Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs
- Autores
- Navarro, Francisco Ezequiel; Popovich, Cecilia; Sánchez, Miguel; Costilla, Ignacio; Gutierrez Ayesta, Cecilia; Leonardi, Patricia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.
Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina - Materia
-
Biología Celular, Microbiología
Diatomeas
Pared Celular
Reconstrucción tridimensional - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/11334
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Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron MicrographsNavarro, Francisco EzequielPopovich, CeciliaSánchez, MiguelCostilla, IgnacioGutierrez Ayesta, CeciliaLeonardi, PatriciaBiología Celular, MicrobiologíaDiatomeasPared CelularReconstrucción tridimensionalThe diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications.Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina2020-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11334enginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927620001154info:eu-repo/semantics/altIdentifier/issn/1431-9276info:eu-repo/semantics/altIdentifier/issn/1435-8115info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:22Zoai:digital.cic.gba.gob.ar:11746/11334Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:23.124CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
title |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
spellingShingle |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs Navarro, Francisco Ezequiel Biología Celular, Microbiología Diatomeas Pared Celular Reconstrucción tridimensional |
title_short |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
title_full |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
title_fullStr |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
title_full_unstemmed |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
title_sort |
Three-Dimensional Reconstruction of Halamphora coffeaeformis Frustule from Scanning Electron Micrographs |
dc.creator.none.fl_str_mv |
Navarro, Francisco Ezequiel Popovich, Cecilia Sánchez, Miguel Costilla, Ignacio Gutierrez Ayesta, Cecilia Leonardi, Patricia |
author |
Navarro, Francisco Ezequiel |
author_facet |
Navarro, Francisco Ezequiel Popovich, Cecilia Sánchez, Miguel Costilla, Ignacio Gutierrez Ayesta, Cecilia Leonardi, Patricia |
author_role |
author |
author2 |
Popovich, Cecilia Sánchez, Miguel Costilla, Ignacio Gutierrez Ayesta, Cecilia Leonardi, Patricia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Biología Celular, Microbiología Diatomeas Pared Celular Reconstrucción tridimensional |
topic |
Biología Celular, Microbiología Diatomeas Pared Celular Reconstrucción tridimensional |
dc.description.none.fl_txt_mv |
The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications. Presentado en: 15th Interamerican Microscopy Congress CIASEM - SAMIC; October 1–4, 2019, Buenos Aires, Argentina |
description |
The diatom Halamphora coffeaeformis represents an alternative feedstock of oils for the biodiesel production. In addition, its siliceous cell wall (named frustule) presents possible industrial applications that have not been reported in the literature yet. Thus, the main objective of this study was to perform a three-dimensional reconstruction of H. coffeaeformis´ frustule from scanning electron micrographs, in order to have a study model. For this, the biomass of the species growing in a photobioreactor was harvested and treated as follows: a) some samples were dehydrated and subjected to a critical point; and b) other samples were treated with H2O2 in order to analyse the frustule´s ornamental pattern. All samples were metallized with gold, using an Argon plasma metal evaporator (Pelco model3) and they were observed with a LEO Scanning Electron Microscope, model EVO 40 XVP 2004. The frustules were elliptical to lanceolate with protracted ends. Valves presented convex dorsal margin and straight ventral margin. Dorsal striae were biseriate and radiate; ventral striae were short, uniseriate, radiate at the center and convergent at the ends of the valves. The raphe was straight and close to the ventral margin. The micrographs were analysed and separated into layers with the Adobe Photoshop CS6 software. The layers were vectorised with the Inkscape software and loaded and assembled in the Tinkercad software. The final aspects of the digital model were moulded on the page https://stephaneginier.com/sculptgl/. Finally, 3D printing was done with a CoLiDoTM 3.0 L printer, obtaining a three-dimensional replica representative of the H. coffeaeformis´ morphology . This replica will serve as a model for studies of its frustules related to nanotechnology and /or catalyst support applications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-28 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/11334 |
url |
https://digital.cic.gba.gob.ar/handle/11746/11334 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927620001154 info:eu-repo/semantics/altIdentifier/issn/1431-9276 info:eu-repo/semantics/altIdentifier/issn/1435-8115 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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