Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples
- Autores
- Cura, Carolina Inés; Duffy, Tomas; Lucero, Raúl; Bisio, Margarita María Catalina; Péneau, Julie; Jimenez Coello, Matilde; Calabuig, Eva; Gimenez, María J.; Valencia Ayala, Edward; Kjos, Sonia A.; Santalla, José; Mahaney, Susan M.; Cayo, Nelly M.; Nagel, Claudia; Barcan, Laura; Málaga Machaca, Edith S.; Acosta Viana, Karla Y.; Brutus, Laurent; Ocampo, Susana B.; Aznar, Christine; Cuba Cuba, Cesar A.; Gurtler, Ricardo Esteban; Ramsey, Janine M.; Ribeiro, Isabela; Vandeberg, John I.; Yadon, Zaida E.; Osuna, Antonio; Schijman, Alejandro Gabriel
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background. Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI?TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we aimed to develop a multiplex Real-Time PCR method to identify the six T. cruzi DTUs using TaqMan probes (MTq-PCR).Methods/Principal Findings. The MTq-PCR has been evaluated in 39 cultured stocks and 307 biological samples from vectors, reservoirs and patients from different geographical regions and transmission cycles in comparison with a multi-locus conventional PCR algorithm. The MTq-PCR was inclusive for laboratory stocks and natural isolates and sensitive for direct typing of different biological samples from vectors, reservoirs and patients with acute, congenital infection or Chagas reactivation. The first round SL-IR MTq-PCR detected 1 fg DNA/reaction tube of TcI, TcII and TcIII and 1 pg DNA/reaction tube of TcIV, TcV and TcVI reference strains. The MTq-PCR was able to characterize DTUs in 83% of triatomine and 96% of reservoir samples that had been typed by conventional PCR methods. Regarding clinical samples, 100% of those derived from acute infected patients, 62.5% from congenitally infected children and 50% from patients with clinical reactivation could be genotyped. Sensitivity for direct typing of blood samples from chronic Chagas disease patients (32.8% from asymptomatic and 22.2% from symptomatic patients) and mixed infections was lower than that of the conventional PCR algorithm.Conclusions/Significance. Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.
Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina
Fil: Duffy, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina
Fil: Lucero, Raúl. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina
Fil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina
Fil: Péneau, Julie. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana Francesa
Fil: Jimenez Coello, Matilde. Universidad Autónoma de Yucatán; México
Fil: Calabuig, Eva. Hospital Politécnico LA FE; España
Fil: Gimenez, María J.. Hospital Politécnico LA FE; España
Fil: Valencia Ayala, Edward. Universidad Peruana Cayetano Heredia; Perú
Fil: Kjos, Sonia A.. University Of Minnesota; Estados Unidos
Fil: Santalla, José. Instituto Nacional de Laboratorios en Salud; Bolivia
Fil: Mahaney, Susan M.. Texas Biomedical Research Institute; Estados Unidos
Fil: Cayo, Nelly M.. Universidad Nacional de Jujuy; Argentina
Fil: Nagel, Claudia. Fundacion Favaloro; Argentina
Fil: Barcan, Laura. Hospital Italiano de Buenos Aires; Argentina
Fil: Málaga Machaca, Edith S.. Universidad Peruana Cayetano Heredia; Perú
Fil: Acosta Viana, Karla Y.. Universidad Autónoma de Yucatán; México
Fil: Brutus, Laurent. University Paris Descartes; Francia
Fil: Ocampo, Susana B.. Universidad Nacional de Jujuy; Argentina
Fil: Aznar, Christine. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana Francesa
Fil: Cuba Cuba, Cesar A.. Universidade Do Brasilia; Brasil
Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Ramsey, Janine M.. University Paris Descartes; México
Fil: Ribeiro, Isabela. Drugs and Neglected Diseases Initiative; Suiza
Fil: Vandeberg, John I.. Texas Biomedical Research Institute; Estados Unidos
Fil: Yadon, Zaida E.. Organizacion Mundial de la Salud; Argentina
Fil: Osuna, Antonio. Universidad de Granada; España
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina - Materia
-
Real Time PCR
Molecular genotyping
TaqMan
Trypanosoma cruzi - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/7918
Ver los metadatos del registro completo
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Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical SamplesCura, Carolina InésDuffy, TomasLucero, RaúlBisio, Margarita María CatalinaPéneau, JulieJimenez Coello, MatildeCalabuig, EvaGimenez, María J.Valencia Ayala, EdwardKjos, Sonia A.Santalla, JoséMahaney, Susan M.Cayo, Nelly M.Nagel, ClaudiaBarcan, LauraMálaga Machaca, Edith S.Acosta Viana, Karla Y.Brutus, LaurentOcampo, Susana B.Aznar, ChristineCuba Cuba, Cesar A.Gurtler, Ricardo EstebanRamsey, Janine M.Ribeiro, IsabelaVandeberg, John I.Yadon, Zaida E.Osuna, AntonioSchijman, Alejandro GabrielReal Time PCRMolecular genotypingTaqManTrypanosoma cruzihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background. Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI?TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we aimed to develop a multiplex Real-Time PCR method to identify the six T. cruzi DTUs using TaqMan probes (MTq-PCR).Methods/Principal Findings. The MTq-PCR has been evaluated in 39 cultured stocks and 307 biological samples from vectors, reservoirs and patients from different geographical regions and transmission cycles in comparison with a multi-locus conventional PCR algorithm. The MTq-PCR was inclusive for laboratory stocks and natural isolates and sensitive for direct typing of different biological samples from vectors, reservoirs and patients with acute, congenital infection or Chagas reactivation. The first round SL-IR MTq-PCR detected 1 fg DNA/reaction tube of TcI, TcII and TcIII and 1 pg DNA/reaction tube of TcIV, TcV and TcVI reference strains. The MTq-PCR was able to characterize DTUs in 83% of triatomine and 96% of reservoir samples that had been typed by conventional PCR methods. Regarding clinical samples, 100% of those derived from acute infected patients, 62.5% from congenitally infected children and 50% from patients with clinical reactivation could be genotyped. Sensitivity for direct typing of blood samples from chronic Chagas disease patients (32.8% from asymptomatic and 22.2% from symptomatic patients) and mixed infections was lower than that of the conventional PCR algorithm.Conclusions/Significance. Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; ArgentinaFil: Duffy, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; ArgentinaFil: Lucero, Raúl. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; ArgentinaFil: Péneau, Julie. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana FrancesaFil: Jimenez Coello, Matilde. Universidad Autónoma de Yucatán; MéxicoFil: Calabuig, Eva. Hospital Politécnico LA FE; EspañaFil: Gimenez, María J.. Hospital Politécnico LA FE; EspañaFil: Valencia Ayala, Edward. Universidad Peruana Cayetano Heredia; PerúFil: Kjos, Sonia A.. University Of Minnesota; Estados UnidosFil: Santalla, José. Instituto Nacional de Laboratorios en Salud; BoliviaFil: Mahaney, Susan M.. Texas Biomedical Research Institute; Estados UnidosFil: Cayo, Nelly M.. Universidad Nacional de Jujuy; ArgentinaFil: Nagel, Claudia. Fundacion Favaloro; ArgentinaFil: Barcan, Laura. Hospital Italiano de Buenos Aires; ArgentinaFil: Málaga Machaca, Edith S.. Universidad Peruana Cayetano Heredia; PerúFil: Acosta Viana, Karla Y.. Universidad Autónoma de Yucatán; MéxicoFil: Brutus, Laurent. University Paris Descartes; FranciaFil: Ocampo, Susana B.. Universidad Nacional de Jujuy; ArgentinaFil: Aznar, Christine. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana FrancesaFil: Cuba Cuba, Cesar A.. Universidade Do Brasilia; BrasilFil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Ramsey, Janine M.. University Paris Descartes; MéxicoFil: Ribeiro, Isabela. Drugs and Neglected Diseases Initiative; SuizaFil: Vandeberg, John I.. Texas Biomedical Research Institute; Estados UnidosFil: Yadon, Zaida E.. Organizacion Mundial de la Salud; ArgentinaFil: Osuna, Antonio. Universidad de Granada; EspañaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; ArgentinaPublic Library Of Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7918Cura, Carolina Inés; Duffy, Tomas; Lucero, Raúl; Bisio, Margarita María Catalina; Péneau, Julie; et al.; Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples; Public Library Of Science; Neglected Tropical Diseases; 9; 5; 5-2015; 3765-37651935-2735enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003765info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437652/info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003765info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:50Zoai:ri.conicet.gov.ar:11336/7918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:50.584CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| title |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| spellingShingle |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples Cura, Carolina Inés Real Time PCR Molecular genotyping TaqMan Trypanosoma cruzi |
| title_short |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| title_full |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| title_fullStr |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| title_full_unstemmed |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| title_sort |
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples |
| dc.creator.none.fl_str_mv |
Cura, Carolina Inés Duffy, Tomas Lucero, Raúl Bisio, Margarita María Catalina Péneau, Julie Jimenez Coello, Matilde Calabuig, Eva Gimenez, María J. Valencia Ayala, Edward Kjos, Sonia A. Santalla, José Mahaney, Susan M. Cayo, Nelly M. Nagel, Claudia Barcan, Laura Málaga Machaca, Edith S. Acosta Viana, Karla Y. Brutus, Laurent Ocampo, Susana B. Aznar, Christine Cuba Cuba, Cesar A. Gurtler, Ricardo Esteban Ramsey, Janine M. Ribeiro, Isabela Vandeberg, John I. Yadon, Zaida E. Osuna, Antonio Schijman, Alejandro Gabriel |
| author |
Cura, Carolina Inés |
| author_facet |
Cura, Carolina Inés Duffy, Tomas Lucero, Raúl Bisio, Margarita María Catalina Péneau, Julie Jimenez Coello, Matilde Calabuig, Eva Gimenez, María J. Valencia Ayala, Edward Kjos, Sonia A. Santalla, José Mahaney, Susan M. Cayo, Nelly M. Nagel, Claudia Barcan, Laura Málaga Machaca, Edith S. Acosta Viana, Karla Y. Brutus, Laurent Ocampo, Susana B. Aznar, Christine Cuba Cuba, Cesar A. Gurtler, Ricardo Esteban Ramsey, Janine M. Ribeiro, Isabela Vandeberg, John I. Yadon, Zaida E. Osuna, Antonio Schijman, Alejandro Gabriel |
| author_role |
author |
| author2 |
Duffy, Tomas Lucero, Raúl Bisio, Margarita María Catalina Péneau, Julie Jimenez Coello, Matilde Calabuig, Eva Gimenez, María J. Valencia Ayala, Edward Kjos, Sonia A. Santalla, José Mahaney, Susan M. Cayo, Nelly M. Nagel, Claudia Barcan, Laura Málaga Machaca, Edith S. Acosta Viana, Karla Y. Brutus, Laurent Ocampo, Susana B. Aznar, Christine Cuba Cuba, Cesar A. Gurtler, Ricardo Esteban Ramsey, Janine M. Ribeiro, Isabela Vandeberg, John I. Yadon, Zaida E. Osuna, Antonio Schijman, Alejandro Gabriel |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Real Time PCR Molecular genotyping TaqMan Trypanosoma cruzi |
| topic |
Real Time PCR Molecular genotyping TaqMan Trypanosoma cruzi |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Background. Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI?TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we aimed to develop a multiplex Real-Time PCR method to identify the six T. cruzi DTUs using TaqMan probes (MTq-PCR).Methods/Principal Findings. The MTq-PCR has been evaluated in 39 cultured stocks and 307 biological samples from vectors, reservoirs and patients from different geographical regions and transmission cycles in comparison with a multi-locus conventional PCR algorithm. The MTq-PCR was inclusive for laboratory stocks and natural isolates and sensitive for direct typing of different biological samples from vectors, reservoirs and patients with acute, congenital infection or Chagas reactivation. The first round SL-IR MTq-PCR detected 1 fg DNA/reaction tube of TcI, TcII and TcIII and 1 pg DNA/reaction tube of TcIV, TcV and TcVI reference strains. The MTq-PCR was able to characterize DTUs in 83% of triatomine and 96% of reservoir samples that had been typed by conventional PCR methods. Regarding clinical samples, 100% of those derived from acute infected patients, 62.5% from congenitally infected children and 50% from patients with clinical reactivation could be genotyped. Sensitivity for direct typing of blood samples from chronic Chagas disease patients (32.8% from asymptomatic and 22.2% from symptomatic patients) and mixed infections was lower than that of the conventional PCR algorithm.Conclusions/Significance. Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production. Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina Fil: Duffy, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina Fil: Lucero, Raúl. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina Fil: Péneau, Julie. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana Francesa Fil: Jimenez Coello, Matilde. Universidad Autónoma de Yucatán; México Fil: Calabuig, Eva. Hospital Politécnico LA FE; España Fil: Gimenez, María J.. Hospital Politécnico LA FE; España Fil: Valencia Ayala, Edward. Universidad Peruana Cayetano Heredia; Perú Fil: Kjos, Sonia A.. University Of Minnesota; Estados Unidos Fil: Santalla, José. Instituto Nacional de Laboratorios en Salud; Bolivia Fil: Mahaney, Susan M.. Texas Biomedical Research Institute; Estados Unidos Fil: Cayo, Nelly M.. Universidad Nacional de Jujuy; Argentina Fil: Nagel, Claudia. Fundacion Favaloro; Argentina Fil: Barcan, Laura. Hospital Italiano de Buenos Aires; Argentina Fil: Málaga Machaca, Edith S.. Universidad Peruana Cayetano Heredia; Perú Fil: Acosta Viana, Karla Y.. Universidad Autónoma de Yucatán; México Fil: Brutus, Laurent. University Paris Descartes; Francia Fil: Ocampo, Susana B.. Universidad Nacional de Jujuy; Argentina Fil: Aznar, Christine. Laboratoire Hospitalier et Universitaire-CH Andrée Rosemon; Guayana Francesa Fil: Cuba Cuba, Cesar A.. Universidade Do Brasilia; Brasil Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Ramsey, Janine M.. University Paris Descartes; México Fil: Ribeiro, Isabela. Drugs and Neglected Diseases Initiative; Suiza Fil: Vandeberg, John I.. Texas Biomedical Research Institute; Estados Unidos Fil: Yadon, Zaida E.. Organizacion Mundial de la Salud; Argentina Fil: Osuna, Antonio. Universidad de Granada; España Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones En Ingeniería Genética y Biología Molecular; Argentina |
| description |
Background. Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI?TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we aimed to develop a multiplex Real-Time PCR method to identify the six T. cruzi DTUs using TaqMan probes (MTq-PCR).Methods/Principal Findings. The MTq-PCR has been evaluated in 39 cultured stocks and 307 biological samples from vectors, reservoirs and patients from different geographical regions and transmission cycles in comparison with a multi-locus conventional PCR algorithm. The MTq-PCR was inclusive for laboratory stocks and natural isolates and sensitive for direct typing of different biological samples from vectors, reservoirs and patients with acute, congenital infection or Chagas reactivation. The first round SL-IR MTq-PCR detected 1 fg DNA/reaction tube of TcI, TcII and TcIII and 1 pg DNA/reaction tube of TcIV, TcV and TcVI reference strains. The MTq-PCR was able to characterize DTUs in 83% of triatomine and 96% of reservoir samples that had been typed by conventional PCR methods. Regarding clinical samples, 100% of those derived from acute infected patients, 62.5% from congenitally infected children and 50% from patients with clinical reactivation could be genotyped. Sensitivity for direct typing of blood samples from chronic Chagas disease patients (32.8% from asymptomatic and 22.2% from symptomatic patients) and mixed infections was lower than that of the conventional PCR algorithm.Conclusions/Significance. Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-05 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/7918 Cura, Carolina Inés; Duffy, Tomas; Lucero, Raúl; Bisio, Margarita María Catalina; Péneau, Julie; et al.; Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples; Public Library Of Science; Neglected Tropical Diseases; 9; 5; 5-2015; 3765-3765 1935-2735 |
| url |
http://hdl.handle.net/11336/7918 |
| identifier_str_mv |
Cura, Carolina Inés; Duffy, Tomas; Lucero, Raúl; Bisio, Margarita María Catalina; Péneau, Julie; et al.; Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples; Public Library Of Science; Neglected Tropical Diseases; 9; 5; 5-2015; 3765-3765 1935-2735 |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003765 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4437652/ info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003765 |
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