Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes

Autores
Peressin, Maryse; Proust, Alize; Schmidt, Sylvie; Su, Bin; Lambotin, Melanie; Biedma, Marina Elizabeth; Laumond, Geraldine; Decoville, Thomas; Holl, Vincent; Moog, Christiane
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The chronology of HIV infection in mucosal tissue after sexual transmission is unknown. Several potential HIV target cells are present at these sites, including dendritic cells, macrophages, and CD4þ T lymphocytes. Dendritic cells and macrophages are antigen-presenting cells (APCs) and are thus involved in cross-talk with T cells. This close contact may favor efficient HIV-1 transfer to T lymphocytes, resulting in rapid HIV-1 dissemination. Design: We investigated the role of APCs in HIV transfer to T cells by incubating Langerhans cells and interstitial dendritic cells (IDCs) or monocyte-derived macrophages (MDMs) with HIV for 2 h before addition of uninfected autologous CD4þ T lymphocytes. Methods: HIV infection was recorded after different time points. Following staining, the measurement of intracellular p24 in the different cell populations was analyzed by flow cytometry. Results: We showed that Langerhans cells/IDCs and macrophages efficiently transferred HIV to CD4þ T cells. Interestingly, a rapid HIV transfer in trans predominated in MDMs, whereas cis transfer mainly occurred in Langerhans cells/IDC cocultures. Neutralizing antibody 2G12, added to HIV-loaded APCs, efficiently blocked both the trans and the cis infection of T cells. Conclusion: These findings highlight the major contributions of various mucosal cells in HIV dissemination and suggest that HIV hijacks the different properties of APCs to favor its dissemination through the body. They emphasize the role of macrophages in the rapid transmission of HIV to T lymphocytes at mucosal sites, dendritic cells being prone to migration to lymphoid organ for subsequent dissemination by cis transfer.
Fil: Peressin, Maryse. Université de Strasbourg; Francia
Fil: Proust, Alize. Université de Strasbourg; Francia
Fil: Schmidt, Sylvie. Université de Strasbourg; Francia
Fil: Su, Bin. Université de Strasbourg; Francia
Fil: Lambotin, Melanie. Université de Strasbourg; Francia
Fil: Biedma, Marina Elizabeth. Université de Strasbourg; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laumond, Geraldine. Université de Strasbourg; Francia
Fil: Decoville, Thomas. Université de Strasbourg; Francia
Fil: Holl, Vincent. Université de Strasbourg; Francia
Fil: Moog, Christiane. Université de Strasbourg; Francia
Materia
Hiv
Cell-To-Cell Transfer
Langerhans Dendritic Cells
Macrophages
Lymphocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24706
Acceder
id CONICETDig_b168dd2a596bad6dacfe0728cac3b19c
oai_identifier_str oai:ri.conicet.gov.ar:11336/24706
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytesPeressin, MaryseProust, AlizeSchmidt, SylvieSu, BinLambotin, MelanieBiedma, Marina ElizabethLaumond, GeraldineDecoville, ThomasHoll, VincentMoog, ChristianeHivCell-To-Cell TransferLangerhans Dendritic CellsMacrophagesLymphocyteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: The chronology of HIV infection in mucosal tissue after sexual transmission is unknown. Several potential HIV target cells are present at these sites, including dendritic cells, macrophages, and CD4þ T lymphocytes. Dendritic cells and macrophages are antigen-presenting cells (APCs) and are thus involved in cross-talk with T cells. This close contact may favor efficient HIV-1 transfer to T lymphocytes, resulting in rapid HIV-1 dissemination. Design: We investigated the role of APCs in HIV transfer to T cells by incubating Langerhans cells and interstitial dendritic cells (IDCs) or monocyte-derived macrophages (MDMs) with HIV for 2 h before addition of uninfected autologous CD4þ T lymphocytes. Methods: HIV infection was recorded after different time points. Following staining, the measurement of intracellular p24 in the different cell populations was analyzed by flow cytometry. Results: We showed that Langerhans cells/IDCs and macrophages efficiently transferred HIV to CD4þ T cells. Interestingly, a rapid HIV transfer in trans predominated in MDMs, whereas cis transfer mainly occurred in Langerhans cells/IDC cocultures. Neutralizing antibody 2G12, added to HIV-loaded APCs, efficiently blocked both the trans and the cis infection of T cells. Conclusion: These findings highlight the major contributions of various mucosal cells in HIV dissemination and suggest that HIV hijacks the different properties of APCs to favor its dissemination through the body. They emphasize the role of macrophages in the rapid transmission of HIV to T lymphocytes at mucosal sites, dendritic cells being prone to migration to lymphoid organ for subsequent dissemination by cis transfer.Fil: Peressin, Maryse. Université de Strasbourg; FranciaFil: Proust, Alize. Université de Strasbourg; FranciaFil: Schmidt, Sylvie. Université de Strasbourg; FranciaFil: Su, Bin. Université de Strasbourg; FranciaFil: Lambotin, Melanie. Université de Strasbourg; FranciaFil: Biedma, Marina Elizabeth. Université de Strasbourg; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laumond, Geraldine. Université de Strasbourg; FranciaFil: Decoville, Thomas. Université de Strasbourg; FranciaFil: Holl, Vincent. Université de Strasbourg; FranciaFil: Moog, Christiane. Université de Strasbourg; FranciaLippincott Williams2014-03-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24706Peressin, Maryse; Proust, Alize; Schmidt, Sylvie; Su, Bin; Lambotin, Melanie; et al.; Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes; Lippincott Williams; Aids; 28; 5; 13-3-2014; 667-6770269-9370CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1097/QAD.0000000000000193info:eu-repo/semantics/altIdentifier/url/http://ovidsp.tx.ovid.com/sp-3.26.1a/ovidweb.cgi?QS2=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:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/aidsonline/pages/articleviewer.aspx?year=2014&issue=03130&article=00004&type=abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:23:18Zoai:ri.conicet.gov.ar:11336/24706instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:23:18.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
title Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
spellingShingle Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
Peressin, Maryse
Hiv
Cell-To-Cell Transfer
Langerhans Dendritic Cells
Macrophages
Lymphocytes
title_short Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
title_full Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
title_fullStr Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
title_full_unstemmed Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
title_sort Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes
dc.creator.none.fl_str_mv Peressin, Maryse
Proust, Alize
Schmidt, Sylvie
Su, Bin
Lambotin, Melanie
Biedma, Marina Elizabeth
Laumond, Geraldine
Decoville, Thomas
Holl, Vincent
Moog, Christiane
author Peressin, Maryse
author_facet Peressin, Maryse
Proust, Alize
Schmidt, Sylvie
Su, Bin
Lambotin, Melanie
Biedma, Marina Elizabeth
Laumond, Geraldine
Decoville, Thomas
Holl, Vincent
Moog, Christiane
author_role author
author2 Proust, Alize
Schmidt, Sylvie
Su, Bin
Lambotin, Melanie
Biedma, Marina Elizabeth
Laumond, Geraldine
Decoville, Thomas
Holl, Vincent
Moog, Christiane
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hiv
Cell-To-Cell Transfer
Langerhans Dendritic Cells
Macrophages
Lymphocytes
topic Hiv
Cell-To-Cell Transfer
Langerhans Dendritic Cells
Macrophages
Lymphocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: The chronology of HIV infection in mucosal tissue after sexual transmission is unknown. Several potential HIV target cells are present at these sites, including dendritic cells, macrophages, and CD4þ T lymphocytes. Dendritic cells and macrophages are antigen-presenting cells (APCs) and are thus involved in cross-talk with T cells. This close contact may favor efficient HIV-1 transfer to T lymphocytes, resulting in rapid HIV-1 dissemination. Design: We investigated the role of APCs in HIV transfer to T cells by incubating Langerhans cells and interstitial dendritic cells (IDCs) or monocyte-derived macrophages (MDMs) with HIV for 2 h before addition of uninfected autologous CD4þ T lymphocytes. Methods: HIV infection was recorded after different time points. Following staining, the measurement of intracellular p24 in the different cell populations was analyzed by flow cytometry. Results: We showed that Langerhans cells/IDCs and macrophages efficiently transferred HIV to CD4þ T cells. Interestingly, a rapid HIV transfer in trans predominated in MDMs, whereas cis transfer mainly occurred in Langerhans cells/IDC cocultures. Neutralizing antibody 2G12, added to HIV-loaded APCs, efficiently blocked both the trans and the cis infection of T cells. Conclusion: These findings highlight the major contributions of various mucosal cells in HIV dissemination and suggest that HIV hijacks the different properties of APCs to favor its dissemination through the body. They emphasize the role of macrophages in the rapid transmission of HIV to T lymphocytes at mucosal sites, dendritic cells being prone to migration to lymphoid organ for subsequent dissemination by cis transfer.
Fil: Peressin, Maryse. Université de Strasbourg; Francia
Fil: Proust, Alize. Université de Strasbourg; Francia
Fil: Schmidt, Sylvie. Université de Strasbourg; Francia
Fil: Su, Bin. Université de Strasbourg; Francia
Fil: Lambotin, Melanie. Université de Strasbourg; Francia
Fil: Biedma, Marina Elizabeth. Université de Strasbourg; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laumond, Geraldine. Université de Strasbourg; Francia
Fil: Decoville, Thomas. Université de Strasbourg; Francia
Fil: Holl, Vincent. Université de Strasbourg; Francia
Fil: Moog, Christiane. Université de Strasbourg; Francia
description Objective: The chronology of HIV infection in mucosal tissue after sexual transmission is unknown. Several potential HIV target cells are present at these sites, including dendritic cells, macrophages, and CD4þ T lymphocytes. Dendritic cells and macrophages are antigen-presenting cells (APCs) and are thus involved in cross-talk with T cells. This close contact may favor efficient HIV-1 transfer to T lymphocytes, resulting in rapid HIV-1 dissemination. Design: We investigated the role of APCs in HIV transfer to T cells by incubating Langerhans cells and interstitial dendritic cells (IDCs) or monocyte-derived macrophages (MDMs) with HIV for 2 h before addition of uninfected autologous CD4þ T lymphocytes. Methods: HIV infection was recorded after different time points. Following staining, the measurement of intracellular p24 in the different cell populations was analyzed by flow cytometry. Results: We showed that Langerhans cells/IDCs and macrophages efficiently transferred HIV to CD4þ T cells. Interestingly, a rapid HIV transfer in trans predominated in MDMs, whereas cis transfer mainly occurred in Langerhans cells/IDC cocultures. Neutralizing antibody 2G12, added to HIV-loaded APCs, efficiently blocked both the trans and the cis infection of T cells. Conclusion: These findings highlight the major contributions of various mucosal cells in HIV dissemination and suggest that HIV hijacks the different properties of APCs to favor its dissemination through the body. They emphasize the role of macrophages in the rapid transmission of HIV to T lymphocytes at mucosal sites, dendritic cells being prone to migration to lymphoid organ for subsequent dissemination by cis transfer.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24706
Peressin, Maryse; Proust, Alize; Schmidt, Sylvie; Su, Bin; Lambotin, Melanie; et al.; Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes; Lippincott Williams; Aids; 28; 5; 13-3-2014; 667-677
0269-9370
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24706
identifier_str_mv Peressin, Maryse; Proust, Alize; Schmidt, Sylvie; Su, Bin; Lambotin, Melanie; et al.; Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes; Lippincott Williams; Aids; 28; 5; 13-3-2014; 667-677
0269-9370
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1097/QAD.0000000000000193
info:eu-repo/semantics/altIdentifier/url/http://ovidsp.tx.ovid.com/sp-3.26.1a/ovidweb.cgi?QS2=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
info:eu-repo/semantics/altIdentifier/url/http://journals.lww.com/aidsonline/pages/articleviewer.aspx?year=2014&issue=03130&article=00004&type=abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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