A slow RNA polymerase II affects alternative splicing in vivo

Autores
De La Mata, M.; Alonso, C.R.; Kadener, S.; Fededa, J.P.; Blaustein, M.; Pelisch, F.; Cramer, P.; Bentley, D.; Kornblihtt, A.R.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.
Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Alonso, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Mol. Cell 2003;12(2):525-532
Materia
elongation factor
fibronectin
protein subunit
RNA
RNA polymerase II
Adenovirus
article
cell culture
Drosophila
embryo cell
exon
gene
genetic transcription
genetic transfection
Hox gene
human
in vivo study
point mutation
protein expression
reverse transcription polymerase chain reaction
RNA splicing
RNA translation
Western blotting
Adenoviridae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_10972765_v12_n2_p525_DeLaMata
Acceder
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oai_identifier_str paperaa:paper_10972765_v12_n2_p525_DeLaMata
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling A slow RNA polymerase II affects alternative splicing in vivoDe La Mata, M.Alonso, C.R.Kadener, S.Fededa, J.P.Blaustein, M.Pelisch, F.Cramer, P.Bentley, D.Kornblihtt, A.R.elongation factorfibronectinprotein subunitRNARNA polymerase IIAdenovirusarticlecell cultureDrosophilaembryo cellexongenegenetic transcriptiongenetic transfectionHox genehumanin vivo studypoint mutationprotein expressionreverse transcription polymerase chain reactionRNA splicingRNA translationWestern blottingAdenoviridaeChanges in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Alonso, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMataMol. Cell 2003;12(2):525-532reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:16Zpaperaa:paper_10972765_v12_n2_p525_DeLaMataInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:17.996Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv A slow RNA polymerase II affects alternative splicing in vivo
title A slow RNA polymerase II affects alternative splicing in vivo
spellingShingle A slow RNA polymerase II affects alternative splicing in vivo
De La Mata, M.
elongation factor
fibronectin
protein subunit
RNA
RNA polymerase II
Adenovirus
article
cell culture
Drosophila
embryo cell
exon
gene
genetic transcription
genetic transfection
Hox gene
human
in vivo study
point mutation
protein expression
reverse transcription polymerase chain reaction
RNA splicing
RNA translation
Western blotting
Adenoviridae
title_short A slow RNA polymerase II affects alternative splicing in vivo
title_full A slow RNA polymerase II affects alternative splicing in vivo
title_fullStr A slow RNA polymerase II affects alternative splicing in vivo
title_full_unstemmed A slow RNA polymerase II affects alternative splicing in vivo
title_sort A slow RNA polymerase II affects alternative splicing in vivo
dc.creator.none.fl_str_mv De La Mata, M.
Alonso, C.R.
Kadener, S.
Fededa, J.P.
Blaustein, M.
Pelisch, F.
Cramer, P.
Bentley, D.
Kornblihtt, A.R.
author De La Mata, M.
author_facet De La Mata, M.
Alonso, C.R.
Kadener, S.
Fededa, J.P.
Blaustein, M.
Pelisch, F.
Cramer, P.
Bentley, D.
Kornblihtt, A.R.
author_role author
author2 Alonso, C.R.
Kadener, S.
Fededa, J.P.
Blaustein, M.
Pelisch, F.
Cramer, P.
Bentley, D.
Kornblihtt, A.R.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv elongation factor
fibronectin
protein subunit
RNA
RNA polymerase II
Adenovirus
article
cell culture
Drosophila
embryo cell
exon
gene
genetic transcription
genetic transfection
Hox gene
human
in vivo study
point mutation
protein expression
reverse transcription polymerase chain reaction
RNA splicing
RNA translation
Western blotting
Adenoviridae
topic elongation factor
fibronectin
protein subunit
RNA
RNA polymerase II
Adenovirus
article
cell culture
Drosophila
embryo cell
exon
gene
genetic transcription
genetic transfection
Hox gene
human
in vivo study
point mutation
protein expression
reverse transcription polymerase chain reaction
RNA splicing
RNA translation
Western blotting
Adenoviridae
dc.description.none.fl_txt_mv Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.
Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Alonso, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata
url http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Mol. Cell 2003;12(2):525-532
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.711113

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