Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae

Autores
Carbo, Natalia; Tarkowski, Nahuel; Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; Aguilar, Pablo Sebastián
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells experience a rise of cytosolic Ca2+ that is mediated by two import systems composed by the Mid1-Cch1-Ecm7 protein complex, and the Fig 1 protein. Although this response has been largely studied, there is no report on Ca2+ dynamics at the single cell level. Here, using protein calcium indicators we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 secs, span between 7 and 30 secs and decay fitting a single exponential model. In vegetative cells bursts are scarce but preferentially occur when cells are transitioning G1 and S phase. Upon pheromone presence Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig 1 and a third, still unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 experiences nuclear localization bursts during the pheromone response.
Fil: Carbo, Natalia. Instituto Pasteur de Montevideo; Uruguay
Fil: Tarkowski, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Perez Ipiña, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Aguilar, Pablo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
CALCIUM SIGNALS
YEAST
CALCIUM BURSTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49309

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network_name_str CONICET Digital (CONICET)
spelling Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiaeCarbo, NataliaTarkowski, NahuelPerez Ipiña, EmilianoPonce Dawson, Silvina MarthaAguilar, Pablo SebastiánCALCIUM SIGNALSYEASTCALCIUM BURSTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells experience a rise of cytosolic Ca2+ that is mediated by two import systems composed by the Mid1-Cch1-Ecm7 protein complex, and the Fig 1 protein. Although this response has been largely studied, there is no report on Ca2+ dynamics at the single cell level. Here, using protein calcium indicators we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 secs, span between 7 and 30 secs and decay fitting a single exponential model. In vegetative cells bursts are scarce but preferentially occur when cells are transitioning G1 and S phase. Upon pheromone presence Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig 1 and a third, still unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 experiences nuclear localization bursts during the pheromone response.Fil: Carbo, Natalia. Instituto Pasteur de Montevideo; UruguayFil: Tarkowski, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Perez Ipiña, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaFil: Aguilar, Pablo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaAmerican Society for Cell Biology2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49309Carbo, Natalia; Tarkowski, Nahuel; Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; Aguilar, Pablo Sebastián; Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae; American Society for Cell Biology; Molecular Biology Of The Cell; 28; 4; 12-2016; 501-5101059-1524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1091/mbc.e16-07-0481info:eu-repo/semantics/altIdentifier/url/https://www.molbiolcell.org/doi/10.1091/mbc.e16-07-0481info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305257/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:10Zoai:ri.conicet.gov.ar:11336/49309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:10.628CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
title Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
spellingShingle Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
Carbo, Natalia
CALCIUM SIGNALS
YEAST
CALCIUM BURSTS
title_short Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
title_full Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
title_fullStr Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
title_full_unstemmed Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
title_sort Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Carbo, Natalia
Tarkowski, Nahuel
Perez Ipiña, Emiliano
Ponce Dawson, Silvina Martha
Aguilar, Pablo Sebastián
author Carbo, Natalia
author_facet Carbo, Natalia
Tarkowski, Nahuel
Perez Ipiña, Emiliano
Ponce Dawson, Silvina Martha
Aguilar, Pablo Sebastián
author_role author
author2 Tarkowski, Nahuel
Perez Ipiña, Emiliano
Ponce Dawson, Silvina Martha
Aguilar, Pablo Sebastián
author2_role author
author
author
author
dc.subject.none.fl_str_mv CALCIUM SIGNALS
YEAST
CALCIUM BURSTS
topic CALCIUM SIGNALS
YEAST
CALCIUM BURSTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells experience a rise of cytosolic Ca2+ that is mediated by two import systems composed by the Mid1-Cch1-Ecm7 protein complex, and the Fig 1 protein. Although this response has been largely studied, there is no report on Ca2+ dynamics at the single cell level. Here, using protein calcium indicators we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 secs, span between 7 and 30 secs and decay fitting a single exponential model. In vegetative cells bursts are scarce but preferentially occur when cells are transitioning G1 and S phase. Upon pheromone presence Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig 1 and a third, still unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 experiences nuclear localization bursts during the pheromone response.
Fil: Carbo, Natalia. Instituto Pasteur de Montevideo; Uruguay
Fil: Tarkowski, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Perez Ipiña, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Aguilar, Pablo Sebastián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells experience a rise of cytosolic Ca2+ that is mediated by two import systems composed by the Mid1-Cch1-Ecm7 protein complex, and the Fig 1 protein. Although this response has been largely studied, there is no report on Ca2+ dynamics at the single cell level. Here, using protein calcium indicators we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 secs, span between 7 and 30 secs and decay fitting a single exponential model. In vegetative cells bursts are scarce but preferentially occur when cells are transitioning G1 and S phase. Upon pheromone presence Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig 1 and a third, still unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 experiences nuclear localization bursts during the pheromone response.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49309
Carbo, Natalia; Tarkowski, Nahuel; Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; Aguilar, Pablo Sebastián; Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae; American Society for Cell Biology; Molecular Biology Of The Cell; 28; 4; 12-2016; 501-510
1059-1524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49309
identifier_str_mv Carbo, Natalia; Tarkowski, Nahuel; Perez Ipiña, Emiliano; Ponce Dawson, Silvina Martha; Aguilar, Pablo Sebastián; Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae; American Society for Cell Biology; Molecular Biology Of The Cell; 28; 4; 12-2016; 501-510
1059-1524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1091/mbc.e16-07-0481
info:eu-repo/semantics/altIdentifier/url/https://www.molbiolcell.org/doi/10.1091/mbc.e16-07-0481
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305257/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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