Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method

Autores
Rojas, Florencia Dinorah; Sosa, Maria de Los Angeles; Fernández, Mariana Soledad; Cattana, Maria Emilia; Córdoba, Susana Beatríz; Giusiano, Gustavo Emilio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, andM. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile,malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sosa, Maria de Los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Fernández, Mariana Soledad. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cattana, Maria Emilia. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Córdoba, Susana Beatríz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Malassezia
Susceptibility
Broth Microdilution
Azole Drug
Amphotericin B.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28148
Acceder
id CONICETDig_1c7ba020addc767a8d04be11bb94bcb5
oai_identifier_str oai:ri.conicet.gov.ar:11336/28148
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution methodRojas, Florencia DinorahSosa, Maria de Los AngelesFernández, Mariana SoledadCattana, Maria EmiliaCórdoba, Susana BeatrízGiusiano, Gustavo EmilioMalasseziaSusceptibilityBroth MicrodilutionAzole DrugAmphotericin B.https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, andM. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile,malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sosa, Maria de Los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Fernández, Mariana Soledad. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cattana, Maria Emilia. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Córdoba, Susana Beatríz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis Ltd2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28148Rojas, Florencia Dinorah; Sosa, Maria de Los Angeles; Fernández, Mariana Soledad; Cattana, Maria Emilia; Córdoba, Susana Beatríz; et al.; Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method; Taylor & Francis Ltd; Medical Mycology; 52; 6; 8-2014; 641-6461369-37861460-2709CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myu010info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/article/52/6/641/2802489info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:23Zoai:ri.conicet.gov.ar:11336/28148instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:23.954CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
spellingShingle Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
Rojas, Florencia Dinorah
Malassezia
Susceptibility
Broth Microdilution
Azole Drug
Amphotericin B.
title_short Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_fullStr Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full_unstemmed Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_sort Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
dc.creator.none.fl_str_mv Rojas, Florencia Dinorah
Sosa, Maria de Los Angeles
Fernández, Mariana Soledad
Cattana, Maria Emilia
Córdoba, Susana Beatríz
Giusiano, Gustavo Emilio
author Rojas, Florencia Dinorah
author_facet Rojas, Florencia Dinorah
Sosa, Maria de Los Angeles
Fernández, Mariana Soledad
Cattana, Maria Emilia
Córdoba, Susana Beatríz
Giusiano, Gustavo Emilio
author_role author
author2 Sosa, Maria de Los Angeles
Fernández, Mariana Soledad
Cattana, Maria Emilia
Córdoba, Susana Beatríz
Giusiano, Gustavo Emilio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Malassezia
Susceptibility
Broth Microdilution
Azole Drug
Amphotericin B.
topic Malassezia
Susceptibility
Broth Microdilution
Azole Drug
Amphotericin B.
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, andM. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile,malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
Fil: Rojas, Florencia Dinorah. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sosa, Maria de Los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Fernández, Mariana Soledad. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cattana, Maria Emilia. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Córdoba, Susana Beatríz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, andM. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile,malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28148
Rojas, Florencia Dinorah; Sosa, Maria de Los Angeles; Fernández, Mariana Soledad; Cattana, Maria Emilia; Córdoba, Susana Beatríz; et al.; Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method; Taylor & Francis Ltd; Medical Mycology; 52; 6; 8-2014; 641-646
1369-3786
1460-2709
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28148
identifier_str_mv Rojas, Florencia Dinorah; Sosa, Maria de Los Angeles; Fernández, Mariana Soledad; Cattana, Maria Emilia; Córdoba, Susana Beatríz; et al.; Antifungal susceptibility of malassezia furfur, malassezia sympodialis and malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method; Taylor & Francis Ltd; Medical Mycology; 52; 6; 8-2014; 641-646
1369-3786
1460-2709
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myu010
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/article/52/6/641/2802489
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269283045867520
score 13.13397

Publicaciones similares