Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
- Autores
- Yokobori, Noemi; López, Beatriz; Monteserin, Johana; Paul, Roxana; von Groll, Andrea; Martin, Anandi; Marquina-Castillo, Brenda; Palomino, Juan Carlos; Hernández-Pando, Rogelio; Sasiain, María Del Carmen; Ritacco, Viviana
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil.
Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica.
Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica.
Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.
Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.
Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success. - Fuente
- International Journal of Medical Microbiology 2018;308(3):349-347
- Materia
-
Aptitud Genética
Tuberculosis Resistente a Múltiples Medicamentos
Mycobacterium tuberculosis
Virulencia - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- none
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/2214
Ver los metadatos del registro completo
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Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessmentYokobori, NoemiLópez, BeatrizMonteserin, JohanaPaul, Roxanavon Groll, AndreaMartin, AnandiMarquina-Castillo, BrendaPalomino, Juan CarlosHernández-Pando, RogelioSasiain, María Del CarmenRitacco, VivianaAptitud GenéticaTuberculosis Resistente a Múltiples MedicamentosMycobacterium tuberculosisVirulenciaFil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil.Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica.Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica.Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México.Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina.Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina.Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success.2018-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1618-0607http://sgc.anlis.gob.ar/handle/123456789/221410.1016/j.ijmm.2018.01.006International Journal of Medical Microbiology 2018;308(3):349-347reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInternational journal of medical microbiology : IJMMnoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:43Zoai:sgc.anlis.gob.ar:123456789/2214Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:43.548Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
title |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
spellingShingle |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment Yokobori, Noemi Aptitud Genética Tuberculosis Resistente a Múltiples Medicamentos Mycobacterium tuberculosis Virulencia |
title_short |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
title_full |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
title_fullStr |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
title_full_unstemmed |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
title_sort |
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment |
dc.creator.none.fl_str_mv |
Yokobori, Noemi López, Beatriz Monteserin, Johana Paul, Roxana von Groll, Andrea Martin, Anandi Marquina-Castillo, Brenda Palomino, Juan Carlos Hernández-Pando, Rogelio Sasiain, María Del Carmen Ritacco, Viviana |
author |
Yokobori, Noemi |
author_facet |
Yokobori, Noemi López, Beatriz Monteserin, Johana Paul, Roxana von Groll, Andrea Martin, Anandi Marquina-Castillo, Brenda Palomino, Juan Carlos Hernández-Pando, Rogelio Sasiain, María Del Carmen Ritacco, Viviana |
author_role |
author |
author2 |
López, Beatriz Monteserin, Johana Paul, Roxana von Groll, Andrea Martin, Anandi Marquina-Castillo, Brenda Palomino, Juan Carlos Hernández-Pando, Rogelio Sasiain, María Del Carmen Ritacco, Viviana |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Aptitud Genética Tuberculosis Resistente a Múltiples Medicamentos Mycobacterium tuberculosis Virulencia |
topic |
Aptitud Genética Tuberculosis Resistente a Múltiples Medicamentos Mycobacterium tuberculosis Virulencia |
dc.description.none.fl_txt_mv |
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina. Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Monteserin, Johana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina. Fil: Paul, Roxana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Von Groll, Andrea. Universidade Federal do Rio Grande. Faculty of Medicine. Medical Microbiology Research Center, Porto Alegre, Río Grande del Sur; Brasil. Fil: Martin, Anandi. Université Catholique de Louvain. Institute of Experimental and Clinical Research. Pôle of Medical Microbiology, Bruselas; Bélgica. Fil: Marquina-Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México. Fil: Palomino, Juan Carlos. Ghent University. Laboratory of Microbiology, Gante; Bélgica. Fil: Hernández-Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Sección de Patología Experimental, Ciudad de México; México. Fil: Sasiain, María Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina. Fil: Ritacco, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires; Argentina. Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success. |
description |
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX), Buenos Aires; Argentina. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1618-0607 http://sgc.anlis.gob.ar/handle/123456789/2214 10.1016/j.ijmm.2018.01.006 |
identifier_str_mv |
1618-0607 10.1016/j.ijmm.2018.01.006 |
url |
http://sgc.anlis.gob.ar/handle/123456789/2214 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International journal of medical microbiology : IJMM |
dc.rights.none.fl_str_mv |
none info:eu-repo/semantics/openAccess |
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none |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
International Journal of Medical Microbiology 2018;308(3):349-347 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
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ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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