The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
- Autores
- Vetter, D.E.; Katz, E.; Maison, S.F.; Taranda, J.N.; Turcan, S.; Ballestero, J.; Liberman, M.C.; Elgoyhen, A.B.N.; Boulter, J.
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.
Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Proc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599
- Materia
-
Cochlea
Electrophysiology
Inner hair cells
Outer hair cells
nicotinic receptor
nicotinic receptor alpha10
receptor subunit
unclassified drug
Chrna10 protein, mouse
Chrna9 protein, mouse
nicotinic receptor
animal cell
animal experiment
article
cell function
cell structure
cochlea
controlled study
electrophysiology
hair cell
innervation
mouse
neurophysiology
nonhuman
null allele
phenotype
priority journal
synapse
animal
cytology
electrostimulation
genetics
hair cell
mouse mutant
olivary nucleus
physiology
Mus
Animals
Cochlea
Electric Stimulation
Electrophysiology
Hair Cells, Auditory
Mice
Mice, Knockout
Olivary Nucleus
Receptors, Nicotinic
Synapses - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00278424_v104_n51_p20594_Vetter
Ver los metadatos del registro completo
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The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear systemVetter, D.E.Katz, E.Maison, S.F.Taranda, J.N.Turcan, S.Ballestero, J.Liberman, M.C.Elgoyhen, A.B.N.Boulter, J.CochleaElectrophysiologyInner hair cellsOuter hair cellsnicotinic receptornicotinic receptor alpha10receptor subunitunclassified drugChrna10 protein, mouseChrna9 protein, mousenicotinic receptoranimal cellanimal experimentarticlecell functioncell structurecochleacontrolled studyelectrophysiologyhair cellinnervationmouseneurophysiologynonhumannull allelephenotypepriority journalsynapseanimalcytologyelectrostimulationgeneticshair cellmouse mutantolivary nucleusphysiologyMusAnimalsCochleaElectric StimulationElectrophysiologyHair Cells, AuditoryMiceMice, KnockoutOlivary NucleusReceptors, NicotinicSynapsesAlthough homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA.Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_VetterProc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:03Zpaperaa:paper_00278424_v104_n51_p20594_VetterInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:05.009Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
title |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
spellingShingle |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system Vetter, D.E. Cochlea Electrophysiology Inner hair cells Outer hair cells nicotinic receptor nicotinic receptor alpha10 receptor subunit unclassified drug Chrna10 protein, mouse Chrna9 protein, mouse nicotinic receptor animal cell animal experiment article cell function cell structure cochlea controlled study electrophysiology hair cell innervation mouse neurophysiology nonhuman null allele phenotype priority journal synapse animal cytology electrostimulation genetics hair cell mouse mutant olivary nucleus physiology Mus Animals Cochlea Electric Stimulation Electrophysiology Hair Cells, Auditory Mice Mice, Knockout Olivary Nucleus Receptors, Nicotinic Synapses |
title_short |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
title_full |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
title_fullStr |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
title_full_unstemmed |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
title_sort |
The α10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system |
dc.creator.none.fl_str_mv |
Vetter, D.E. Katz, E. Maison, S.F. Taranda, J.N. Turcan, S. Ballestero, J. Liberman, M.C. Elgoyhen, A.B.N. Boulter, J. |
author |
Vetter, D.E. |
author_facet |
Vetter, D.E. Katz, E. Maison, S.F. Taranda, J.N. Turcan, S. Ballestero, J. Liberman, M.C. Elgoyhen, A.B.N. Boulter, J. |
author_role |
author |
author2 |
Katz, E. Maison, S.F. Taranda, J.N. Turcan, S. Ballestero, J. Liberman, M.C. Elgoyhen, A.B.N. Boulter, J. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Cochlea Electrophysiology Inner hair cells Outer hair cells nicotinic receptor nicotinic receptor alpha10 receptor subunit unclassified drug Chrna10 protein, mouse Chrna9 protein, mouse nicotinic receptor animal cell animal experiment article cell function cell structure cochlea controlled study electrophysiology hair cell innervation mouse neurophysiology nonhuman null allele phenotype priority journal synapse animal cytology electrostimulation genetics hair cell mouse mutant olivary nucleus physiology Mus Animals Cochlea Electric Stimulation Electrophysiology Hair Cells, Auditory Mice Mice, Knockout Olivary Nucleus Receptors, Nicotinic Synapses |
topic |
Cochlea Electrophysiology Inner hair cells Outer hair cells nicotinic receptor nicotinic receptor alpha10 receptor subunit unclassified drug Chrna10 protein, mouse Chrna9 protein, mouse nicotinic receptor animal cell animal experiment article cell function cell structure cochlea controlled study electrophysiology hair cell innervation mouse neurophysiology nonhuman null allele phenotype priority journal synapse animal cytology electrostimulation genetics hair cell mouse mutant olivary nucleus physiology Mus Animals Cochlea Electric Stimulation Electrophysiology Hair Cells, Auditory Mice Mice, Knockout Olivary Nucleus Receptors, Nicotinic Synapses |
dc.description.none.fl_txt_mv |
Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA. Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taranda, J.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ballestero, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
Although homomeric channels assembled from the α9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both α9 and α10 subunits. To gain insight into α10 subunit function in vivo, we examined olivocochlear innervation and function in α10 null-mutant mice. Electrophysiological recordings from postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents in α10 +/+ and α10+/- mice, with no detectable responses to ACh in α10+/+ mice. In contrast, a proportion of α10-/- outer hair cells showed small ACh-evoked currents. In α10-/- mutant mice, olivocochlear fiber stimulation failed to suppress distortion products, suggesting that the residual α9 homomeric nAChRs expressed by outer hair cells are unable to transduce efferent signals in vivo. Finally, α10-/- mice exhibit both an abnormal olivocochlear morphology and innervation to outer hair cells and a highly disorganized efferent innervation to the inner hair cell region. Our results demonstrate that α9-/- and α10-/- mice have overlapping but nonidentical phenotypes. Moreover, α10 nAChR subunits are required for normal olivocochlear activity because α9 homomeric nAChRs do not support maintenance of normal olivocochlear innervation or function in α10-/- mutant mice. © 2007 by The National Academy of Sciences of the USA. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_Vetter |
url |
http://hdl.handle.net/20.500.12110/paper_00278424_v104_n51_p20594_Vetter |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Proc. Natl. Acad. Sci. U. S. A. 2007;104(51):20594-20599 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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1846142844300951552 |
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12.712165 |