Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes

Autores
Ventura, María Belén; Barberón, Javier Leandro; Leaden, Patricio; Zeinsteger, Pedro Adolfo; Palacios, Alejandro
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lycopene is the important pigment responsible for the characteristic red color of tomato, it has attracted attention due to its biological and physicochemical properties, especially related to its effects as a natural antioxidant. The objective of this study was to investigate the antioxidant effect of the lycopenes obtained from the extract of tomato on the peroxidation of hepatic microsomes membranes. Rat liver microsomes where incubated with different concentrations of extract (2, 4, 6, 8 μg) in an in vitro non-enzymatic ascorbic acid-Fe+2 system in order to determine the oxidative effect on membranes and to quantify peroxidation level in standardized conditions. Peroxidation was quantified in a liquid scintillation counter Packard 1900 TR by chemiluminescence in cpm (counts per minute). Microsomal membranes without extract were used as controls. Analyzing the effect of lycopene (tomato extract), was observed that the total cpm/mg protein originated from light emission: chemiluminescence, was statistically lower in samples obtained from lycopene group than in the control group (without lycopene), the antioxidant effect found was not dependent concentration.
Facultad de Ciencias Veterinarias
Materia
Veterinaria
lycopene
antioxidants
peroxidation
chemiluminescence
microsomes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/109933
Acceder
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network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver MicrosomesVentura, María BelénBarberón, Javier LeandroLeaden, PatricioZeinsteger, Pedro AdolfoPalacios, AlejandroVeterinarialycopeneantioxidantsperoxidationchemiluminescencemicrosomesLycopene is the important pigment responsible for the characteristic red color of tomato, it has attracted attention due to its biological and physicochemical properties, especially related to its effects as a natural antioxidant. The objective of this study was to investigate the antioxidant effect of the lycopenes obtained from the extract of tomato on the peroxidation of hepatic microsomes membranes. Rat liver microsomes where incubated with different concentrations of extract (2, 4, 6, 8 μg) in an in vitro non-enzymatic ascorbic acid-Fe+2 system in order to determine the oxidative effect on membranes and to quantify peroxidation level in standardized conditions. Peroxidation was quantified in a liquid scintillation counter Packard 1900 TR by chemiluminescence in cpm (counts per minute). Microsomal membranes without extract were used as controls. Analyzing the effect of lycopene (tomato extract), was observed that the total cpm/mg protein originated from light emission: chemiluminescence, was statistically lower in samples obtained from lycopene group than in the control group (without lycopene), the antioxidant effect found was not dependent concentration.Facultad de Ciencias Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf262-266http://sedici.unlp.edu.ar/handle/10915/109933enginfo:eu-repo/semantics/altIdentifier/url/https://chemrj.org/download/vol-2-iss-6-2017/chemrj-2017-02-06-262-266.pdfinfo:eu-repo/semantics/altIdentifier/issn/2455-8990info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:15:52Zoai:sedici.unlp.edu.ar:10915/109933Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:15:52.586SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
title Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
spellingShingle Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
Ventura, María Belén
Veterinaria
lycopene
antioxidants
peroxidation
chemiluminescence
microsomes
title_short Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
title_full Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
title_fullStr Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
title_full_unstemmed Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
title_sort Evaluation of Extract of Lycopene-Rich Tomatoes on the Peroxidation of Rat Liver Microsomes
dc.creator.none.fl_str_mv Ventura, María Belén
Barberón, Javier Leandro
Leaden, Patricio
Zeinsteger, Pedro Adolfo
Palacios, Alejandro
author Ventura, María Belén
author_facet Ventura, María Belén
Barberón, Javier Leandro
Leaden, Patricio
Zeinsteger, Pedro Adolfo
Palacios, Alejandro
author_role author
author2 Barberón, Javier Leandro
Leaden, Patricio
Zeinsteger, Pedro Adolfo
Palacios, Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
lycopene
antioxidants
peroxidation
chemiluminescence
microsomes
topic Veterinaria
lycopene
antioxidants
peroxidation
chemiluminescence
microsomes
dc.description.none.fl_txt_mv Lycopene is the important pigment responsible for the characteristic red color of tomato, it has attracted attention due to its biological and physicochemical properties, especially related to its effects as a natural antioxidant. The objective of this study was to investigate the antioxidant effect of the lycopenes obtained from the extract of tomato on the peroxidation of hepatic microsomes membranes. Rat liver microsomes where incubated with different concentrations of extract (2, 4, 6, 8 μg) in an in vitro non-enzymatic ascorbic acid-Fe+2 system in order to determine the oxidative effect on membranes and to quantify peroxidation level in standardized conditions. Peroxidation was quantified in a liquid scintillation counter Packard 1900 TR by chemiluminescence in cpm (counts per minute). Microsomal membranes without extract were used as controls. Analyzing the effect of lycopene (tomato extract), was observed that the total cpm/mg protein originated from light emission: chemiluminescence, was statistically lower in samples obtained from lycopene group than in the control group (without lycopene), the antioxidant effect found was not dependent concentration.
Facultad de Ciencias Veterinarias
description Lycopene is the important pigment responsible for the characteristic red color of tomato, it has attracted attention due to its biological and physicochemical properties, especially related to its effects as a natural antioxidant. The objective of this study was to investigate the antioxidant effect of the lycopenes obtained from the extract of tomato on the peroxidation of hepatic microsomes membranes. Rat liver microsomes where incubated with different concentrations of extract (2, 4, 6, 8 μg) in an in vitro non-enzymatic ascorbic acid-Fe+2 system in order to determine the oxidative effect on membranes and to quantify peroxidation level in standardized conditions. Peroxidation was quantified in a liquid scintillation counter Packard 1900 TR by chemiluminescence in cpm (counts per minute). Microsomal membranes without extract were used as controls. Analyzing the effect of lycopene (tomato extract), was observed that the total cpm/mg protein originated from light emission: chemiluminescence, was statistically lower in samples obtained from lycopene group than in the control group (without lycopene), the antioxidant effect found was not dependent concentration.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/109933
url http://sedici.unlp.edu.ar/handle/10915/109933
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://chemrj.org/download/vol-2-iss-6-2017/chemrj-2017-02-06-262-266.pdf
info:eu-repo/semantics/altIdentifier/issn/2455-8990
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
262-266
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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