Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells

Autores
Matzkin, María E.; Gonzalez-Calvar, Silvia I.; Mayerhofer, Artur; Calandra, Ricardo S.; Frungieri, Mónica B.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters.
Instituto Multidisciplinario de Biología Celular
Materia
Ciencias Médicas
prostaglandin-endoperoxide synthase 2
prostaglandins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/82706

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/82706
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cellsMatzkin, María E.Gonzalez-Calvar, Silvia I.Mayerhofer, ArturCalandra, Ricardo S.Frungieri, Mónica B.Ciencias Médicasprostaglandin-endoperoxide synthase 2prostaglandinsWe have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters.Instituto Multidisciplinario de Biología Celular2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf163-175http://sedici.unlp.edu.ar/handle/10915/82706enginfo:eu-repo/semantics/altIdentifier/issn/1470-1626info:eu-repo/semantics/altIdentifier/doi/10.1530/rep-09-0023info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:47:51Zoai:sedici.unlp.edu.ar:10915/82706Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:47:51.98SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
spellingShingle Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
Matzkin, María E.
Ciencias Médicas
prostaglandin-endoperoxide synthase 2
prostaglandins
title_short Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_full Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_fullStr Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_full_unstemmed Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_sort Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
dc.creator.none.fl_str_mv Matzkin, María E.
Gonzalez-Calvar, Silvia I.
Mayerhofer, Artur
Calandra, Ricardo S.
Frungieri, Mónica B.
author Matzkin, María E.
author_facet Matzkin, María E.
Gonzalez-Calvar, Silvia I.
Mayerhofer, Artur
Calandra, Ricardo S.
Frungieri, Mónica B.
author_role author
author2 Gonzalez-Calvar, Silvia I.
Mayerhofer, Artur
Calandra, Ricardo S.
Frungieri, Mónica B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
prostaglandin-endoperoxide synthase 2
prostaglandins
topic Ciencias Médicas
prostaglandin-endoperoxide synthase 2
prostaglandins
dc.description.none.fl_txt_mv We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters.
Instituto Multidisciplinario de Biología Celular
description We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/82706
url http://sedici.unlp.edu.ar/handle/10915/82706
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1470-1626
info:eu-repo/semantics/altIdentifier/doi/10.1530/rep-09-0023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
163-175
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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