Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis

Autores
Guedes, Roberto M. C.; Machuca, Mariana Alejandra; Quiroga, María Alejandra; Real Pereira, Carlos E.; Resende, Talita P.; Gebhart, Connie J.
Año de publicación
2017
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection (P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104798
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/104798
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosisGuedes, Roberto M. C.Machuca, Mariana AlejandraQuiroga, María AlejandraReal Pereira, Carlos E.Resende, Talita P.Gebhart, Connie J.Ciencias VeterinariasApoptosisCaspase-3ImmunohistochemistryLawsonia intracellularisPathogenesisProliferative enteropathySwineTUNELThe purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection (P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.Facultad de Ciencias Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf620-628http://sedici.unlp.edu.ar/handle/10915/104798spainfo:eu-repo/semantics/altIdentifier/issn/1544-2217info:eu-repo/semantics/altIdentifier/doi/10.1177/0300985817698206info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:57Zoai:sedici.unlp.edu.ar:10915/104798Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:58.003SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
spellingShingle Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
Guedes, Roberto M. C.
Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
title_short Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_full Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_fullStr Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_full_unstemmed Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_sort Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
dc.creator.none.fl_str_mv Guedes, Roberto M. C.
Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
author Guedes, Roberto M. C.
author_facet Guedes, Roberto M. C.
Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
author_role author
author2 Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
topic Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
dc.description.none.fl_txt_mv The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection (P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.
Facultad de Ciencias Veterinarias
description The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection (P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104798
url http://sedici.unlp.edu.ar/handle/10915/104798
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1544-2217
info:eu-repo/semantics/altIdentifier/doi/10.1177/0300985817698206
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
620-628
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instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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