Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method

Autores
Rojas, Florencia D; Sosa, María de los A; Fernandez, Mariana; Cattana, María E; Córdoba, Susana; Giusiano, Gustavo E
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32ºC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
Materia
Dermatomicosis
Malassezia
Antifúngicos
Anfotericina B
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1913
Acceder
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network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution methodRojas, Florencia DSosa, María de los AFernandez, MarianaCattana, María ECórdoba, SusanaGiusiano, Gustavo EDermatomicosisMalasseziaAntifúngicosAnfotericina BFil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32ºC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.2014-08info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1460-2709http://sgc.anlis.gob.ar/handle/123456789/191310.1093/mmy/myu010Medical mycologyenginfo:eu-repo/semantics/openAccessreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS2025-09-04T11:17:52Zoai:sgc.anlis.gob.ar:Publications/123456789/1913Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:17:52.411Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
spellingShingle Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
Rojas, Florencia D
Dermatomicosis
Malassezia
Antifúngicos
Anfotericina B
title_short Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_fullStr Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full_unstemmed Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_sort Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
dc.creator.none.fl_str_mv Rojas, Florencia D
Sosa, María de los A
Fernandez, Mariana
Cattana, María E
Córdoba, Susana
Giusiano, Gustavo E
author Rojas, Florencia D
author_facet Rojas, Florencia D
Sosa, María de los A
Fernandez, Mariana
Cattana, María E
Córdoba, Susana
Giusiano, Gustavo E
author_role author
author2 Sosa, María de los A
Fernandez, Mariana
Cattana, María E
Córdoba, Susana
Giusiano, Gustavo E
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Dermatomicosis
Malassezia
Antifúngicos
Anfotericina B
topic Dermatomicosis
Malassezia
Antifúngicos
Anfotericina B
dc.description.none.fl_txt_mv Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32ºC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
description Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1460-2709
http://sgc.anlis.gob.ar/handle/123456789/1913
10.1093/mmy/myu010
identifier_str_mv 1460-2709
10.1093/mmy/myu010
url http://sgc.anlis.gob.ar/handle/123456789/1913
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medical mycology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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