Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density

Autores
Arias, Leticia Noemi; Sambucetti, Pablo Daniel; Scannapieco, Alejandra Carla; Loeschcke, Volker; Norry, Fabian Marcelo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila  melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and  RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening  treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E–16F6; 30A3–34C2; 49C–50C). Previously found thermotolerance QTL were also significant for flies from HD cultures.
Fil: Arias, Leticia Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sambucetti, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loeschcke, Volker. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Norry, Fabian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
HIGH TEMPERATURE STRESS
QUANTITATIVE TRAIT LOCI
THERMAL RESISTANCE
THERMOTOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195176
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195176
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval densityArias, Leticia NoemiSambucetti, Pablo DanielScannapieco, Alejandra CarlaLoeschcke, VolkerNorry, Fabian MarceloHIGH TEMPERATURE STRESSQUANTITATIVE TRAIT LOCITHERMAL RESISTANCETHERMOTOLERANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila  melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and  RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening  treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E–16F6; 30A3–34C2; 49C–50C). Previously found thermotolerance QTL were also significant for flies from HD cultures.Fil: Arias, Leticia Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sambucetti, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scannapieco, Alejandra Carla. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Loeschcke, Volker. Aarhus University. Aarhus Institute Of Advanced Studies.; DinamarcaFil: Norry, Fabian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaCompany of Biologists2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195176Arias, Leticia Noemi; Sambucetti, Pablo Daniel; Scannapieco, Alejandra Carla; Loeschcke, Volker; Norry, Fabian Marcelo; Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density; Company of Biologists; Journal of Experimental Biology; 215; 13; 3-2012; 2220-22250022-0949CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jeb/article/215/13/2220/10885/Survival-of-heat-stress-with-and-without-heatinfo:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.069831info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:39Zoai:ri.conicet.gov.ar:11336/195176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:40.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
title Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
spellingShingle Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
Arias, Leticia Noemi
HIGH TEMPERATURE STRESS
QUANTITATIVE TRAIT LOCI
THERMAL RESISTANCE
THERMOTOLERANCE
title_short Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
title_full Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
title_fullStr Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
title_full_unstemmed Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
title_sort Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density
dc.creator.none.fl_str_mv Arias, Leticia Noemi
Sambucetti, Pablo Daniel
Scannapieco, Alejandra Carla
Loeschcke, Volker
Norry, Fabian Marcelo
author Arias, Leticia Noemi
author_facet Arias, Leticia Noemi
Sambucetti, Pablo Daniel
Scannapieco, Alejandra Carla
Loeschcke, Volker
Norry, Fabian Marcelo
author_role author
author2 Sambucetti, Pablo Daniel
Scannapieco, Alejandra Carla
Loeschcke, Volker
Norry, Fabian Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv HIGH TEMPERATURE STRESS
QUANTITATIVE TRAIT LOCI
THERMAL RESISTANCE
THERMOTOLERANCE
topic HIGH TEMPERATURE STRESS
QUANTITATIVE TRAIT LOCI
THERMAL RESISTANCE
THERMOTOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila  melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and  RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening  treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E–16F6; 30A3–34C2; 49C–50C). Previously found thermotolerance QTL were also significant for flies from HD cultures.
Fil: Arias, Leticia Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sambucetti, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Loeschcke, Volker. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Norry, Fabian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila  melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and  RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening  treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E–16F6; 30A3–34C2; 49C–50C). Previously found thermotolerance QTL were also significant for flies from HD cultures.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195176
Arias, Leticia Noemi; Sambucetti, Pablo Daniel; Scannapieco, Alejandra Carla; Loeschcke, Volker; Norry, Fabian Marcelo; Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density; Company of Biologists; Journal of Experimental Biology; 215; 13; 3-2012; 2220-2225
0022-0949
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195176
identifier_str_mv Arias, Leticia Noemi; Sambucetti, Pablo Daniel; Scannapieco, Alejandra Carla; Loeschcke, Volker; Norry, Fabian Marcelo; Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density; Company of Biologists; Journal of Experimental Biology; 215; 13; 3-2012; 2220-2225
0022-0949
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jeb/article/215/13/2220/10885/Survival-of-heat-stress-with-and-without-heat
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.069831
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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