Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake

Autores
Matzkin, Maria Eugenia; Pellizari, Eliana Herminia; Rossi, Soledad Paola; Calandra, Ricardo Saul; Cigorraga, Selva Beatriz; Frungieri, Monica Beatriz
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously described a stimulatory effect of testosterone on cyclooxygenase 2 (COX2) expression and prostaglandin (PG) synthesis, and the involvement of PGs in the modulation of testosterone production in Leydig cells of the seasonal breeder Syrian hamster. In this study, we investigated the existence of a COX2/PGs system in hamster Sertoli cells, its regulation by testosterone and FSH, and its effect on glucose uptake. COX2 expression was observed in Sertoli cells of both reproductively active and inactive adult hamsters. Testosterone and the plasma membrane-impermeable testosterone-BSA significantly induced COX2 expression, mitogen activated protein kinases 1/2 (MAPK1/2) phosphorylation and 15d-delta12,14PGJ2 production in Sertoli cells purified from photoperiodically regressed hamsters. These actions were abolished by the antiandrogen bicalutamide and by the inhibitor of MAPK kinase (MEK1/2) U0126, suggesting that testosterone exerts its stimulatory effect on COX2/PGs through a non-classical mechanism that involves the presence of androgen receptors and MAPK1/2 activation. FSH also stimulated COX2/PGs via MAPK1/2 phosphorylation. FSH and testosterone stimulate, whereas 15d-delta12,14PGJ2 via PPAR?× inhibits, [2,6-3H]-2-deoxy-D-glucose ([3H]-2-DOG) uptake. Meloxicam, a selective COX2 inhibitor, further increases [3H]-2-DOG uptake in the presence of FSH or testosterone. Thus, in addition to their positive effect, FSH and testosterone may also exert an indirect negative regulation on glucose uptake which involves the COX2/15d-delta12,14PGJ2/PPAR?× system. Overall, these results demonstrate the presence of a COX2/PG system in hamster Sertoli cells which might act as a local modulator of FSH and testosterone actions.
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pellizari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Rossi, Soledad Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Cyclooxygenase 2
Testosterone
Fsh
Sertoli Cells
Hamsters
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8470
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8470
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptakeMatzkin, Maria EugeniaPellizari, Eliana HerminiaRossi, Soledad PaolaCalandra, Ricardo SaulCigorraga, Selva BeatrizFrungieri, Monica BeatrizCyclooxygenase 2TestosteroneFshSertoli CellsHamstershttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We have previously described a stimulatory effect of testosterone on cyclooxygenase 2 (COX2) expression and prostaglandin (PG) synthesis, and the involvement of PGs in the modulation of testosterone production in Leydig cells of the seasonal breeder Syrian hamster. In this study, we investigated the existence of a COX2/PGs system in hamster Sertoli cells, its regulation by testosterone and FSH, and its effect on glucose uptake. COX2 expression was observed in Sertoli cells of both reproductively active and inactive adult hamsters. Testosterone and the plasma membrane-impermeable testosterone-BSA significantly induced COX2 expression, mitogen activated protein kinases 1/2 (MAPK1/2) phosphorylation and 15d-delta12,14PGJ2 production in Sertoli cells purified from photoperiodically regressed hamsters. These actions were abolished by the antiandrogen bicalutamide and by the inhibitor of MAPK kinase (MEK1/2) U0126, suggesting that testosterone exerts its stimulatory effect on COX2/PGs through a non-classical mechanism that involves the presence of androgen receptors and MAPK1/2 activation. FSH also stimulated COX2/PGs via MAPK1/2 phosphorylation. FSH and testosterone stimulate, whereas 15d-delta12,14PGJ2 via PPAR?× inhibits, [2,6-3H]-2-deoxy-D-glucose ([3H]-2-DOG) uptake. Meloxicam, a selective COX2 inhibitor, further increases [3H]-2-DOG uptake in the presence of FSH or testosterone. Thus, in addition to their positive effect, FSH and testosterone may also exert an indirect negative regulation on glucose uptake which involves the COX2/15d-delta12,14PGJ2/PPAR?× system. Overall, these results demonstrate the presence of a COX2/PG system in hamster Sertoli cells which might act as a local modulator of FSH and testosterone actions.Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Pellizari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Rossi, Soledad Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaElsevier2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8470Matzkin, Maria Eugenia; Pellizari, Eliana Herminia; Rossi, Soledad Paola; Calandra, Ricardo Saul; Cigorraga, Selva Beatriz; et al.; Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake; Elsevier; General And Comparative Endocrinology; 179; 2; 11-2012; 254-2640016-64801095-6840enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2012.08.020info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648012003486info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:15Zoai:ri.conicet.gov.ar:11336/8470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:15.55CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
title Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
spellingShingle Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
Matzkin, Maria Eugenia
Cyclooxygenase 2
Testosterone
Fsh
Sertoli Cells
Hamsters
title_short Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
title_full Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
title_fullStr Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
title_full_unstemmed Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
title_sort Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake
dc.creator.none.fl_str_mv Matzkin, Maria Eugenia
Pellizari, Eliana Herminia
Rossi, Soledad Paola
Calandra, Ricardo Saul
Cigorraga, Selva Beatriz
Frungieri, Monica Beatriz
author Matzkin, Maria Eugenia
author_facet Matzkin, Maria Eugenia
Pellizari, Eliana Herminia
Rossi, Soledad Paola
Calandra, Ricardo Saul
Cigorraga, Selva Beatriz
Frungieri, Monica Beatriz
author_role author
author2 Pellizari, Eliana Herminia
Rossi, Soledad Paola
Calandra, Ricardo Saul
Cigorraga, Selva Beatriz
Frungieri, Monica Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cyclooxygenase 2
Testosterone
Fsh
Sertoli Cells
Hamsters
topic Cyclooxygenase 2
Testosterone
Fsh
Sertoli Cells
Hamsters
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We have previously described a stimulatory effect of testosterone on cyclooxygenase 2 (COX2) expression and prostaglandin (PG) synthesis, and the involvement of PGs in the modulation of testosterone production in Leydig cells of the seasonal breeder Syrian hamster. In this study, we investigated the existence of a COX2/PGs system in hamster Sertoli cells, its regulation by testosterone and FSH, and its effect on glucose uptake. COX2 expression was observed in Sertoli cells of both reproductively active and inactive adult hamsters. Testosterone and the plasma membrane-impermeable testosterone-BSA significantly induced COX2 expression, mitogen activated protein kinases 1/2 (MAPK1/2) phosphorylation and 15d-delta12,14PGJ2 production in Sertoli cells purified from photoperiodically regressed hamsters. These actions were abolished by the antiandrogen bicalutamide and by the inhibitor of MAPK kinase (MEK1/2) U0126, suggesting that testosterone exerts its stimulatory effect on COX2/PGs through a non-classical mechanism that involves the presence of androgen receptors and MAPK1/2 activation. FSH also stimulated COX2/PGs via MAPK1/2 phosphorylation. FSH and testosterone stimulate, whereas 15d-delta12,14PGJ2 via PPAR?× inhibits, [2,6-3H]-2-deoxy-D-glucose ([3H]-2-DOG) uptake. Meloxicam, a selective COX2 inhibitor, further increases [3H]-2-DOG uptake in the presence of FSH or testosterone. Thus, in addition to their positive effect, FSH and testosterone may also exert an indirect negative regulation on glucose uptake which involves the COX2/15d-delta12,14PGJ2/PPAR?× system. Overall, these results demonstrate the presence of a COX2/PG system in hamster Sertoli cells which might act as a local modulator of FSH and testosterone actions.
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pellizari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Rossi, Soledad Paola. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description We have previously described a stimulatory effect of testosterone on cyclooxygenase 2 (COX2) expression and prostaglandin (PG) synthesis, and the involvement of PGs in the modulation of testosterone production in Leydig cells of the seasonal breeder Syrian hamster. In this study, we investigated the existence of a COX2/PGs system in hamster Sertoli cells, its regulation by testosterone and FSH, and its effect on glucose uptake. COX2 expression was observed in Sertoli cells of both reproductively active and inactive adult hamsters. Testosterone and the plasma membrane-impermeable testosterone-BSA significantly induced COX2 expression, mitogen activated protein kinases 1/2 (MAPK1/2) phosphorylation and 15d-delta12,14PGJ2 production in Sertoli cells purified from photoperiodically regressed hamsters. These actions were abolished by the antiandrogen bicalutamide and by the inhibitor of MAPK kinase (MEK1/2) U0126, suggesting that testosterone exerts its stimulatory effect on COX2/PGs through a non-classical mechanism that involves the presence of androgen receptors and MAPK1/2 activation. FSH also stimulated COX2/PGs via MAPK1/2 phosphorylation. FSH and testosterone stimulate, whereas 15d-delta12,14PGJ2 via PPAR?× inhibits, [2,6-3H]-2-deoxy-D-glucose ([3H]-2-DOG) uptake. Meloxicam, a selective COX2 inhibitor, further increases [3H]-2-DOG uptake in the presence of FSH or testosterone. Thus, in addition to their positive effect, FSH and testosterone may also exert an indirect negative regulation on glucose uptake which involves the COX2/15d-delta12,14PGJ2/PPAR?× system. Overall, these results demonstrate the presence of a COX2/PG system in hamster Sertoli cells which might act as a local modulator of FSH and testosterone actions.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8470
Matzkin, Maria Eugenia; Pellizari, Eliana Herminia; Rossi, Soledad Paola; Calandra, Ricardo Saul; Cigorraga, Selva Beatriz; et al.; Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake; Elsevier; General And Comparative Endocrinology; 179; 2; 11-2012; 254-264
0016-6480
1095-6840
url http://hdl.handle.net/11336/8470
identifier_str_mv Matzkin, Maria Eugenia; Pellizari, Eliana Herminia; Rossi, Soledad Paola; Calandra, Ricardo Saul; Cigorraga, Selva Beatriz; et al.; Exploring the cyclooxygenase 2 (COX2)/15d delta12,14PGJ2 system in hamster Sertoli cells: regulation by FSH/testosterone and relevance to glucose uptake; Elsevier; General And Comparative Endocrinology; 179; 2; 11-2012; 254-264
0016-6480
1095-6840
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2012.08.020
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648012003486
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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