Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production

Autores
Frungieri, Mónica B.; Gonzalez Calvar, Silvia I.; Parborell, Fernanda; Albrecht, Martin; Mayerhofer, Artur; Calandra, Ricardo Saúl
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.
Instituto Multidisciplinario de Biología Celular
Materia
Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83449

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83449
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone productionFrungieri, Mónica B.Gonzalez Calvar, Silvia I.Parborell, FernandaAlbrecht, MartinMayerhofer, ArturCalandra, Ricardo SaúlBioquímicaBiologíaCyclooxygenase-2Prostaglandin F2Leydig cellsWe have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.Instituto Multidisciplinario de Biología Celular2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf4476-4485http://sedici.unlp.edu.ar/handle/10915/83449enginfo:eu-repo/semantics/altIdentifier/issn/0013-7227info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2006-0090info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:01Zoai:sedici.unlp.edu.ar:10915/83449Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:01.871SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
spellingShingle Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
Frungieri, Mónica B.
Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
title_short Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_full Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_fullStr Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_full_unstemmed Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_sort Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
dc.creator.none.fl_str_mv Frungieri, Mónica B.
Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
author Frungieri, Mónica B.
author_facet Frungieri, Mónica B.
Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
author_role author
author2 Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
topic Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
dc.description.none.fl_txt_mv We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.
Instituto Multidisciplinario de Biología Celular
description We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83449
url http://sedici.unlp.edu.ar/handle/10915/83449
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0013-7227
info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2006-0090
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
4476-4485
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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