Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites

Autores
Tran, Tung; Andres, Patricia; Petroni, Alejandro; Soler Bistué, Alfonso J. C.; Albornoz, Ezequiel; Zorreguieta, Angeles; Reyes-Lamothe, Rodrigo; Sherratt, David J; Corso, Alejandra; Tolmasky, Marcelo E.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Tran, Tung. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.
Fil: Andres, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Soler-Bistué, Alfonso. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; Argentina
Fil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Zorreguieta, Angeles. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; Argentina Fil: Reyes-Lamothe, Rodrigo. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.
Fil: Sherratt, David J. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Tolmasky, Marcelo E. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.
Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
Fuente
Antimicrobial Agents and Chemotherapy 2012; 56(4):1821-1827
Materia
Escherichia coli
Salmonella
Plásmidos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1795
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/1795
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sitesTran, TungAndres, PatriciaPetroni, AlejandroSoler Bistué, Alfonso J. C.Albornoz, EzequielZorreguieta, AngelesReyes-Lamothe, RodrigoSherratt, David JCorso, AlejandraTolmasky, Marcelo E.Escherichia coliSalmonellaPlásmidosFil: Tran, Tung. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.Fil: Andres, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Soler-Bistué, Alfonso. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; ArgentinaFil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Zorreguieta, Angeles. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; Argentina Fil: Reyes-Lamothe, Rodrigo. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.Fil: Sherratt, David J. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Tolmasky, Marcelo E. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.American Society for Microbiology2012-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-6596http://sgc.anlis.gob.ar/handle/123456789/179510.1128/AAC.06036-11Antimicrobial Agents and Chemotherapy 2012; 56(4):1821-1827reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISAntimicrobial agents and chemotherapyenginfo:eu-repo/semantics/openAccess2025-09-11T10:51:25Zoai:sgc.anlis.gob.ar:Publications/123456789/1795Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-11 10:51:25.358Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
title Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
spellingShingle Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
Tran, Tung
Escherichia coli
Salmonella
Plásmidos
title_short Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
title_full Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
title_fullStr Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
title_full_unstemmed Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
title_sort Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites
dc.creator.none.fl_str_mv Tran, Tung
Andres, Patricia
Petroni, Alejandro
Soler Bistué, Alfonso J. C.
Albornoz, Ezequiel
Zorreguieta, Angeles
Reyes-Lamothe, Rodrigo
Sherratt, David J
Corso, Alejandra
Tolmasky, Marcelo E.
author Tran, Tung
author_facet Tran, Tung
Andres, Patricia
Petroni, Alejandro
Soler Bistué, Alfonso J. C.
Albornoz, Ezequiel
Zorreguieta, Angeles
Reyes-Lamothe, Rodrigo
Sherratt, David J
Corso, Alejandra
Tolmasky, Marcelo E.
author_role author
author2 Andres, Patricia
Petroni, Alejandro
Soler Bistué, Alfonso J. C.
Albornoz, Ezequiel
Zorreguieta, Angeles
Reyes-Lamothe, Rodrigo
Sherratt, David J
Corso, Alejandra
Tolmasky, Marcelo E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Salmonella
Plásmidos
topic Escherichia coli
Salmonella
Plásmidos
dc.description.none.fl_txt_mv Fil: Tran, Tung. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.
Fil: Andres, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Soler-Bistué, Alfonso. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; Argentina
Fil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Zorreguieta, Angeles. Fundación Instituto Leloir, CONICET, FCEyN, Universidad de Buenos Aires; Argentina Fil: Reyes-Lamothe, Rodrigo. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.
Fil: Sherratt, David J. Division of Molecular Genetics, Department of Biochemistry, University of Oxford; Reino Unido.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Tolmasky, Marcelo E. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.
Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
description Fil: Tran, Tung. Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton; Estados Unidos.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-6596
http://sgc.anlis.gob.ar/handle/123456789/1795
10.1128/AAC.06036-11
identifier_str_mv 1098-6596
10.1128/AAC.06036-11
url http://sgc.anlis.gob.ar/handle/123456789/1795
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antimicrobial agents and chemotherapy
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Antimicrobial Agents and Chemotherapy 2012; 56(4):1821-1827
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.993085

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