Allatotropin: An ancestral myotropic neuropeptide involved in feeding

Autores
Alzugaray, Maria Eugenia; Adami, Mariana Laura; Diambra, Luis Anibal; Hernandez Martinez, Salvador; Damborenea, Maria Cristina; Noriega, Fernando Gabriel; Ronderos, Jorge Rafael
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Cell-cell interactions are a basic principle for the organization of tissues and organs allowing them to perform integrated functions and to organize themselves spatially and temporally. Peptidic molecules secreted by neurons and epithelial cells play fundamental roles in cell-cell interactions, acting as local neuromodulators, neurohormones, as well as endocrine and paracrine messengers. Allatotropin (AT) is a neuropeptide originally described as a regulator of Juvenile Hormone synthesis, which plays multiple neural, endocrine and myoactive roles in insects and other organisms. Methods: A combination of immunohistochemistry using AT-antibodies and AT-Qdot nanocrystal conjugates was used to identify immunoreactive nerve cells containing the peptide and epithelial-muscular cells targeted by AT in Hydra plagiodesmica. Physiological assays using AT and AT- antibodies revealed that while AT stimulated the extrusion of the hypostome in a dose-response fashion in starved hydroids, the activity of hypostome in hydroids challenged with food was blocked by treatments with different doses of AT-antibodies. Conclusions: AT antibodies immunolabeled nerve cells in the stalk, pedal disc, tentacles and hypostome. AT-Qdot conjugates recognized epithelial-muscular cell in the same tissues, suggesting the existence of anatomical and functional relationships between these two cell populations. Physiological assays indicated that the AT-like peptide is facilitating food ingestion. Significance: Immunochemical, physiological and bioinformatics evidence advocates that AT is an ancestral neuropeptide involved in myoregulatory activities associated with meal ingestion and digestion.
Fil: Alzugaray, Maria Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
Fil: Adami, Mariana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Pública; México
Fil: Damborenea, Maria Cristina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Noriega, Fernando Gabriel. Florida International University; Estados Unidos
Fil: Ronderos, Jorge Rafael. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; Argentina
Materia
Invertebrates
Cell-Cell Interactions
Cnidaria
Immunohistochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2405
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allatotropin: An ancestral myotropic neuropeptide involved in feedingAlzugaray, Maria EugeniaAdami, Mariana LauraDiambra, Luis AnibalHernandez Martinez, SalvadorDamborenea, Maria CristinaNoriega, Fernando GabrielRonderos, Jorge RafaelInvertebratesCell-Cell InteractionsCnidariaImmunohistochemistryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Cell-cell interactions are a basic principle for the organization of tissues and organs allowing them to perform integrated functions and to organize themselves spatially and temporally. Peptidic molecules secreted by neurons and epithelial cells play fundamental roles in cell-cell interactions, acting as local neuromodulators, neurohormones, as well as endocrine and paracrine messengers. Allatotropin (AT) is a neuropeptide originally described as a regulator of Juvenile Hormone synthesis, which plays multiple neural, endocrine and myoactive roles in insects and other organisms. Methods: A combination of immunohistochemistry using AT-antibodies and AT-Qdot nanocrystal conjugates was used to identify immunoreactive nerve cells containing the peptide and epithelial-muscular cells targeted by AT in Hydra plagiodesmica. Physiological assays using AT and AT- antibodies revealed that while AT stimulated the extrusion of the hypostome in a dose-response fashion in starved hydroids, the activity of hypostome in hydroids challenged with food was blocked by treatments with different doses of AT-antibodies. Conclusions: AT antibodies immunolabeled nerve cells in the stalk, pedal disc, tentacles and hypostome. AT-Qdot conjugates recognized epithelial-muscular cell in the same tissues, suggesting the existence of anatomical and functional relationships between these two cell populations. Physiological assays indicated that the AT-like peptide is facilitating food ingestion. Significance: Immunochemical, physiological and bioinformatics evidence advocates that AT is an ancestral neuropeptide involved in myoregulatory activities associated with meal ingestion and digestion.Fil: Alzugaray, Maria Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; ArgentinaFil: Adami, Mariana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; ArgentinaFil: Diambra, Luis Anibal. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Pública; MéxicoFil: Damborenea, Maria Cristina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; ArgentinaFil: Noriega, Fernando Gabriel. Florida International University; Estados UnidosFil: Ronderos, Jorge Rafael. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; ArgentinaPublic Library of Science2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2405Alzugaray, Maria Eugenia; Adami, Mariana Laura; Diambra, Luis Anibal; Hernandez Martinez, Salvador; Damborenea, Maria Cristina; et al.; Allatotropin: An ancestral myotropic neuropeptide involved in feeding; Public Library of Science; Plos One; 8; 10; 10-2013; e77520-e775201932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0077520info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0077520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:17Zoai:ri.conicet.gov.ar:11336/2405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:17.653CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allatotropin: An ancestral myotropic neuropeptide involved in feeding
title Allatotropin: An ancestral myotropic neuropeptide involved in feeding
spellingShingle Allatotropin: An ancestral myotropic neuropeptide involved in feeding
Alzugaray, Maria Eugenia
Invertebrates
Cell-Cell Interactions
Cnidaria
Immunohistochemistry
title_short Allatotropin: An ancestral myotropic neuropeptide involved in feeding
title_full Allatotropin: An ancestral myotropic neuropeptide involved in feeding
title_fullStr Allatotropin: An ancestral myotropic neuropeptide involved in feeding
title_full_unstemmed Allatotropin: An ancestral myotropic neuropeptide involved in feeding
title_sort Allatotropin: An ancestral myotropic neuropeptide involved in feeding
dc.creator.none.fl_str_mv Alzugaray, Maria Eugenia
Adami, Mariana Laura
Diambra, Luis Anibal
Hernandez Martinez, Salvador
Damborenea, Maria Cristina
Noriega, Fernando Gabriel
Ronderos, Jorge Rafael
author Alzugaray, Maria Eugenia
author_facet Alzugaray, Maria Eugenia
Adami, Mariana Laura
Diambra, Luis Anibal
Hernandez Martinez, Salvador
Damborenea, Maria Cristina
Noriega, Fernando Gabriel
Ronderos, Jorge Rafael
author_role author
author2 Adami, Mariana Laura
Diambra, Luis Anibal
Hernandez Martinez, Salvador
Damborenea, Maria Cristina
Noriega, Fernando Gabriel
Ronderos, Jorge Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Invertebrates
Cell-Cell Interactions
Cnidaria
Immunohistochemistry
topic Invertebrates
Cell-Cell Interactions
Cnidaria
Immunohistochemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Cell-cell interactions are a basic principle for the organization of tissues and organs allowing them to perform integrated functions and to organize themselves spatially and temporally. Peptidic molecules secreted by neurons and epithelial cells play fundamental roles in cell-cell interactions, acting as local neuromodulators, neurohormones, as well as endocrine and paracrine messengers. Allatotropin (AT) is a neuropeptide originally described as a regulator of Juvenile Hormone synthesis, which plays multiple neural, endocrine and myoactive roles in insects and other organisms. Methods: A combination of immunohistochemistry using AT-antibodies and AT-Qdot nanocrystal conjugates was used to identify immunoreactive nerve cells containing the peptide and epithelial-muscular cells targeted by AT in Hydra plagiodesmica. Physiological assays using AT and AT- antibodies revealed that while AT stimulated the extrusion of the hypostome in a dose-response fashion in starved hydroids, the activity of hypostome in hydroids challenged with food was blocked by treatments with different doses of AT-antibodies. Conclusions: AT antibodies immunolabeled nerve cells in the stalk, pedal disc, tentacles and hypostome. AT-Qdot conjugates recognized epithelial-muscular cell in the same tissues, suggesting the existence of anatomical and functional relationships between these two cell populations. Physiological assays indicated that the AT-like peptide is facilitating food ingestion. Significance: Immunochemical, physiological and bioinformatics evidence advocates that AT is an ancestral neuropeptide involved in myoregulatory activities associated with meal ingestion and digestion.
Fil: Alzugaray, Maria Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
Fil: Adami, Mariana Laura. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Pública; México
Fil: Damborenea, Maria Cristina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Noriega, Fernando Gabriel. Florida International University; Estados Unidos
Fil: Ronderos, Jorge Rafael. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de la Plata. Centro Regional de Estudios Genómicos; Argentina
description Background: Cell-cell interactions are a basic principle for the organization of tissues and organs allowing them to perform integrated functions and to organize themselves spatially and temporally. Peptidic molecules secreted by neurons and epithelial cells play fundamental roles in cell-cell interactions, acting as local neuromodulators, neurohormones, as well as endocrine and paracrine messengers. Allatotropin (AT) is a neuropeptide originally described as a regulator of Juvenile Hormone synthesis, which plays multiple neural, endocrine and myoactive roles in insects and other organisms. Methods: A combination of immunohistochemistry using AT-antibodies and AT-Qdot nanocrystal conjugates was used to identify immunoreactive nerve cells containing the peptide and epithelial-muscular cells targeted by AT in Hydra plagiodesmica. Physiological assays using AT and AT- antibodies revealed that while AT stimulated the extrusion of the hypostome in a dose-response fashion in starved hydroids, the activity of hypostome in hydroids challenged with food was blocked by treatments with different doses of AT-antibodies. Conclusions: AT antibodies immunolabeled nerve cells in the stalk, pedal disc, tentacles and hypostome. AT-Qdot conjugates recognized epithelial-muscular cell in the same tissues, suggesting the existence of anatomical and functional relationships between these two cell populations. Physiological assays indicated that the AT-like peptide is facilitating food ingestion. Significance: Immunochemical, physiological and bioinformatics evidence advocates that AT is an ancestral neuropeptide involved in myoregulatory activities associated with meal ingestion and digestion.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2405
Alzugaray, Maria Eugenia; Adami, Mariana Laura; Diambra, Luis Anibal; Hernandez Martinez, Salvador; Damborenea, Maria Cristina; et al.; Allatotropin: An ancestral myotropic neuropeptide involved in feeding; Public Library of Science; Plos One; 8; 10; 10-2013; e77520-e77520
1932-6203
url http://hdl.handle.net/11336/2405
identifier_str_mv Alzugaray, Maria Eugenia; Adami, Mariana Laura; Diambra, Luis Anibal; Hernandez Martinez, Salvador; Damborenea, Maria Cristina; et al.; Allatotropin: An ancestral myotropic neuropeptide involved in feeding; Public Library of Science; Plos One; 8; 10; 10-2013; e77520-e77520
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0077520
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0077520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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