Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers

Autores
Fölling, Jonas; Belov, Vladimir; Riedel, Jennifer Denise; Schönle, Andreas; Egner, Alexander; Eggeling, Christian; Bossi, Mariano Luis; Hell, Stefan W.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During the last decade far-field fluorescence microscopy methodshave evolved that have resolution far below the wavelength oflight. To outperform the limiting role of diffraction, all thesemethods, in one way or another, switch the ability of a moleculeto emit fluorescence. Here we present a novel rhodamine amidethat can be photoswitched from a nonfluorescent to a fluorescentstate by absorption of one or two photons from a continuous-wave laser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precisiondown to 15 nm in the focal plane. Two-photon induced nonlinearphotoswitching of this marker with continuous-wave illuminationoffers optical sectioning with simple laser equipment.Future synthesis of similar compounds holds great promise forcost-effective fluorescence nanoscopy with noninvasive optical sectioning.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Riedel, Jennifer Denise. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
Materia
dyes/pigments
fluorescence microscopy
fluorescent probes
photoswitching
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102927
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave LasersFölling, JonasBelov, VladimirRiedel, Jennifer DeniseSchönle, AndreasEgner, AlexanderEggeling, ChristianBossi, Mariano LuisHell, Stefan W.dyes/pigmentsfluorescence microscopyfluorescent probesphotoswitchinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1During the last decade far-field fluorescence microscopy methodshave evolved that have resolution far below the wavelength oflight. To outperform the limiting role of diffraction, all thesemethods, in one way or another, switch the ability of a moleculeto emit fluorescence. Here we present a novel rhodamine amidethat can be photoswitched from a nonfluorescent to a fluorescentstate by absorption of one or two photons from a continuous-wave laser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precisiondown to 15 nm in the focal plane. Two-photon induced nonlinearphotoswitching of this marker with continuous-wave illuminationoffers optical sectioning with simple laser equipment.Future synthesis of similar compounds holds great promise forcost-effective fluorescence nanoscopy with noninvasive optical sectioning.Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Riedel, Jennifer Denise. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; AlemaniaWiley VCH Verlag2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102927Fölling, Jonas; Belov, Vladimir; Riedel, Jennifer Denise; Schönle, Andreas; Egner, Alexander; et al.; Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers; Wiley VCH Verlag; Chemphyschem; 9; 2; 2-2008; 321-3261439-4235CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cphc.200700655info:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.200700655info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:43Zoai:ri.conicet.gov.ar:11336/102927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:43.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
title Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
spellingShingle Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
Fölling, Jonas
dyes/pigments
fluorescence microscopy
fluorescent probes
photoswitching
title_short Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
title_full Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
title_fullStr Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
title_full_unstemmed Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
title_sort Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers
dc.creator.none.fl_str_mv Fölling, Jonas
Belov, Vladimir
Riedel, Jennifer Denise
Schönle, Andreas
Egner, Alexander
Eggeling, Christian
Bossi, Mariano Luis
Hell, Stefan W.
author Fölling, Jonas
author_facet Fölling, Jonas
Belov, Vladimir
Riedel, Jennifer Denise
Schönle, Andreas
Egner, Alexander
Eggeling, Christian
Bossi, Mariano Luis
Hell, Stefan W.
author_role author
author2 Belov, Vladimir
Riedel, Jennifer Denise
Schönle, Andreas
Egner, Alexander
Eggeling, Christian
Bossi, Mariano Luis
Hell, Stefan W.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv dyes/pigments
fluorescence microscopy
fluorescent probes
photoswitching
topic dyes/pigments
fluorescence microscopy
fluorescent probes
photoswitching
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During the last decade far-field fluorescence microscopy methodshave evolved that have resolution far below the wavelength oflight. To outperform the limiting role of diffraction, all thesemethods, in one way or another, switch the ability of a moleculeto emit fluorescence. Here we present a novel rhodamine amidethat can be photoswitched from a nonfluorescent to a fluorescentstate by absorption of one or two photons from a continuous-wave laser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precisiondown to 15 nm in the focal plane. Two-photon induced nonlinearphotoswitching of this marker with continuous-wave illuminationoffers optical sectioning with simple laser equipment.Future synthesis of similar compounds holds great promise forcost-effective fluorescence nanoscopy with noninvasive optical sectioning.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Riedel, Jennifer Denise. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
description During the last decade far-field fluorescence microscopy methodshave evolved that have resolution far below the wavelength oflight. To outperform the limiting role of diffraction, all thesemethods, in one way or another, switch the ability of a moleculeto emit fluorescence. Here we present a novel rhodamine amidethat can be photoswitched from a nonfluorescent to a fluorescentstate by absorption of one or two photons from a continuous-wave laser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precisiondown to 15 nm in the focal plane. Two-photon induced nonlinearphotoswitching of this marker with continuous-wave illuminationoffers optical sectioning with simple laser equipment.Future synthesis of similar compounds holds great promise forcost-effective fluorescence nanoscopy with noninvasive optical sectioning.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102927
Fölling, Jonas; Belov, Vladimir; Riedel, Jennifer Denise; Schönle, Andreas; Egner, Alexander; et al.; Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers; Wiley VCH Verlag; Chemphyschem; 9; 2; 2-2008; 321-326
1439-4235
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102927
identifier_str_mv Fölling, Jonas; Belov, Vladimir; Riedel, Jennifer Denise; Schönle, Andreas; Egner, Alexander; et al.; Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers; Wiley VCH Verlag; Chemphyschem; 9; 2; 2-2008; 321-326
1439-4235
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cphc.200700655
info:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.200700655
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268747226677248
score 13.13397

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