Three-year assessment of methicillin-resistant Staphylococcus aureus clones in Latin America from 1996 to 1998

• Autores: De Sousa, Marta Aires; Miragaia, María; Sanches, Ilda Santos; Ávila, Sofía; Adamson, Inger; Casagrande, Silvana T.; Brandileone, Maria Cristina; Palacio, Rosario; Dell'Acqua, Lillia; Hortal, María; Camou, Teresa; Rossi, Alicia; Velazquez-Meza, Maria Elena; Echániz-Avilés, Gabriela; Solorzano-Santos, Fortino; Heitmann, Ingrid; De Lencastre, Hermínia
• Año de publicación: 2001
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of Clinical Microbiology, 2001; 39(6): 2197-2205.
• Materia: Staphylococcus aureus; Staphylococcus aureus Resistente a Meticilina; América Latina
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: De Sousa, Marta Aires. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Miragaia, Maria. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Sanches, Ilda Santos. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Ávila, Sofia. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Adamson, Inger. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Casagrande, Silvana T. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Brandileone, Maria Crisitna. Instituto Adolfo Lutz; Brazil.
Fil: Palacio, Rosario. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Dell'Acqua, Lillia. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Hortal, Maria. Ministerio de Salud. Laboratorio de Salud Pública; Uruguay.
Fil: Camou, Teresa. Ministerio de Salud. Laboratorio de Salud Pública; Uruguay.
Fil: Rossi, Alicia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Velazquez-Meza, Maria Elena. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Fil: Echániz-Avilés, Gabriela. Instituto Nacional de Salud Pública. Centro de Investigaciones sobre Enfermedades Infecciosas; México.
Fil: Solorzano-Santos, Fortino. Hospital de Pediatria; México.
Fil: Heitmann, Ingrid. Instituto de Salud Pública de Chile; Chile.
Fil: De Lencastre, Hermínia. Universidade Nova de Lisboa. Molecular Genetics Laboratory; Portugal.
Four hundred ninety-nine methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from 1996 to 1998 from 22 hospitals in five countries of Latin America—Argentina, Brazil, Chile, Uruguay and Mexico— were examined for antimicrobial susceptibility and clonal type in order to define the endemic clones in those hospitals. The hybridization of ClaI restriction digests with the mecA- and Tn554-specific DNA probes combined with pulsed-field gel electrophoresis of chromosomal SmaI digests (ClaI-mecA::ClaI-Tn554::PFGE clonal types) documented not only the predominance and persistence of the Brazilian clone (XI::B::B) in Brazil (97%) and Argentina (86%) but also its massive dissemination to Uruguay (100%). Moreover, a close relative of the Brazilian clone (XI::k::B) was highly represented in Chile (53%) together with a novel clone (47%) (II::E*::F) resistant to pencillin, oxacillin, ciprofloxacin, chloramphenicol, clindamycin, erythromycin, and gentamicin. A unique clonal type (I::NH::M) was detected in Mexico among pediatric isolates and was resistant to penicillin, oxacillin, and gentamicin only. This study clearly documented the very large capacity for geographic expansion and the persistence of the Brazilian clone, contributing not only to the increasing uniformity of the MRSA in South America but worldwide as well.