PROPERTIES
POLYCAPROLACTONE SCAFFOLD
SUPERCRITICAL EXTRACTION IMPREGNATION
USNEA EXTRACT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2440
Acceder
id CONICETDig_002d8821074caddeeeafa44cd69d1eda
oai_identifier_str oai:ri.conicet.gov.ar:11336/2440
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation processFanovich, Maria AlejandraIvanovic, J.Misic, D.Alvarez, María VictoriaJaeger, P.Zizovic, I.Eggers, RudolfANTIBACTERIAL PROPERTIESPOLYCAPROLACTONE SCAFFOLDSUPERCRITICAL EXTRACTION IMPREGNATIONUSNEA EXTRACThttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 °C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 °C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; ArgentinaFil: Ivanovic, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); ArgentinaFil: Misic, D.. University of Belgrade; SerbiaFil: Alvarez, María Victoria. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; ArgentinaFil: Jaeger, P.. Universitat Hamburg; AlemaniaFil: Zizovic, I.. University of Belgrade; SerbiaFil: Eggers, Rudolf. Universitat Hamburg; AlemaniaElsevier Science2013-03-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2440Fanovich, Maria Alejandra; Ivanovic, J.; Misic, D.; Alvarez, María Victoria; Jaeger, P.; et al.; Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process; Elsevier Science; Journal of Supercritical Fluids; 78; 23-3-2013; 42-530896-8446enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.supflu.2013.03.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0896844613001150info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:32Zoai:ri.conicet.gov.ar:11336/2440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:32.463CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
title Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
spellingShingle Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
Fanovich, Maria Alejandra
ANTIBACTERIAL PROPERTIES
POLYCAPROLACTONE SCAFFOLD
SUPERCRITICAL EXTRACTION IMPREGNATION
USNEA EXTRACT
title_short Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
title_full Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
title_fullStr Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
title_full_unstemmed Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
title_sort Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
dc.creator.none.fl_str_mv Fanovich, Maria Alejandra
Ivanovic, J.
Misic, D.
Alvarez, María Victoria
Jaeger, P.
Zizovic, I.
Eggers, Rudolf
author Fanovich, Maria Alejandra
author_facet Fanovich, Maria Alejandra
Ivanovic, J.
Misic, D.
Alvarez, María Victoria
Jaeger, P.
Zizovic, I.
Eggers, Rudolf
author_role author
author2 Ivanovic, J.
Misic, D.
Alvarez, María Victoria
Jaeger, P.
Zizovic, I.
Eggers, Rudolf
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBACTERIAL PROPERTIES
POLYCAPROLACTONE SCAFFOLD
SUPERCRITICAL EXTRACTION IMPREGNATION
USNEA EXTRACT
topic ANTIBACTERIAL PROPERTIES
POLYCAPROLACTONE SCAFFOLD
SUPERCRITICAL EXTRACTION IMPREGNATION
USNEA EXTRACT
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 °C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 °C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; Argentina
Fil: Ivanovic, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); Argentina
Fil: Misic, D.. University of Belgrade; Serbia
Fil: Alvarez, María Victoria. Universidad Nacional de Mar del Plata. Facultad de Ingenieria; Argentina
Fil: Jaeger, P.. Universitat Hamburg; Alemania
Fil: Zizovic, I.. University of Belgrade; Serbia
Fil: Eggers, Rudolf. Universitat Hamburg; Alemania
description The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 °C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 °C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2440
Fanovich, Maria Alejandra; Ivanovic, J.; Misic, D.; Alvarez, María Victoria; Jaeger, P.; et al.; Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process; Elsevier Science; Journal of Supercritical Fluids; 78; 23-3-2013; 42-53
0896-8446
url http://hdl.handle.net/11336/2440
identifier_str_mv Fanovich, Maria Alejandra; Ivanovic, J.; Misic, D.; Alvarez, María Victoria; Jaeger, P.; et al.; Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process; Elsevier Science; Journal of Supercritical Fluids; 78; 23-3-2013; 42-53
0896-8446
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.supflu.2013.03.017
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0896844613001150
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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