Study on the obtaining of Tityus trivittatus venom in Argentina

Autores
de Roodt, Adolfo R.; Lanari, Laura Cecilia; Laskowicz, Rodrigo Daniel; Costa de Oliveira, Vanessa; Litwin, Silvana; Calderon, Leandro; Damin, Carlos Fabián; Dokmetjian, Christian; Dolab, Jorge A.; Lago, Néstor Rubén; Lértora, Emiliano
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: de Roodt, Adolfo Rafael. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Lanari, Laura Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Laskowicz, Rodrigo Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Costa de Oliveira, Vanessa. Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina; Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.
Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Calderon, Leandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Damin, Carlos Fabián. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Lago, Néstor Rubén. Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.
Fil: Lértora, Emiliano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Envenomation by scorpions of the genus Tityus is an important public health problem in Argentina, involving near 8000 stings and 2 deaths each year. Treatment for envenomation is the use of specific antivenom and intensive hospital care. Antivenom is produced by the Ministry of Health and freely distributed throughout the country. For antivenom production it is necessary to collect scorpion venom, which is a difficult task because although scorpions can be found in Argentina, they are less abundant than in warmer latitudes. For this reason venom collection constitutes a bottleneck for antivenom production. Although in Argentina several species of Tityus can be found, most of the accidents are caused by Tityus trivittatus, and the venom of this scorpion has historically been the venom used for antivenom production. We analyzed retrospectively 26 pools of telson homogenates (6964 telsons) and 37 pools of milked venom obtained by electrical stimulation (equivalent to 6841 milkings). Lethal potencies of samples from different provinces were very similar, although venom from scorpions of Buenos Aires city showed the lowest potency. The venom obtained by milking (median LD50 12.3 μg), provided batches containing LD50s more potent when compared with the venom obtained from telson homogenates (p < 0.0001). Many batches of telson homogenates (30%) showed lower potencies than acceptable for antivenom production and control. In addition to the study of the venom yield, the records of immunization of horses, the potency of the batches and the protein content of each batch of anti-scorpion antivenom produced were analyzed, comparing those produced using milked venom with those using telson homogenates as immunogens. Batches produced using milked venom required a shorter period of immunization (p < 0.0001), rendered higher neutralizing titers (p 0.0350) and possessed lower protein content (p 0.0092). Results clearly showed that the milking of scorpions is a more efficient tool to obtain venom for antivenom production in comparison to the use of telson homogenates.
Fuente
Toxicon 2019; 159:5-13.
Materia
Venenos
Antivenenos
Toxicidad
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1359

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oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/1359
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Study on the obtaining of Tityus trivittatus venom in Argentinade Roodt, Adolfo R.Lanari, Laura CeciliaLaskowicz, Rodrigo DanielCosta de Oliveira, VanessaLitwin, SilvanaCalderon, LeandroDamin, Carlos FabiánDokmetjian, ChristianDolab, Jorge A.Lago, Néstor RubénLértora, EmilianoVenenosAntivenenosToxicidadFil: de Roodt, Adolfo Rafael. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.Fil: Lanari, Laura Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.Fil: Laskowicz, Rodrigo Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.Fil: Costa de Oliveira, Vanessa. Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina; Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Calderon, Leandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Damin, Carlos Fabián. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Lago, Néstor Rubén. Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.Fil: Lértora, Emiliano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Envenomation by scorpions of the genus Tityus is an important public health problem in Argentina, involving near 8000 stings and 2 deaths each year. Treatment for envenomation is the use of specific antivenom and intensive hospital care. Antivenom is produced by the Ministry of Health and freely distributed throughout the country. For antivenom production it is necessary to collect scorpion venom, which is a difficult task because although scorpions can be found in Argentina, they are less abundant than in warmer latitudes. For this reason venom collection constitutes a bottleneck for antivenom production. Although in Argentina several species of Tityus can be found, most of the accidents are caused by Tityus trivittatus, and the venom of this scorpion has historically been the venom used for antivenom production. We analyzed retrospectively 26 pools of telson homogenates (6964 telsons) and 37 pools of milked venom obtained by electrical stimulation (equivalent to 6841 milkings). Lethal potencies of samples from different provinces were very similar, although venom from scorpions of Buenos Aires city showed the lowest potency. The venom obtained by milking (median LD50 12.3 μg), provided batches containing LD50s more potent when compared with the venom obtained from telson homogenates (p < 0.0001). Many batches of telson homogenates (30%) showed lower potencies than acceptable for antivenom production and control. In addition to the study of the venom yield, the records of immunization of horses, the potency of the batches and the protein content of each batch of anti-scorpion antivenom produced were analyzed, comparing those produced using milked venom with those using telson homogenates as immunogens. Batches produced using milked venom required a shorter period of immunization (p < 0.0001), rendered higher neutralizing titers (p 0.0350) and possessed lower protein content (p 0.0092). Results clearly showed that the milking of scorpions is a more efficient tool to obtain venom for antivenom production in comparison to the use of telson homogenates.Elsevier2019-03-01info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0041-0101http://sgc.anlis.gob.ar/handle/123456789/135910.1016/j.toxicon.2018.12.004Toxicon 2019; 159:5-13.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISToxicon : official journal of the International Society on Toxinologynoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:18Zoai:sgc.anlis.gob.ar:Publications/123456789/1359Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:18.777Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Study on the obtaining of Tityus trivittatus venom in Argentina
title Study on the obtaining of Tityus trivittatus venom in Argentina
spellingShingle Study on the obtaining of Tityus trivittatus venom in Argentina
de Roodt, Adolfo R.
Venenos
Antivenenos
Toxicidad
title_short Study on the obtaining of Tityus trivittatus venom in Argentina
title_full Study on the obtaining of Tityus trivittatus venom in Argentina
title_fullStr Study on the obtaining of Tityus trivittatus venom in Argentina
title_full_unstemmed Study on the obtaining of Tityus trivittatus venom in Argentina
title_sort Study on the obtaining of Tityus trivittatus venom in Argentina
dc.creator.none.fl_str_mv de Roodt, Adolfo R.
Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Costa de Oliveira, Vanessa
Litwin, Silvana
Calderon, Leandro
Damin, Carlos Fabián
Dokmetjian, Christian
Dolab, Jorge A.
Lago, Néstor Rubén
Lértora, Emiliano
author de Roodt, Adolfo R.
author_facet de Roodt, Adolfo R.
Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Costa de Oliveira, Vanessa
Litwin, Silvana
Calderon, Leandro
Damin, Carlos Fabián
Dokmetjian, Christian
Dolab, Jorge A.
Lago, Néstor Rubén
Lértora, Emiliano
author_role author
author2 Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Costa de Oliveira, Vanessa
Litwin, Silvana
Calderon, Leandro
Damin, Carlos Fabián
Dokmetjian, Christian
Dolab, Jorge A.
Lago, Néstor Rubén
Lértora, Emiliano
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Venenos
Antivenenos
Toxicidad
topic Venenos
Antivenenos
Toxicidad
dc.description.none.fl_txt_mv Fil: de Roodt, Adolfo Rafael. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Lanari, Laura Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Laskowicz, Rodrigo Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Costa de Oliveira, Vanessa. Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina; Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.
Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Calderon, Leandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Damin, Carlos Fabián. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Fil: Lago, Néstor Rubén. Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires; Argentina.
Fil: Lértora, Emiliano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
Envenomation by scorpions of the genus Tityus is an important public health problem in Argentina, involving near 8000 stings and 2 deaths each year. Treatment for envenomation is the use of specific antivenom and intensive hospital care. Antivenom is produced by the Ministry of Health and freely distributed throughout the country. For antivenom production it is necessary to collect scorpion venom, which is a difficult task because although scorpions can be found in Argentina, they are less abundant than in warmer latitudes. For this reason venom collection constitutes a bottleneck for antivenom production. Although in Argentina several species of Tityus can be found, most of the accidents are caused by Tityus trivittatus, and the venom of this scorpion has historically been the venom used for antivenom production. We analyzed retrospectively 26 pools of telson homogenates (6964 telsons) and 37 pools of milked venom obtained by electrical stimulation (equivalent to 6841 milkings). Lethal potencies of samples from different provinces were very similar, although venom from scorpions of Buenos Aires city showed the lowest potency. The venom obtained by milking (median LD50 12.3 μg), provided batches containing LD50s more potent when compared with the venom obtained from telson homogenates (p < 0.0001). Many batches of telson homogenates (30%) showed lower potencies than acceptable for antivenom production and control. In addition to the study of the venom yield, the records of immunization of horses, the potency of the batches and the protein content of each batch of anti-scorpion antivenom produced were analyzed, comparing those produced using milked venom with those using telson homogenates as immunogens. Batches produced using milked venom required a shorter period of immunization (p < 0.0001), rendered higher neutralizing titers (p 0.0350) and possessed lower protein content (p 0.0092). Results clearly showed that the milking of scorpions is a more efficient tool to obtain venom for antivenom production in comparison to the use of telson homogenates.
description Fil: de Roodt, Adolfo Rafael. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-01
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0041-0101
http://sgc.anlis.gob.ar/handle/123456789/1359
10.1016/j.toxicon.2018.12.004
identifier_str_mv 0041-0101
10.1016/j.toxicon.2018.12.004
url http://sgc.anlis.gob.ar/handle/123456789/1359
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon : official journal of the International Society on Toxinology
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Toxicon 2019; 159:5-13.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
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