Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models

Autores
Yokobori, Noemi; López, Beatriz; Geffner, Laura; Sabio y García, Carmen; Schierloh, Pablo; Barrera, Lucía; de la Barrera, Silvia; Sakai, Shunsuke; Kawamura, Ikuo; Mitsuyama, Masao; Ritacco, Viviana; Sasiain, María Del Carmen
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Yokobori, Noemi. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Geffner, Laura. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Sabio y García, Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Schierloh, Pablo. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: de la Barrera, Silvia. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Sakai, Shunsuke. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Kawamura, Ikuo. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Mitsuyama, Masao. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Sasiain, María Del Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Mycobacterium tuberculosis has a considerable degree of genetic variability resulting in different epidemiology and disease outcomes. We evaluated the pathogen-host cell interaction of two genetically closely-related multidrug-resistant M. tuberculosis strains of the Haarlem family, namely the strain M, responsible for an extensive multidrug-resistant tuberculosis outbreak, and its kin strain 410 which caused a single case in two decades. Intracellular growth and cytokine responses were evaluated in human monocyte-derived macrophages and dU937 macrophage-like cells. In monocyte-derived macrophages, strain M grew more slowly and induced lower levels of TNF-α and IL-10 than 410, contrasting with previous studies with other strains, where a direct correlation was observed between increased intracellular growth and epidemiological success. On the other hand, in dU937 cells, no difference in growth was observed between both strains, and strain M induced significantly higher TNF-α levels than strain 410. We found that both cell models differed critically in the expression of receptors for M. tuberculosis entry, which might explain the different infection outcomes. Our results in monocyte-derived macrophages suggest that strain M relies on a modest replication rate and cytokine induction, keeping a state of quiescence and remaining rather unnoticed by the host. Collectively, our results underscore the impact of M. tuberculosis intra-species variations on the outcome of host cell infection and show that results can differ depending on the in vitro infection model.
Fuente
Infection, Genetics and Evolution 2013; 16:151-6.
Materia
Resistencia a Múltiples Medicamentos
Mycobacterium tuberculosis
Macrófagos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/2048
Acceder
id SGCANLIS_45a9f2403a366f133c2ecd4226f4fab8
oai_identifier_str oai:sgc.anlis.gob.ar:123456789/2048
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage modelsYokobori, NoemiLópez, BeatrizGeffner, LauraSabio y García, CarmenSchierloh, PabloBarrera, Lucíade la Barrera, SilviaSakai, ShunsukeKawamura, IkuoMitsuyama, MasaoRitacco, VivianaSasiain, María Del CarmenResistencia a Múltiples MedicamentosMycobacterium tuberculosisMacrófagosFil: Yokobori, Noemi. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Geffner, Laura. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Sabio y García, Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Schierloh, Pablo. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: de la Barrera, Silvia. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Sakai, Shunsuke. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.Fil: Kawamura, Ikuo. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.Fil: Mitsuyama, Masao. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Sasiain, María Del Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.Mycobacterium tuberculosis has a considerable degree of genetic variability resulting in different epidemiology and disease outcomes. We evaluated the pathogen-host cell interaction of two genetically closely-related multidrug-resistant M. tuberculosis strains of the Haarlem family, namely the strain M, responsible for an extensive multidrug-resistant tuberculosis outbreak, and its kin strain 410 which caused a single case in two decades. Intracellular growth and cytokine responses were evaluated in human monocyte-derived macrophages and dU937 macrophage-like cells. In monocyte-derived macrophages, strain M grew more slowly and induced lower levels of TNF-α and IL-10 than 410, contrasting with previous studies with other strains, where a direct correlation was observed between increased intracellular growth and epidemiological success. On the other hand, in dU937 cells, no difference in growth was observed between both strains, and strain M induced significantly higher TNF-α levels than strain 410. We found that both cell models differed critically in the expression of receptors for M. tuberculosis entry, which might explain the different infection outcomes. Our results in monocyte-derived macrophages suggest that strain M relies on a modest replication rate and cytokine induction, keeping a state of quiescence and remaining rather unnoticed by the host. Collectively, our results underscore the impact of M. tuberculosis intra-species variations on the outcome of host cell infection and show that results can differ depending on the in vitro infection model.Elsevier2013-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1567-1348https://www.sciencedirect.com/science/article/abs/pii/S1567134813000245?via%3Dihubhttp://sgc.anlis.gob.ar/handle/123456789/204810.1016/j.meegid.2013.01.007Infection, Genetics and Evolution 2013; 16:151-6.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseasesnoneinfo:eu-repo/semantics/openAccesseng2025-10-23T11:20:48Zoai:sgc.anlis.gob.ar:123456789/2048Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-10-23 11:20:48.953Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
title Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
spellingShingle Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
Yokobori, Noemi
Resistencia a Múltiples Medicamentos
Mycobacterium tuberculosis
Macrófagos
title_short Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
title_full Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
title_fullStr Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
title_full_unstemmed Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
title_sort Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
dc.creator.none.fl_str_mv Yokobori, Noemi
López, Beatriz
Geffner, Laura
Sabio y García, Carmen
Schierloh, Pablo
Barrera, Lucía
de la Barrera, Silvia
Sakai, Shunsuke
Kawamura, Ikuo
Mitsuyama, Masao
Ritacco, Viviana
Sasiain, María Del Carmen
author Yokobori, Noemi
author_facet Yokobori, Noemi
López, Beatriz
Geffner, Laura
Sabio y García, Carmen
Schierloh, Pablo
Barrera, Lucía
de la Barrera, Silvia
Sakai, Shunsuke
Kawamura, Ikuo
Mitsuyama, Masao
Ritacco, Viviana
Sasiain, María Del Carmen
author_role author
author2 López, Beatriz
Geffner, Laura
Sabio y García, Carmen
Schierloh, Pablo
Barrera, Lucía
de la Barrera, Silvia
Sakai, Shunsuke
Kawamura, Ikuo
Mitsuyama, Masao
Ritacco, Viviana
Sasiain, María Del Carmen
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Resistencia a Múltiples Medicamentos
Mycobacterium tuberculosis
Macrófagos
topic Resistencia a Múltiples Medicamentos
Mycobacterium tuberculosis
Macrófagos
dc.description.none.fl_txt_mv Fil: Yokobori, Noemi. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Geffner, Laura. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Sabio y García, Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Schierloh, Pablo. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Barrera, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: de la Barrera, Silvia. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Sakai, Shunsuke. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Kawamura, Ikuo. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Mitsuyama, Masao. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Kyoto; Japón.
Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Sasiain, María Del Carmen. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
Mycobacterium tuberculosis has a considerable degree of genetic variability resulting in different epidemiology and disease outcomes. We evaluated the pathogen-host cell interaction of two genetically closely-related multidrug-resistant M. tuberculosis strains of the Haarlem family, namely the strain M, responsible for an extensive multidrug-resistant tuberculosis outbreak, and its kin strain 410 which caused a single case in two decades. Intracellular growth and cytokine responses were evaluated in human monocyte-derived macrophages and dU937 macrophage-like cells. In monocyte-derived macrophages, strain M grew more slowly and induced lower levels of TNF-α and IL-10 than 410, contrasting with previous studies with other strains, where a direct correlation was observed between increased intracellular growth and epidemiological success. On the other hand, in dU937 cells, no difference in growth was observed between both strains, and strain M induced significantly higher TNF-α levels than strain 410. We found that both cell models differed critically in the expression of receptors for M. tuberculosis entry, which might explain the different infection outcomes. Our results in monocyte-derived macrophages suggest that strain M relies on a modest replication rate and cytokine induction, keeping a state of quiescence and remaining rather unnoticed by the host. Collectively, our results underscore the impact of M. tuberculosis intra-species variations on the outcome of host cell infection and show that results can differ depending on the in vitro infection model.
description Fil: Yokobori, Noemi. Instituto de Medicina Experimental (IMEX) – CONICET, Academia Nacional de Medicina, Buenos Aires; Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1567-1348
https://www.sciencedirect.com/science/article/abs/pii/S1567134813000245?via%3Dihub
http://sgc.anlis.gob.ar/handle/123456789/2048
10.1016/j.meegid.2013.01.007
identifier_str_mv 1567-1348
10.1016/j.meegid.2013.01.007
url https://www.sciencedirect.com/science/article/abs/pii/S1567134813000245?via%3Dihub
http://sgc.anlis.gob.ar/handle/123456789/2048
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Infection, Genetics and Evolution 2013; 16:151-6.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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