Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density

Autores
Arias, L.N.; Sambucetti, P.; Scannapieco, A.C.; Loeschcke, V.; Norry, F.M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd.
Fil:Arias, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Sambucetti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Scannapieco, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Exp. Biol. 2012;215(13):2220-2225
Materia
High temperature stress
Quantitative trait loci
Thermal resistance
Thermotolerance
adaptation
animal
article
Drosophila melanogaster
genetics
heat
larva
phenotype
physiological stress
physiology
quantitative trait locus
Adaptation, Physiological
Animals
Drosophila melanogaster
Hot Temperature
Larva
Phenotype
Quantitative Trait Loci
Stress, Physiological
Drosophila melanogaster
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00220949_v215_n13_p2220_Arias
Acceder
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oai_identifier_str paperaa:paper_00220949_v215_n13_p2220_Arias
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval densityArias, L.N.Sambucetti, P.Scannapieco, A.C.Loeschcke, V.Norry, F.M.High temperature stressQuantitative trait lociThermal resistanceThermotoleranceadaptationanimalarticleDrosophila melanogastergeneticsheatlarvaphenotypephysiological stressphysiologyquantitative trait locusAdaptation, PhysiologicalAnimalsDrosophila melanogasterHot TemperatureLarvaPhenotypeQuantitative Trait LociStress, PhysiologicalDrosophila melanogasterSurvival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd.Fil:Arias, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Sambucetti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Scannapieco, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_AriasJ. Exp. Biol. 2012;215(13):2220-2225reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-23T11:18:19Zpaperaa:paper_00220949_v215_n13_p2220_AriasInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-23 11:18:20.183Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
title Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
spellingShingle Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
Arias, L.N.
High temperature stress
Quantitative trait loci
Thermal resistance
Thermotolerance
adaptation
animal
article
Drosophila melanogaster
genetics
heat
larva
phenotype
physiological stress
physiology
quantitative trait locus
Adaptation, Physiological
Animals
Drosophila melanogaster
Hot Temperature
Larva
Phenotype
Quantitative Trait Loci
Stress, Physiological
Drosophila melanogaster
title_short Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
title_full Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
title_fullStr Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
title_full_unstemmed Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
title_sort Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
dc.creator.none.fl_str_mv Arias, L.N.
Sambucetti, P.
Scannapieco, A.C.
Loeschcke, V.
Norry, F.M.
author Arias, L.N.
author_facet Arias, L.N.
Sambucetti, P.
Scannapieco, A.C.
Loeschcke, V.
Norry, F.M.
author_role author
author2 Sambucetti, P.
Scannapieco, A.C.
Loeschcke, V.
Norry, F.M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv High temperature stress
Quantitative trait loci
Thermal resistance
Thermotolerance
adaptation
animal
article
Drosophila melanogaster
genetics
heat
larva
phenotype
physiological stress
physiology
quantitative trait locus
Adaptation, Physiological
Animals
Drosophila melanogaster
Hot Temperature
Larva
Phenotype
Quantitative Trait Loci
Stress, Physiological
Drosophila melanogaster
topic High temperature stress
Quantitative trait loci
Thermal resistance
Thermotolerance
adaptation
animal
article
Drosophila melanogaster
genetics
heat
larva
phenotype
physiological stress
physiology
quantitative trait locus
Adaptation, Physiological
Animals
Drosophila melanogaster
Hot Temperature
Larva
Phenotype
Quantitative Trait Loci
Stress, Physiological
Drosophila melanogaster
dc.description.none.fl_txt_mv Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd.
Fil:Arias, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Sambucetti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Scannapieco, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_Arias
url http://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_Arias
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Exp. Biol. 2012;215(13):2220-2225
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.982451

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