Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression

• Autores: González, I.T.; Barrientos, G.; Freitag, N.; Otto, T.; Thijssen, V.L.J.L.; Moschansky, P.; von Kwiatkowski, P.; Klapp, B.F.; Winterhager, E.; Bauersachs, S.; Blois, S.M.
• Año de publicación: 2012
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: PLoS ONE 2012;7(10)
• Materia: angiopoietin 1; glycoprotein p 15095; animal cell; animal tissue; article; cell differentiation; cell expansion; cell interaction; cell proliferation; cell shape; controlled study; decidualization; dendritic cell; female; first trimester pregnancy; flow cytometry; fluorescence activated cell sorting; gene expression; histopathology; immunogenicity; immunohistochemistry; inflammation; mouse; natural killer cell; nidation; nonhuman; pregnancy; pregnancy disorder; quantitative analysis; reverse transcription polymerase chain reaction; stroma cell; T cell depletion; uterine cervix; Animals; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Female; Immunohistochemistry; Killer Cells, Natural; Male; Mice; Oligonucleotide Array Sequence Analysis; Pregnancy; Reverse Transcriptase Polymerase Chain Reaction; Uterus
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al.