The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system

Autores
Vetter, D.E.; Katz, E.; Maison, S.F.; Taranda, J.N.; Turcan, S.; Ballestero, J.; Liberman, M.C.; Elgoyhen, A.B.N.; Boulter, J.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.
Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Proc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599
Materia
Cochlea
Electrophysiology
Inner hair cells
Outer hair cells
nicotinic receptor
nicotinic receptor alpha10
receptor subunit
unclassified drug
Chrna10 protein, mouse
Chrna9 protein, mouse
nicotinic receptor
animal cell
animal experiment
article
cell function
cell structure
cochlea
controlled study
electrophysiology
hair cell
innervation
mouse
neurophysiology
nonhuman
null allele
phenotype
priority journal
synapse
animal
cytology
electrostimulation
genetics
hair cell
mouse mutant
olivary nucleus
physiology
Mus
Animals
Cochlea
Electric Stimulation
Electrophysiology
Hair Cells, Auditory
Mice
Mice, Knockout
Olivary Nucleus
Receptors, Nicotinic
Synapses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00278424_v104_n51_p20594_Vetter

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oai_identifier_str paperaa:paper_00278424_v104_n51_p20594_Vetter
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear systemVetter, D.E.Katz, E.Maison, S.F.Taranda, J.N.Turcan, S.Ballestero, J.Liberman, M.C.Elgoyhen, A.B.N.Boulter, J.CochleaElectrophysiologyInner hair cellsOuter hair cellsnicotinic receptornicotinic receptor alpha10receptor subunitunclassified drugChrna10 protein, mouseChrna9 protein, mousenicotinic receptoranimal cellanimal experimentarticlecell functioncell structurecochleacontrolled studyelectrophysiologyhair cellinnervationmouseneurophysiologynonhumannull allelephenotypepriority journalsynapseanimalcytologyelectrostimulationgeneticshair cellmouse mutantolivary nucleusphysiologyMusAnimalsCochleaElectric StimulationElectrophysiologyHair Cells, AuditoryMiceMice, KnockoutOlivary NucleusReceptors, NicotinicSynapsesAlthough homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_VetterProc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:03Zpaperaa:paper_00278424_v104_n51_p20594_VetterInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:05.009Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
title The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
spellingShingle The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
Vetter, D.E.
Cochlea
Electrophysiology
Inner hair cells
Outer hair cells
nicotinic receptor
nicotinic receptor alpha10
receptor subunit
unclassified drug
Chrna10 protein, mouse
Chrna9 protein, mouse
nicotinic receptor
animal cell
animal experiment
article
cell function
cell structure
cochlea
controlled study
electrophysiology
hair cell
innervation
mouse
neurophysiology
nonhuman
null allele
phenotype
priority journal
synapse
animal
cytology
electrostimulation
genetics
hair cell
mouse mutant
olivary nucleus
physiology
Mus
Animals
Cochlea
Electric Stimulation
Electrophysiology
Hair Cells, Auditory
Mice
Mice, Knockout
Olivary Nucleus
Receptors, Nicotinic
Synapses
title_short The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
title_full The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
title_fullStr The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
title_full_unstemmed The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
title_sort The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
dc.creator.none.fl_str_mv Vetter, D.E.
Katz, E.
Maison, S.F.
Taranda, J.N.
Turcan, S.
Ballestero, J.
Liberman, M.C.
Elgoyhen, A.B.N.
Boulter, J.
author Vetter, D.E.
author_facet Vetter, D.E.
Katz, E.
Maison, S.F.
Taranda, J.N.
Turcan, S.
Ballestero, J.
Liberman, M.C.
Elgoyhen, A.B.N.
Boulter, J.
author_role author
author2 Katz, E.
Maison, S.F.
Taranda, J.N.
Turcan, S.
Ballestero, J.
Liberman, M.C.
Elgoyhen, A.B.N.
Boulter, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cochlea
Electrophysiology
Inner hair cells
Outer hair cells
nicotinic receptor
nicotinic receptor alpha10
receptor subunit
unclassified drug
Chrna10 protein, mouse
Chrna9 protein, mouse
nicotinic receptor
animal cell
animal experiment
article
cell function
cell structure
cochlea
controlled study
electrophysiology
hair cell
innervation
mouse
neurophysiology
nonhuman
null allele
phenotype
priority journal
synapse
animal
cytology
electrostimulation
genetics
hair cell
mouse mutant
olivary nucleus
physiology
Mus
Animals
Cochlea
Electric Stimulation
Electrophysiology
Hair Cells, Auditory
Mice
Mice, Knockout
Olivary Nucleus
Receptors, Nicotinic
Synapses
topic Cochlea
Electrophysiology
Inner hair cells
Outer hair cells
nicotinic receptor
nicotinic receptor alpha10
receptor subunit
unclassified drug
Chrna10 protein, mouse
Chrna9 protein, mouse
nicotinic receptor
animal cell
animal experiment
article
cell function
cell structure
cochlea
controlled study
electrophysiology
hair cell
innervation
mouse
neurophysiology
nonhuman
null allele
phenotype
priority journal
synapse
animal
cytology
electrostimulation
genetics
hair cell
mouse mutant
olivary nucleus
physiology
Mus
Animals
Cochlea
Electric Stimulation
Electrophysiology
Hair Cells, Auditory
Mice
Mice, Knockout
Olivary Nucleus
Receptors, Nicotinic
Synapses
dc.description.none.fl_txt_mv Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.
Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_Vetter
url http://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_Vetter
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Proc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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