Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies

Autores
Rojas, Florencia D; Córdoba, Susana; Sosa, María de los A; Zalazar, Laura C; Fernandez, Mariana; Cattana, María E; Alegre, Liliana R; Carrillo-Muñoz, Alfonso; Giusiano, Gustavo E
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Zalazar, Laura C. Universidad Nacional del Nordeste. Facultad de Humanidades. Cátedra de Matématica Aplicada; Chaco, Argentina.
Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Alegre, Liliana R. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Carrillo-Muñoz, Alfonso. ACIAM. Departamento de Microbiología; Barcelona, España.
Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro susceptibility of Malassezia yeast against antifungal agents using broth microdilution and disc diffusion methods, then to compare both methodologies. Fifty Malassezia isolates were studied. Microdilution method was performed as described in reference document and agar diffusion test was performed using antifungal tablets and discs. To support growth, culture media were supplemented. To correlate methods, linear regression analysis and categorical agreement was determined. The strongest linear association was observed for fluconazole and miconazole. The highest agreement between both methods was observed for itraconazole and voriconazole and the lowest for amphotericin B and fluconazole. Although modifications made to disc diffusion method allowed to obtain susceptibility data for Malassezia yeast, variables cannot be associated through a linear correlation model, indicating that inhibition zone values cannot predict MIC value. According to the results, disc diffusion assay may not represent an alternative to determine antifungal susceptibility of Malassezia yeast.
Materia
Malassezia
Antifúngicos
Anfotericina B
Pruebas de Sensibilidad Microbiana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1914
Acceder
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologiesRojas, Florencia DCórdoba, SusanaSosa, María de los AZalazar, Laura CFernandez, MarianaCattana, María EAlegre, Liliana RCarrillo-Muñoz, AlfonsoGiusiano, Gustavo EMalasseziaAntifúngicosAnfotericina BPruebas de Sensibilidad MicrobianaFil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Zalazar, Laura C. Universidad Nacional del Nordeste. Facultad de Humanidades. Cátedra de Matématica Aplicada; Chaco, Argentina.Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Alegre, Liliana R. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.Fil: Carrillo-Muñoz, Alfonso. ACIAM. Departamento de Microbiología; Barcelona, España.Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro susceptibility of Malassezia yeast against antifungal agents using broth microdilution and disc diffusion methods, then to compare both methodologies. Fifty Malassezia isolates were studied. Microdilution method was performed as described in reference document and agar diffusion test was performed using antifungal tablets and discs. To support growth, culture media were supplemented. To correlate methods, linear regression analysis and categorical agreement was determined. The strongest linear association was observed for fluconazole and miconazole. The highest agreement between both methods was observed for itraconazole and voriconazole and the lowest for amphotericin B and fluconazole. Although modifications made to disc diffusion method allowed to obtain susceptibility data for Malassezia yeast, variables cannot be associated through a linear correlation model, indicating that inhibition zone values cannot predict MIC value. According to the results, disc diffusion assay may not represent an alternative to determine antifungal susceptibility of Malassezia yeast.2017-02info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1439-0507http://sgc.anlis.gob.ar/handle/123456789/191410.1111/myc.12556Mycosesnoneinfo:eu-repo/semantics/openAccessengreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS2025-09-04T11:17:52Zoai:sgc.anlis.gob.ar:Publications/123456789/1914Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:17:52.42Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
title Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
spellingShingle Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
Rojas, Florencia D
Malassezia
Antifúngicos
Anfotericina B
Pruebas de Sensibilidad Microbiana
title_short Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
title_full Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
title_fullStr Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
title_full_unstemmed Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
title_sort Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
dc.creator.none.fl_str_mv Rojas, Florencia D
Córdoba, Susana
Sosa, María de los A
Zalazar, Laura C
Fernandez, Mariana
Cattana, María E
Alegre, Liliana R
Carrillo-Muñoz, Alfonso
Giusiano, Gustavo E
author Rojas, Florencia D
author_facet Rojas, Florencia D
Córdoba, Susana
Sosa, María de los A
Zalazar, Laura C
Fernandez, Mariana
Cattana, María E
Alegre, Liliana R
Carrillo-Muñoz, Alfonso
Giusiano, Gustavo E
author_role author
author2 Córdoba, Susana
Sosa, María de los A
Zalazar, Laura C
Fernandez, Mariana
Cattana, María E
Alegre, Liliana R
Carrillo-Muñoz, Alfonso
Giusiano, Gustavo E
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malassezia
Antifúngicos
Anfotericina B
Pruebas de Sensibilidad Microbiana
topic Malassezia
Antifúngicos
Anfotericina B
Pruebas de Sensibilidad Microbiana
dc.description.none.fl_txt_mv Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Zalazar, Laura C. Universidad Nacional del Nordeste. Facultad de Humanidades. Cátedra de Matématica Aplicada; Chaco, Argentina.
Fil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Alegre, Liliana R. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
Fil: Carrillo-Muñoz, Alfonso. ACIAM. Departamento de Microbiología; Barcelona, España.
Fil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro susceptibility of Malassezia yeast against antifungal agents using broth microdilution and disc diffusion methods, then to compare both methodologies. Fifty Malassezia isolates were studied. Microdilution method was performed as described in reference document and agar diffusion test was performed using antifungal tablets and discs. To support growth, culture media were supplemented. To correlate methods, linear regression analysis and categorical agreement was determined. The strongest linear association was observed for fluconazole and miconazole. The highest agreement between both methods was observed for itraconazole and voriconazole and the lowest for amphotericin B and fluconazole. Although modifications made to disc diffusion method allowed to obtain susceptibility data for Malassezia yeast, variables cannot be associated through a linear correlation model, indicating that inhibition zone values cannot predict MIC value. According to the results, disc diffusion assay may not represent an alternative to determine antifungal susceptibility of Malassezia yeast.
description Fil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1439-0507
http://sgc.anlis.gob.ar/handle/123456789/1914
10.1111/myc.12556
identifier_str_mv 1439-0507
10.1111/myc.12556
url http://sgc.anlis.gob.ar/handle/123456789/1914
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mycoses
dc.rights.none.fl_str_mv none
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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