A polar mechanism coordinates different regions of alternative splicing within a single gene

Autores: Fededa, J.P.; Petrillo, E.; Gelfand, M.S.; Neverov, A.D.; Kadener, S.; Nogués, G.; Pelisch, F.; Baralle, F.E.; Muro, A.F.; Kornblihtt, A.R.
Año de publicación: 2005
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Alternative splicing plays a key role in generating protein diversity. Transfections with minigenes revealed coordination between two distant, alternatively spliced exons in the same gene. Mutations that either inhibit or stimulate inclusion of the upstream alternative exon deeply affect inclusion of the downstream one. However, similar mutations at the downstream alternative exon have little effect on the upstream one. This polar effect is promoter specific and is enhanced by inhibition of transcriptional elongation. Consistently, cells from mutant mice with either constitutive or null inclusion of a fibronectin alternative exon revealed coordination with a second alternative splicing region, located far downstream. Using allele-specific RT-PCR, we demonstrate that this coordination occurs in cis and is also affected by transcriptional elongation rates. Bioinformatics supports the generality of these findings, indicating that 25% of human genes contain multiple alternative splicing regions and identifying several genes with nonrandom distribution of mRNA isoforms at two alternative regions. Copyright ©2005 by Elsevier Inc.
Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Nogués, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Baralle, F.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente: Mol. Cell 2005;19(3):393-404
Materia: fibronectin
alternative RNA splicing
article
bioinformatics
cis isomer
gene identification
human
nonhuman
transcription regulation
Alleles
Alpha-Globulins
Alternative Splicing
Animals
Antigens, Viral, Tumor
Cell Line, Tumor
Cercopithecus aethiops
Computational Biology
COS Cells
Dichlororibofuranosylbenzimidazole
DNA-Binding Proteins
Exons
Fibroblasts
Fibronectins
Genes
Humans
Mice
Mice, Knockout
Models, Genetic
Nuclear Proteins
Promoter Regions (Genetics)
Protein Isoforms
RNA Polymerase II
RNA Splicing
RNA-Binding Proteins
Transcription Factors
Transfection
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador: paperaa:paper_10972765_v19_n3_p393_Fededa

Acceder

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network_name_str	Biblioteca Digital (UBA-FCEN)
spelling	A polar mechanism coordinates different regions of alternative splicing within a single geneFededa, J.P.Petrillo, E.Gelfand, M.S.Neverov, A.D.Kadener, S.Nogués, G.Pelisch, F.Baralle, F.E.Muro, A.F.Kornblihtt, A.R.fibronectinalternative RNA splicingarticlebioinformaticscis isomergene identificationhumannonhumantranscription regulationAllelesAlpha-GlobulinsAlternative SplicingAnimalsAntigens, Viral, TumorCell Line, TumorCercopithecus aethiopsComputational BiologyCOS CellsDichlororibofuranosylbenzimidazoleDNA-Binding ProteinsExonsFibroblastsFibronectinsGenesHumansMiceMice, KnockoutModels, GeneticNuclear ProteinsPromoter Regions (Genetics)Protein IsoformsRNA Polymerase IIRNA SplicingRNA-Binding ProteinsTranscription FactorsTransfectionAlternative splicing plays a key role in generating protein diversity. Transfections with minigenes revealed coordination between two distant, alternatively spliced exons in the same gene. Mutations that either inhibit or stimulate inclusion of the upstream alternative exon deeply affect inclusion of the downstream one. However, similar mutations at the downstream alternative exon have little effect on the upstream one. This polar effect is promoter specific and is enhanced by inhibition of transcriptional elongation. Consistently, cells from mutant mice with either constitutive or null inclusion of a fibronectin alternative exon revealed coordination with a second alternative splicing region, located far downstream. Using allele-specific RT-PCR, we demonstrate that this coordination occurs in cis and is also affected by transcriptional elongation rates. Bioinformatics supports the generality of these findings, indicating that 25% of human genes contain multiple alternative splicing regions and identifying several genes with nonrandom distribution of mRNA isoforms at two alternative regions. Copyright ©2005 by Elsevier Inc.Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Nogués, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Baralle, F.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_10972765_v19_n3_p393_FededaMol. Cell 2005;19(3):393-404reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:07Zpaperaa:paper_10972765_v19_n3_p393_FededaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:08.451Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv	A polar mechanism coordinates different regions of alternative splicing within a single gene
title	A polar mechanism coordinates different regions of alternative splicing within a single gene
spellingShingle	A polar mechanism coordinates different regions of alternative splicing within a single gene Fededa, J.P. fibronectin alternative RNA splicing article bioinformatics cis isomer gene identification human nonhuman transcription regulation Alleles Alpha-Globulins Alternative Splicing Animals Antigens, Viral, Tumor Cell Line, Tumor Cercopithecus aethiops Computational Biology COS Cells Dichlororibofuranosylbenzimidazole DNA-Binding Proteins Exons Fibroblasts Fibronectins Genes Humans Mice Mice, Knockout Models, Genetic Nuclear Proteins Promoter Regions (Genetics) Protein Isoforms RNA Polymerase II RNA Splicing RNA-Binding Proteins Transcription Factors Transfection
title_short	A polar mechanism coordinates different regions of alternative splicing within a single gene
title_full	A polar mechanism coordinates different regions of alternative splicing within a single gene
title_fullStr	A polar mechanism coordinates different regions of alternative splicing within a single gene
title_full_unstemmed	A polar mechanism coordinates different regions of alternative splicing within a single gene
title_sort	A polar mechanism coordinates different regions of alternative splicing within a single gene
dc.creator.none.fl_str_mv	Fededa, J.P. Petrillo, E. Gelfand, M.S. Neverov, A.D. Kadener, S. Nogués, G. Pelisch, F. Baralle, F.E. Muro, A.F. Kornblihtt, A.R.
author	Fededa, J.P.
author_facet	Fededa, J.P. Petrillo, E. Gelfand, M.S. Neverov, A.D. Kadener, S. Nogués, G. Pelisch, F. Baralle, F.E. Muro, A.F. Kornblihtt, A.R.
author_role	author
author2	Petrillo, E. Gelfand, M.S. Neverov, A.D. Kadener, S. Nogués, G. Pelisch, F. Baralle, F.E. Muro, A.F. Kornblihtt, A.R.
author2_role	author author author author author author author author author
dc.subject.none.fl_str_mv	fibronectin alternative RNA splicing article bioinformatics cis isomer gene identification human nonhuman transcription regulation Alleles Alpha-Globulins Alternative Splicing Animals Antigens, Viral, Tumor Cell Line, Tumor Cercopithecus aethiops Computational Biology COS Cells Dichlororibofuranosylbenzimidazole DNA-Binding Proteins Exons Fibroblasts Fibronectins Genes Humans Mice Mice, Knockout Models, Genetic Nuclear Proteins Promoter Regions (Genetics) Protein Isoforms RNA Polymerase II RNA Splicing RNA-Binding Proteins Transcription Factors Transfection
topic	fibronectin alternative RNA splicing article bioinformatics cis isomer gene identification human nonhuman transcription regulation Alleles Alpha-Globulins Alternative Splicing Animals Antigens, Viral, Tumor Cell Line, Tumor Cercopithecus aethiops Computational Biology COS Cells Dichlororibofuranosylbenzimidazole DNA-Binding Proteins Exons Fibroblasts Fibronectins Genes Humans Mice Mice, Knockout Models, Genetic Nuclear Proteins Promoter Regions (Genetics) Protein Isoforms RNA Polymerase II RNA Splicing RNA-Binding Proteins Transcription Factors Transfection
dc.description.none.fl_txt_mv	Alternative splicing plays a key role in generating protein diversity. Transfections with minigenes revealed coordination between two distant, alternatively spliced exons in the same gene. Mutations that either inhibit or stimulate inclusion of the upstream alternative exon deeply affect inclusion of the downstream one. However, similar mutations at the downstream alternative exon have little effect on the upstream one. This polar effect is promoter specific and is enhanced by inhibition of transcriptional elongation. Consistently, cells from mutant mice with either constitutive or null inclusion of a fibronectin alternative exon revealed coordination with a second alternative splicing region, located far downstream. Using allele-specific RT-PCR, we demonstrate that this coordination occurs in cis and is also affected by transcriptional elongation rates. Bioinformatics supports the generality of these findings, indicating that 25% of human genes contain multiple alternative splicing regions and identifying several genes with nonrandom distribution of mRNA isoforms at two alternative regions. Copyright ©2005 by Elsevier Inc. Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nogués, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baralle, F.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description	Alternative splicing plays a key role in generating protein diversity. Transfections with minigenes revealed coordination between two distant, alternatively spliced exons in the same gene. Mutations that either inhibit or stimulate inclusion of the upstream alternative exon deeply affect inclusion of the downstream one. However, similar mutations at the downstream alternative exon have little effect on the upstream one. This polar effect is promoter specific and is enhanced by inhibition of transcriptional elongation. Consistently, cells from mutant mice with either constitutive or null inclusion of a fibronectin alternative exon revealed coordination with a second alternative splicing region, located far downstream. Using allele-specific RT-PCR, we demonstrate that this coordination occurs in cis and is also affected by transcriptional elongation rates. Bioinformatics supports the generality of these findings, indicating that 25% of human genes contain multiple alternative splicing regions and identifying several genes with nonrandom distribution of mRNA isoforms at two alternative regions. Copyright ©2005 by Elsevier Inc.
publishDate	2005
dc.date.none.fl_str_mv	2005
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12110/paper_10972765_v19_n3_p393_Fededa
url	http://hdl.handle.net/20.500.12110/paper_10972765_v19_n3_p393_Fededa
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	Mol. Cell 2005;19(3):393-404 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN
reponame_str	Biblioteca Digital (UBA-FCEN)
collection	Biblioteca Digital (UBA-FCEN)
instname_str	Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str	UBA-FCEN
institution	UBA-FCEN
repository.name.fl_str_mv	Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
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A polar mechanism coordinates different regions of alternative splicing within a single gene

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