The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa
- Autores
- Aguilar, Orlando Mario; Grasso, Daniel Horacio
- Año de publicación
- 1991
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tn5-induced mutants of Rhizobium meliloti that require the amino acids isoleucine and valine for growth on minimal medium were studied. In one mutant, 1028, the defect is associated with an inability to induce nodules on alfalfa. The Tn5 mutation in 1028 is located in a chromosomal 5.5-kb EcoRI fragment.- Complementation analysis with cloned DNA indicated that 2.0 kb of DNA from the 5.5-kb EcoRI fragment restored the wild-type phenotype in the Ilv⁻ Nod⁻ mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to those for Escherichia coli DJvC and Saccharomyces cerevisiae Ilv5. Genes ilvC and ilv5 code for the enzyme acetohydroxy acid isomeroreductase (isomeroreductase), the second enzyme in the parallel pathways for the biosynthesis of isoleucine and valine. Enzymatic assays confirmed that strain 1028 was a mutant defective in isomeroreductase activity. In addition, it was shown that the ilvC genes of Rhizobium meliloti and E. coli are functionally equivalent. We demonstrated that in ilvC mutant 1028 the common nodulation genes nodABC are not activated by the inducer luteolin. E. coli ilvC complemented both defective properties (Ilv⁻ and Nod⁻) found in mutant 1028. These findings demonstrate that R. meliloti requires an active isomeroreductase enzyme for successful nodulation of alfalfa.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Rhizobium meliloti
Alfalfa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/107831
Ver los metadatos del registro completo
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The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfaAguilar, Orlando MarioGrasso, Daniel HoracioCiencias ExactasRhizobium melilotiAlfalfaTn5-induced mutants of <i>Rhizobium meliloti</i> that require the amino acids isoleucine and valine for growth on minimal medium were studied. In one mutant, 1028, the defect is associated with an inability to induce nodules on alfalfa. The Tn5 mutation in 1028 is located in a chromosomal 5.5-kb <i>Eco</i>RI fragment.- Complementation analysis with cloned DNA indicated that 2.0 kb of DNA from the 5.5-kb <i>Eco</i>RI fragment restored the wild-type phenotype in the Ilv⁻ Nod⁻ mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to those for <i>Escherichia coli</i> DJvC and <i>Saccharomyces cerevisiae</i> Ilv5. Genes <i>ilvC</i> and <i>ilv5</i> code for the enzyme acetohydroxy acid isomeroreductase (isomeroreductase), the second enzyme in the parallel pathways for the biosynthesis of isoleucine and valine. Enzymatic assays confirmed that strain 1028 was a mutant defective in isomeroreductase activity. In addition, it was shown that the <i>ilvC</i> genes of <i>Rhizobium meliloti</i> and <i>E. coli</i> are functionally equivalent. We demonstrated that in <i>ilvC</i> mutant 1028 the common nodulation genes <i>nodABC</i> are not activated by the inducer luteolin. <i>E. coli</i> <i>ilvC</i> complemented both defective properties (Ilv⁻ and Nod⁻) found in mutant 1028. These findings demonstrate that <i>R. meliloti</i> requires an active isomeroreductase enzyme for successful nodulation of alfalfa.Facultad de Ciencias Exactas1991info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf7756-7764http://sedici.unlp.edu.ar/handle/10915/107831enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC212565&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/url/https://jb.asm.org/content/173/24/7756info:eu-repo/semantics/altIdentifier/issn/0021-9193info:eu-repo/semantics/altIdentifier/pmid/1744032info:eu-repo/semantics/altIdentifier/doi/10.1128/jb.173.24.7756-7764.1991info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:56Zoai:sedici.unlp.edu.ar:10915/107831Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:56.982SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
title |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
spellingShingle |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa Aguilar, Orlando Mario Ciencias Exactas Rhizobium meliloti Alfalfa |
title_short |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
title_full |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
title_fullStr |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
title_full_unstemmed |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
title_sort |
The product of the <i>Rhizobium meliloti ilvC</i> gene is required for isoleucine and valine synthesis and nodulation of alfalfa |
dc.creator.none.fl_str_mv |
Aguilar, Orlando Mario Grasso, Daniel Horacio |
author |
Aguilar, Orlando Mario |
author_facet |
Aguilar, Orlando Mario Grasso, Daniel Horacio |
author_role |
author |
author2 |
Grasso, Daniel Horacio |
author2_role |
author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Rhizobium meliloti Alfalfa |
topic |
Ciencias Exactas Rhizobium meliloti Alfalfa |
dc.description.none.fl_txt_mv |
Tn5-induced mutants of <i>Rhizobium meliloti</i> that require the amino acids isoleucine and valine for growth on minimal medium were studied. In one mutant, 1028, the defect is associated with an inability to induce nodules on alfalfa. The Tn5 mutation in 1028 is located in a chromosomal 5.5-kb <i>Eco</i>RI fragment.- Complementation analysis with cloned DNA indicated that 2.0 kb of DNA from the 5.5-kb <i>Eco</i>RI fragment restored the wild-type phenotype in the Ilv⁻ Nod⁻ mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to those for <i>Escherichia coli</i> DJvC and <i>Saccharomyces cerevisiae</i> Ilv5. Genes <i>ilvC</i> and <i>ilv5</i> code for the enzyme acetohydroxy acid isomeroreductase (isomeroreductase), the second enzyme in the parallel pathways for the biosynthesis of isoleucine and valine. Enzymatic assays confirmed that strain 1028 was a mutant defective in isomeroreductase activity. In addition, it was shown that the <i>ilvC</i> genes of <i>Rhizobium meliloti</i> and <i>E. coli</i> are functionally equivalent. We demonstrated that in <i>ilvC</i> mutant 1028 the common nodulation genes <i>nodABC</i> are not activated by the inducer luteolin. <i>E. coli</i> <i>ilvC</i> complemented both defective properties (Ilv⁻ and Nod⁻) found in mutant 1028. These findings demonstrate that <i>R. meliloti</i> requires an active isomeroreductase enzyme for successful nodulation of alfalfa. Facultad de Ciencias Exactas |
description |
Tn5-induced mutants of <i>Rhizobium meliloti</i> that require the amino acids isoleucine and valine for growth on minimal medium were studied. In one mutant, 1028, the defect is associated with an inability to induce nodules on alfalfa. The Tn5 mutation in 1028 is located in a chromosomal 5.5-kb <i>Eco</i>RI fragment.- Complementation analysis with cloned DNA indicated that 2.0 kb of DNA from the 5.5-kb <i>Eco</i>RI fragment restored the wild-type phenotype in the Ilv⁻ Nod⁻ mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to those for <i>Escherichia coli</i> DJvC and <i>Saccharomyces cerevisiae</i> Ilv5. Genes <i>ilvC</i> and <i>ilv5</i> code for the enzyme acetohydroxy acid isomeroreductase (isomeroreductase), the second enzyme in the parallel pathways for the biosynthesis of isoleucine and valine. Enzymatic assays confirmed that strain 1028 was a mutant defective in isomeroreductase activity. In addition, it was shown that the <i>ilvC</i> genes of <i>Rhizobium meliloti</i> and <i>E. coli</i> are functionally equivalent. We demonstrated that in <i>ilvC</i> mutant 1028 the common nodulation genes <i>nodABC</i> are not activated by the inducer luteolin. <i>E. coli</i> <i>ilvC</i> complemented both defective properties (Ilv⁻ and Nod⁻) found in mutant 1028. These findings demonstrate that <i>R. meliloti</i> requires an active isomeroreductase enzyme for successful nodulation of alfalfa. |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/107831 |
url |
http://sedici.unlp.edu.ar/handle/10915/107831 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC212565&blobtype=pdf info:eu-repo/semantics/altIdentifier/url/https://jb.asm.org/content/173/24/7756 info:eu-repo/semantics/altIdentifier/issn/0021-9193 info:eu-repo/semantics/altIdentifier/pmid/1744032 info:eu-repo/semantics/altIdentifier/doi/10.1128/jb.173.24.7756-7764.1991 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 7756-7764 |
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Universidad Nacional de La Plata |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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alira@sedici.unlp.edu.ar |
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