Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina

Autores
Bailat, Alejandra Susana
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
tesis doctoral
Estado
versión aceptada
Colaborador/a o director/a de tesis
Malan Borel, Ileana Sofía del Carmen
Álvarez, Élida María
Gimeno, Eduardo Juan
Trono, Karina Gabriela
Descripción
Fil: Bailat, Alejandra Susana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
El Virus de la Anemia Infecciosa Equina (VAIE) ocasiona una enfermedad persistente en equinos, que culmina en una prolongada fase asintomática, evidenciando un estado de equilibrio permanente entre la infección viral y el sistema inmune del animal. A fin de analizar la participación de receptores y citoquinas en la respuesta inmune de equinos que cursan esta etapa de la infección, se investigó, mediante transcripción reversa seguida de PCR semicuantitativa, la expresión de receptores y citoquinas en células mononucleares de sangre periférica (PBMC). Por otra parte, mediante inmunohistoquímica se estudió la expresión de dichas moléculas en hígado y bazo, órganos considerados reservorio viral. Se emplearon, además, estudios de estimulación in vitro de células dendríticas para evaluar la capacidad de los receptores de la familia TLRs, de reconocer secuencias conservadas del VAIE y finalmente se estudió la secreción de citoquinas y la expresión de receptores en PBMC, en respuesta al estímulo con secuencias virales conservadas. Los resultados mostraron niveles basales de IFN-alfa disminuidos en PBMC de equinos VAIE(+), mientras que en órganos reservorio presentan niveles disminuidos de IFN-gama, sugiriendo la presencia de mecanismos regulatorios que limitan la expresión de citoquinas antivirales. Los estudios de estimulación in vitro mostraron grandes variaciones en los perfiles de citoquinas entre los equinos asintomáticos. Estos resultados evidencian la complejidad del estudio de infecciones lentivirales en especies no endocriadas y concuerdan con investigaciones realizadas en otras infecciones retrovirales, respecto de los perfiles de citoquinas, la variabilidad entre individuos y su relación con la progresión de la enfermedad.
The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.
Consejo Nacional de Investigaciones Científicas y Técnicas
Universidad Nacional del Litoral
Materia
Virus de la Anemia Infecciosa Equina
Respuesta inmune
Citoquinas
Receptores celulares
Retrovirus
Equine Infectious Anemia Virus
Immune response
Cytokines
Cellular receptors
Retrovirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
Repositorio
Biblioteca Virtual (UNL)
Institución
Universidad Nacional del Litoral
OAI Identificador
oai:https://bibliotecavirtual.unl.edu.ar:11185/6374

id UNLBT_f0f55b69615a694fa4c7de257b574cb7
oai_identifier_str oai:https://bibliotecavirtual.unl.edu.ar:11185/6374
network_acronym_str UNLBT
repository_id_str 2187
network_name_str Biblioteca Virtual (UNL)
spelling Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa EquinaStudy of the innate and acquired immune responses in horses infected with Equine Infectious Anemia VirusBailat, Alejandra SusanaVirus de la Anemia Infecciosa EquinaRespuesta inmuneCitoquinasReceptores celularesRetrovirusEquine Infectious Anemia VirusImmune responseCytokinesCellular receptorsRetrovirusFil: Bailat, Alejandra Susana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.El Virus de la Anemia Infecciosa Equina (VAIE) ocasiona una enfermedad persistente en equinos, que culmina en una prolongada fase asintomática, evidenciando un estado de equilibrio permanente entre la infección viral y el sistema inmune del animal. A fin de analizar la participación de receptores y citoquinas en la respuesta inmune de equinos que cursan esta etapa de la infección, se investigó, mediante transcripción reversa seguida de PCR semicuantitativa, la expresión de receptores y citoquinas en células mononucleares de sangre periférica (PBMC). Por otra parte, mediante inmunohistoquímica se estudió la expresión de dichas moléculas en hígado y bazo, órganos considerados reservorio viral. Se emplearon, además, estudios de estimulación in vitro de células dendríticas para evaluar la capacidad de los receptores de la familia TLRs, de reconocer secuencias conservadas del VAIE y finalmente se estudió la secreción de citoquinas y la expresión de receptores en PBMC, en respuesta al estímulo con secuencias virales conservadas. Los resultados mostraron niveles basales de IFN-alfa disminuidos en PBMC de equinos VAIE(+), mientras que en órganos reservorio presentan niveles disminuidos de IFN-gama, sugiriendo la presencia de mecanismos regulatorios que limitan la expresión de citoquinas antivirales. Los estudios de estimulación in vitro mostraron grandes variaciones en los perfiles de citoquinas entre los equinos asintomáticos. Estos resultados evidencian la complejidad del estudio de infecciones lentivirales en especies no endocriadas y concuerdan con investigaciones realizadas en otras infecciones retrovirales, respecto de los perfiles de citoquinas, la variabilidad entre individuos y su relación con la progresión de la enfermedad.The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.Consejo Nacional de Investigaciones Científicas y TécnicasUniversidad Nacional del LitoralMalan Borel, Ileana Sofía del CarmenÁlvarez, Élida MaríaGimeno, Eduardo JuanTrono, Karina Gabriela2022-03-28T11:44:15Z2013-03-01SNRDinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttps://hdl.handle.net/11185/6374spainfo:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://creativecommons.org/licenses/by-nc-nd/4.0/deed.esreponame:Biblioteca Virtual (UNL)instname:Universidad Nacional del Litoralinstacron:UNL2025-09-29T14:30:38Zoai:https://bibliotecavirtual.unl.edu.ar:11185/6374Institucionalhttp://bibliotecavirtual.unl.edu.ar/Universidad públicaNo correspondeajdeba@unl.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:21872025-09-29 14:30:39.328Biblioteca Virtual (UNL) - Universidad Nacional del Litoralfalse
dc.title.none.fl_str_mv Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
Study of the innate and acquired immune responses in horses infected with Equine Infectious Anemia Virus
title Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
spellingShingle Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
Bailat, Alejandra Susana
Virus de la Anemia Infecciosa Equina
Respuesta inmune
Citoquinas
Receptores celulares
Retrovirus
Equine Infectious Anemia Virus
Immune response
Cytokines
Cellular receptors
Retrovirus
title_short Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
title_full Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
title_fullStr Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
title_full_unstemmed Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
title_sort Estudio de la respuesta inmune, innata y adquirida, en equinos infectados con el Virus de la Anemia Infecciosa Equina
dc.creator.none.fl_str_mv Bailat, Alejandra Susana
author Bailat, Alejandra Susana
author_facet Bailat, Alejandra Susana
author_role author
dc.contributor.none.fl_str_mv Malan Borel, Ileana Sofía del Carmen
Álvarez, Élida María
Gimeno, Eduardo Juan
Trono, Karina Gabriela
dc.subject.none.fl_str_mv Virus de la Anemia Infecciosa Equina
Respuesta inmune
Citoquinas
Receptores celulares
Retrovirus
Equine Infectious Anemia Virus
Immune response
Cytokines
Cellular receptors
Retrovirus
topic Virus de la Anemia Infecciosa Equina
Respuesta inmune
Citoquinas
Receptores celulares
Retrovirus
Equine Infectious Anemia Virus
Immune response
Cytokines
Cellular receptors
Retrovirus
dc.description.none.fl_txt_mv Fil: Bailat, Alejandra Susana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
El Virus de la Anemia Infecciosa Equina (VAIE) ocasiona una enfermedad persistente en equinos, que culmina en una prolongada fase asintomática, evidenciando un estado de equilibrio permanente entre la infección viral y el sistema inmune del animal. A fin de analizar la participación de receptores y citoquinas en la respuesta inmune de equinos que cursan esta etapa de la infección, se investigó, mediante transcripción reversa seguida de PCR semicuantitativa, la expresión de receptores y citoquinas en células mononucleares de sangre periférica (PBMC). Por otra parte, mediante inmunohistoquímica se estudió la expresión de dichas moléculas en hígado y bazo, órganos considerados reservorio viral. Se emplearon, además, estudios de estimulación in vitro de células dendríticas para evaluar la capacidad de los receptores de la familia TLRs, de reconocer secuencias conservadas del VAIE y finalmente se estudió la secreción de citoquinas y la expresión de receptores en PBMC, en respuesta al estímulo con secuencias virales conservadas. Los resultados mostraron niveles basales de IFN-alfa disminuidos en PBMC de equinos VAIE(+), mientras que en órganos reservorio presentan niveles disminuidos de IFN-gama, sugiriendo la presencia de mecanismos regulatorios que limitan la expresión de citoquinas antivirales. Los estudios de estimulación in vitro mostraron grandes variaciones en los perfiles de citoquinas entre los equinos asintomáticos. Estos resultados evidencian la complejidad del estudio de infecciones lentivirales en especies no endocriadas y concuerdan con investigaciones realizadas en otras infecciones retrovirales, respecto de los perfiles de citoquinas, la variabilidad entre individuos y su relación con la progresión de la enfermedad.
The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.The Equine Infectious Anemia Virus (EIAV) causes persistent disease in horses that is characterized by lethargy and chronic anemia. Equine Infectious Anemia (EIA) has a cyclical and dynamic evolution, in which the animals remain free of clinical symptoms but become life-long EIAV carriers. A key role in controlling viral replication at this stage of infection was assigned to the immune system. To examine the role of receptors and cytokines in horse's immune response during this stage of infection, reverse transcription followed by semiquantitative PCR was performed in peripheral blood mononuclear cells (PBMC). Moreover, the expression of such molecules in viral reservoir organs such liver and spleen, was studied by immunohistochemistry. On the other hand, in vitro stimulation of dendritic cells was done in order to evaluate the ability of Toll-Like Receptors family, to recognize conserved sequences of EIAV. Finally, cytokines and receptor expression in PBMC in response to specific stimulation with conserved viral sequences was evaluated. The results showed decreased levels of IFN-alpha in PBMC of EIAV(+) horses, while decreased levels of IFN-gamma in reservoir organs were found, suggesting the presence of regulatory mechanisms that limit the expression of antiviral cytokines. PBMC in vitro stimulation studies showed large variations in cytokine profiles among asymptomatic equines. These results show the complexity of the study of lentiviral infections in not inbred species and are consistent with research conducted in other retroviral infections, regarding cytokine profiles, variability between individuals and their relationship to disease progression.
Consejo Nacional de Investigaciones Científicas y Técnicas
Universidad Nacional del Litoral
description Fil: Bailat, Alejandra Susana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
2022-03-28T11:44:15Z
dc.type.none.fl_str_mv SNRD
info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_db06
info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11185/6374
url https://hdl.handle.net/11185/6374
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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instname_str Universidad Nacional del Litoral
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