PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA

Autores
Hong, Xiao-Kun; Zhang, Xuan; Fusco, Octavio Alejandro; Lan, You-Gen; Lun, Zhao-Rong; Lai, De-Hua
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Hong, Xiao-Kun. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Zhang, Xuan. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Fusco, Octavio Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Lan, You-Gen. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Lun, Zhao-Rong. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Lai, De-Hua. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. Its closely related species with global distribution, Trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. These trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. In this study, we have developed a rapid, sensitive and reliable PCR method for the diagnosis of T. lewisi and T. musculi. The T. lewisi-specific amplicons were not produced by other Trypanosoma, such as T. musculi, T. brucei complex or T. cruzi, neither by an outgroup of Leishmania amazonensis. The detection limits of the three pairs of T. lewisi-specific primers were 50ng, 1ng and 10ng of total DNA, respectively. The primers designed for T. musculi primers showed specifically that amplicon strictly in T. musculi and their detection limits were 10ng and 1ng of total DNA. To simplify the detection process, we managed to apply our method directly on tail blood samples without complicated DNA purification. In conclusion, PCR with our primers could be a highly sensitive, specific protocol to detect and distinguish T. lewisi and T. musculi from other trypanosomes.
Fuente
Acta Trópica 2017;171:207-212
Materia
ADN
Trypanosoma
Geomorfología
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1440
Acceder
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network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNAHong, Xiao-KunZhang, XuanFusco, Octavio AlejandroLan, You-GenLun, Zhao-RongLai, De-HuaADNTrypanosomaGeomorfologíaFil: Hong, Xiao-Kun. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.Fil: Zhang, Xuan. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.Fil: Fusco, Octavio Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Lan, You-Gen. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.Fil: Lun, Zhao-Rong. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.Fil: Lai, De-Hua. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.Trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. Its closely related species with global distribution, Trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. These trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. In this study, we have developed a rapid, sensitive and reliable PCR method for the diagnosis of T. lewisi and T. musculi. The T. lewisi-specific amplicons were not produced by other Trypanosoma, such as T. musculi, T. brucei complex or T. cruzi, neither by an outgroup of Leishmania amazonensis. The detection limits of the three pairs of T. lewisi-specific primers were 50ng, 1ng and 10ng of total DNA, respectively. The primers designed for T. musculi primers showed specifically that amplicon strictly in T. musculi and their detection limits were 10ng and 1ng of total DNA. To simplify the detection process, we managed to apply our method directly on tail blood samples without complicated DNA purification. In conclusion, PCR with our primers could be a highly sensitive, specific protocol to detect and distinguish T. lewisi and T. musculi from other trypanosomes.2017-07info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1873-6254http://sgc.anlis.gob.ar/handle/123456789/144010.1016/j.actatropica.2017.04.007Acta Trópica 2017;171:207-212reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISActa tropicanoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:20Zoai:sgc.anlis.gob.ar:Publications/123456789/1440Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:20.714Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
title PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
spellingShingle PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
Hong, Xiao-Kun
ADN
Trypanosoma
Geomorfología
title_short PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
title_full PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
title_fullStr PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
title_full_unstemmed PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
title_sort PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
dc.creator.none.fl_str_mv Hong, Xiao-Kun
Zhang, Xuan
Fusco, Octavio Alejandro
Lan, You-Gen
Lun, Zhao-Rong
Lai, De-Hua
author Hong, Xiao-Kun
author_facet Hong, Xiao-Kun
Zhang, Xuan
Fusco, Octavio Alejandro
Lan, You-Gen
Lun, Zhao-Rong
Lai, De-Hua
author_role author
author2 Zhang, Xuan
Fusco, Octavio Alejandro
Lan, You-Gen
Lun, Zhao-Rong
Lai, De-Hua
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ADN
Trypanosoma
Geomorfología
topic ADN
Trypanosoma
Geomorfología
dc.description.none.fl_txt_mv Fil: Hong, Xiao-Kun. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Zhang, Xuan. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Fusco, Octavio Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Lan, You-Gen. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Lun, Zhao-Rong. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Fil: Lai, De-Hua. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
Trypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. Its closely related species with global distribution, Trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. These trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. In this study, we have developed a rapid, sensitive and reliable PCR method for the diagnosis of T. lewisi and T. musculi. The T. lewisi-specific amplicons were not produced by other Trypanosoma, such as T. musculi, T. brucei complex or T. cruzi, neither by an outgroup of Leishmania amazonensis. The detection limits of the three pairs of T. lewisi-specific primers were 50ng, 1ng and 10ng of total DNA, respectively. The primers designed for T. musculi primers showed specifically that amplicon strictly in T. musculi and their detection limits were 10ng and 1ng of total DNA. To simplify the detection process, we managed to apply our method directly on tail blood samples without complicated DNA purification. In conclusion, PCR with our primers could be a highly sensitive, specific protocol to detect and distinguish T. lewisi and T. musculi from other trypanosomes.
description Fil: Hong, Xiao-Kun. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1873-6254
http://sgc.anlis.gob.ar/handle/123456789/1440
10.1016/j.actatropica.2017.04.007
identifier_str_mv 1873-6254
10.1016/j.actatropica.2017.04.007
url http://sgc.anlis.gob.ar/handle/123456789/1440
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta tropica
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Acta Trópica 2017;171:207-212
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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