Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
- Autores
- Frungieri, Mónica B.; Gonzalez Calvar, Silvia I.; Parborell, Fernanda; Albrecht, Martin; Mayerhofer, Artur; Calandra, Ricardo Saúl
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.
Instituto Multidisciplinario de Biología Celular - Materia
-
Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83449
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Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone productionFrungieri, Mónica B.Gonzalez Calvar, Silvia I.Parborell, FernandaAlbrecht, MartinMayerhofer, ArturCalandra, Ricardo SaúlBioquímicaBiologíaCyclooxygenase-2Prostaglandin F2Leydig cellsWe have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.Instituto Multidisciplinario de Biología Celular2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf4476-4485http://sedici.unlp.edu.ar/handle/10915/83449enginfo:eu-repo/semantics/altIdentifier/issn/0013-7227info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2006-0090info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:01Zoai:sedici.unlp.edu.ar:10915/83449Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:01.871SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
title |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
spellingShingle |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production Frungieri, Mónica B. Bioquímica Biología Cyclooxygenase-2 Prostaglandin F2 Leydig cells |
title_short |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
title_full |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
title_fullStr |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
title_full_unstemmed |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
title_sort |
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production |
dc.creator.none.fl_str_mv |
Frungieri, Mónica B. Gonzalez Calvar, Silvia I. Parborell, Fernanda Albrecht, Martin Mayerhofer, Artur Calandra, Ricardo Saúl |
author |
Frungieri, Mónica B. |
author_facet |
Frungieri, Mónica B. Gonzalez Calvar, Silvia I. Parborell, Fernanda Albrecht, Martin Mayerhofer, Artur Calandra, Ricardo Saúl |
author_role |
author |
author2 |
Gonzalez Calvar, Silvia I. Parborell, Fernanda Albrecht, Martin Mayerhofer, Artur Calandra, Ricardo Saúl |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Bioquímica Biología Cyclooxygenase-2 Prostaglandin F2 Leydig cells |
topic |
Bioquímica Biología Cyclooxygenase-2 Prostaglandin F2 Leydig cells |
dc.description.none.fl_txt_mv |
We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes. Instituto Multidisciplinario de Biología Celular |
description |
We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-06-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/83449 |
url |
http://sedici.unlp.edu.ar/handle/10915/83449 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0013-7227 info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2006-0090 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 4476-4485 |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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