Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment

Autores
Yokobori, Noemi; López, Beatriz; Monteserin, Johana; Paul, Roxana; von Groll, Andrea; Martin, Anandi; Marquina-Castillo, Brenda; Palomino, Juan Carlos; Hernández-Pando, Rogelio; Sasiain, María Del Carmen; Ritacco, Viviana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil.
Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica.
Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica.
Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success.
Fuente
International Journal of Medical Microbiology 2018;308(3):349-347
Materia
Aptitud Genética
Tuberculosis Resistente a Múltiples Medicamentos
Mycobacterium tuberculosis
Virulencia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/2214

id SGCANLIS_a44ce2e2283bb49a62d80c05bdfb8fb3
oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/2214
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessmentYokobori, NoemiLópez, BeatrizMonteserin, JohanaPaul, Roxanavon Groll, AndreaMartin, AnandiMarquina-Castillo, BrendaPalomino, Juan CarlosHernández-Pando, RogelioSasiain, María Del CarmenRitacco, VivianaAptitud GenéticaTuberculosis Resistente a Múltiples MedicamentosMycobacterium tuberculosisVirulenciaFil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil.Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica.Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica.Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success.2018-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1618-0607http://sgc.anlis.gob.ar/handle/123456789/221410.1016/j.ijmm.2018.01.006International Journal of Medical Microbiology 2018;308(3):349-347reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInternational journal of medical microbiology : IJMMnoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:43Zoai:sgc.anlis.gob.ar:Publications/123456789/2214Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:43.551Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
title Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
spellingShingle Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
Yokobori, Noemi
Aptitud Genética
Tuberculosis Resistente a Múltiples Medicamentos
Mycobacterium tuberculosis
Virulencia
title_short Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
title_full Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
title_fullStr Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
title_full_unstemmed Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
title_sort Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
dc.creator.none.fl_str_mv Yokobori, Noemi
López, Beatriz
Monteserin, Johana
Paul, Roxana
von Groll, Andrea
Martin, Anandi
Marquina-Castillo, Brenda
Palomino, Juan Carlos
Hernández-Pando, Rogelio
Sasiain, María Del Carmen
Ritacco, Viviana
author Yokobori, Noemi
author_facet Yokobori, Noemi
López, Beatriz
Monteserin, Johana
Paul, Roxana
von Groll, Andrea
Martin, Anandi
Marquina-Castillo, Brenda
Palomino, Juan Carlos
Hernández-Pando, Rogelio
Sasiain, María Del Carmen
Ritacco, Viviana
author_role author
author2 López, Beatriz
Monteserin, Johana
Paul, Roxana
von Groll, Andrea
Martin, Anandi
Marquina-Castillo, Brenda
Palomino, Juan Carlos
Hernández-Pando, Rogelio
Sasiain, María Del Carmen
Ritacco, Viviana
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aptitud Genética
Tuberculosis Resistente a Múltiples Medicamentos
Mycobacterium tuberculosis
Virulencia
topic Aptitud Genética
Tuberculosis Resistente a Múltiples Medicamentos
Mycobacterium tuberculosis
Virulencia
dc.description.none.fl_txt_mv Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil.
Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica.
Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica.
Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success.
description Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1618-0607
http://sgc.anlis.gob.ar/handle/123456789/2214
10.1016/j.ijmm.2018.01.006
identifier_str_mv 1618-0607
10.1016/j.ijmm.2018.01.006
url http://sgc.anlis.gob.ar/handle/123456789/2214
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International journal of medical microbiology : IJMM
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv International Journal of Medical Microbiology 2018;308(3):349-347
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
_version_ 1844621859580018688
score 12.559606