Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites

Autores
Tran, Tung; Andrés, Patricia; Petroni, Alejandro; Soler Bistue, Alfonso J. C.; Albornoz, Ezequiel Pablo; Zorreguieta, Angeles; Reyes Lamothe, Rodrigo; Sherratt, David J.; Corso, Alejandra; Tolmasky, Marcelo E.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
Fil: Tran, Tung. University of Oxford; Reino Unido. California State University Fullerton; Estados Unidos
Fil: Andrés, Patricia. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Soler Bistue, Alfonso Jc. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Albornoz, Ezequiel Pablo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Reyes Lamothe, Rodrigo. University of Oxford; Reino Unido
Fil: Sherratt, David J.. University of Oxford; Reino Unido
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Tolmasky, Marcelo E.. University of Oxford; Reino Unido. California State University Fullerton; Estados Unidos
Materia
Recombinación
Resistencia
Plasmido
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18346
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18346
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sitesTran, TungAndrés, PatriciaPetroni, AlejandroSoler Bistue, Alfonso J. C.Albornoz, Ezequiel PabloZorreguieta, AngelesReyes Lamothe, RodrigoSherratt, David J.Corso, AlejandraTolmasky, Marcelo E.RecombinaciónResistenciaPlasmidohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.Fil: Tran, Tung. University of Oxford; Reino Unido. California State University Fullerton; Estados UnidosFil: Andrés, Patricia. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Soler Bistue, Alfonso Jc. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Albornoz, Ezequiel Pablo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Reyes Lamothe, Rodrigo. University of Oxford; Reino UnidoFil: Sherratt, David J.. University of Oxford; Reino UnidoFil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Tolmasky, Marcelo E.. University of Oxford; Reino Unido. California State University Fullerton; Estados UnidosAmerican Society For Microbiology2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18346Tran, Tung; Andrés, Patricia; Petroni, Alejandro; Soler Bistue, Alfonso J. C.; Albornoz, Ezequiel Pablo; et al.; Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 56; 4; 1-2012; 1821-18270066-48041098-6596CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/56/4/1821.longinfo:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.06036-11info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:22Zoai:ri.conicet.gov.ar:11336/18346instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:22.428CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
title Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
spellingShingle Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
Tran, Tung
Recombinación
Resistencia
Plasmido
title_short Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
title_full Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
title_fullStr Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
title_full_unstemmed Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
title_sort Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites
dc.creator.none.fl_str_mv Tran, Tung
Andrés, Patricia
Petroni, Alejandro
Soler Bistue, Alfonso J. C.
Albornoz, Ezequiel Pablo
Zorreguieta, Angeles
Reyes Lamothe, Rodrigo
Sherratt, David J.
Corso, Alejandra
Tolmasky, Marcelo E.
author Tran, Tung
author_facet Tran, Tung
Andrés, Patricia
Petroni, Alejandro
Soler Bistue, Alfonso J. C.
Albornoz, Ezequiel Pablo
Zorreguieta, Angeles
Reyes Lamothe, Rodrigo
Sherratt, David J.
Corso, Alejandra
Tolmasky, Marcelo E.
author_role author
author2 Andrés, Patricia
Petroni, Alejandro
Soler Bistue, Alfonso J. C.
Albornoz, Ezequiel Pablo
Zorreguieta, Angeles
Reyes Lamothe, Rodrigo
Sherratt, David J.
Corso, Alejandra
Tolmasky, Marcelo E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Recombinación
Resistencia
Plasmido
topic Recombinación
Resistencia
Plasmido
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
Fil: Tran, Tung. University of Oxford; Reino Unido. California State University Fullerton; Estados Unidos
Fil: Andrés, Patricia. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Soler Bistue, Alfonso Jc. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Albornoz, Ezequiel Pablo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Reyes Lamothe, Rodrigo. University of Oxford; Reino Unido
Fil: Sherratt, David J.. University of Oxford; Reino Unido
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Tolmasky, Marcelo E.. University of Oxford; Reino Unido. California State University Fullerton; Estados Unidos
description Plasmids pPAB19-1, pPAB19-2, pPAB19-3, and pPAB19-4, isolated from Salmonella and Escherichia coli clinical strains from hospitals in Argentina, were completely sequenced. These plasmids include the qnrB19 gene and are 2,699, 3,082, 2,989, and 2,702 nucleotides long, respectively, and they share extensive homology among themselves and with other previously described small qnrB19-harboring plasmids. The genetic environment of qnrB19 in all four plasmids is identical to that in these other plasmids and in transposons such as Tn2012, Tn5387, and Tn5387-like. Nucleotide sequence comparisons among these and previously described plasmids showed a variable region characterized by being flanked by an oriT locus and a Xer recombination site. We propose that this arrangement could play a role in the evolution of plasmids and present a model for DNA swapping between plasmid molecules mediated by site-specific recombination events at oriT and a Xer target site.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18346
Tran, Tung; Andrés, Patricia; Petroni, Alejandro; Soler Bistue, Alfonso J. C.; Albornoz, Ezequiel Pablo; et al.; Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 56; 4; 1-2012; 1821-1827
0066-4804
1098-6596
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18346
identifier_str_mv Tran, Tung; Andrés, Patricia; Petroni, Alejandro; Soler Bistue, Alfonso J. C.; Albornoz, Ezequiel Pablo; et al.; Model for Plasmid Evolution Mediated by site-specific recombination at oriT and Xer sites; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 56; 4; 1-2012; 1821-1827
0066-4804
1098-6596
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/56/4/1821.long
info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.06036-11
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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