Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs

Autores
Navarro, Francisco Ezequiel; Popovich, Cecilia; Sánchez, Miguel; Costilla, Ignacio; Gutierrez Ayesta, Cecilia; Leonardi, Patricia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.
Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina
Materia
Biología Celular, Microbiología
Diatomeas
Pared Celular
Reconstrucción tridimensional
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/11334

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network_name_str CIC Digital (CICBA)
spelling Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron MicrographsNavarro, Francisco EzequielPopovich, CeciliaSánchez, MiguelCostilla, IgnacioGutierrez Ayesta, CeciliaLeonardi, PatriciaBiología Celular, MicrobiologíaDiatomeasPared CelularReconstrucción tridimensionalThe diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina2020-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11334enginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927620001154info:eu-repo/semantics/altIdentifier/issn/1431-9276info:eu-repo/semantics/altIdentifier/issn/1435-8115info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:22Zoai:digital.cic.gba.gob.ar:11746/11334Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:23.124CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
title Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
spellingShingle Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
Navarro, Francisco Ezequiel
Biología Celular, Microbiología
Diatomeas
Pared Celular
Reconstrucción tridimensional
title_short Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
title_full Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
title_fullStr Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
title_full_unstemmed Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
title_sort Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
dc.creator.none.fl_str_mv Navarro, Francisco Ezequiel
Popovich, Cecilia
Sánchez, Miguel
Costilla, Ignacio
Gutierrez Ayesta, Cecilia
Leonardi, Patricia
author Navarro, Francisco Ezequiel
author_facet Navarro, Francisco Ezequiel
Popovich, Cecilia
Sánchez, Miguel
Costilla, Ignacio
Gutierrez Ayesta, Cecilia
Leonardi, Patricia
author_role author
author2 Popovich, Cecilia
Sánchez, Miguel
Costilla, Ignacio
Gutierrez Ayesta, Cecilia
Leonardi, Patricia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología Celular, Microbiología
Diatomeas
Pared Celular
Reconstrucción tridimensional
topic Biología Celular, Microbiología
Diatomeas
Pared Celular
Reconstrucción tridimensional
dc.description.none.fl_txt_mv The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.
Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina
description The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
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repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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