Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium

Autores
Chiappe de Cingolani, Gladys Ethel; Morgan, Patricio Eduardo; Mundiña-Weilenmann, Cecilia; Casey, Joseph; Fujinaga, Jocelyne; Camilión de Hurtado, María Cristina; Cingolani, Horacio Eugenio
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The aim was to examine the regulation of the cardiac Na+-independent Cl¯/HCO3¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pHi recovery from imposed intracellular alkalinization (forward mode of exchange) and the pHi rise induced by Cl¯ removal (reverse mode). Net HCO3¯ (JHCO3¯) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial JHCO3¯ efflux after imposed alkaline load (pHi≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, P<0.05). JHCO3¯ influx induced by Cl¯ deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P<0.05) in WKY. In arbitrary units, the 4.1-kb AE1 mRNA decreased in SHR (0.15±0.01, n=7) compared to WKY (0.29±0.06, n=7, P<0.05), whereas the 3.6-kb mRNA did not change. AE2 mRNAs were similarly expressed in WKY and SHR. Cardiac specific AE3 (cAE3) mRNA decreased in SHR, 1.10±0.16 arbitrary units (n=8) versus 1.79±0.24, (n=8, P<0.05) in WKY. Full length AE3 (flAE3) mRNA increased from 0.69±0.06 (WKY, n=8) to 1.25±0.19 arbitrary units in SHR (n=8, P<0.05). Conclusions: The increase in flAE3 mRNA expression in cardiac tissue from the SHR is an adaptive change of the hypertrophied myocardium that might be in connection with the increased activity of the AE.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84718
Acceder
id SEDICI_f855aeb17ede0e502fa263c41dff5b0a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/84718
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardiumChiappe de Cingolani, Gladys EthelMorgan, Patricio EduardoMundiña-Weilenmann, CeciliaCasey, JosephFujinaga, JocelyneCamilión de Hurtado, María CristinaCingolani, Horacio EugenioCiencias MédicasGene expressionHypertensionHypertrophyIon exchangersMembrane transportObjective: The aim was to examine the regulation of the cardiac Na<SUP>+</SUP>-independent Cl¯/HCO<SUB>3</SUB>¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pH<SUB>i</SUB> recovery from imposed intracellular alkalinization (forward mode of exchange) and the pH<SUB>i</SUB> rise induced by Cl¯ removal (reverse mode). Net HCO<SUB>3</SUB>¯ (J<sub>HCO<SUB>3</SUB>¯</sub>) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial J<sub>HCO<SUB>3</SUB>¯</sub> efflux after imposed alkaline load (pH<SUB>i</SUB>≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, P<0.05). J<sub>HCO<SUB>3</SUB>¯</sub> influx induced by Cl¯ deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P<0.05) in WKY. In arbitrary units, the 4.1-kb AE1 mRNA decreased in SHR (0.15±0.01, n=7) compared to WKY (0.29±0.06, n=7, P<0.05), whereas the 3.6-kb mRNA did not change. AE2 mRNAs were similarly expressed in WKY and SHR. Cardiac specific AE3 (cAE3) mRNA decreased in SHR, 1.10±0.16 arbitrary units (n=8) versus 1.79±0.24, (n=8, P<0.05) in WKY. Full length AE3 (flAE3) mRNA increased from 0.69±0.06 (WKY, n=8) to 1.25±0.19 arbitrary units in SHR (n=8, P<0.05). Conclusions: The increase in flAE3 mRNA expression in cardiac tissue from the SHR is an adaptive change of the hypertrophied myocardium that might be in connection with the increased activity of the AE.Facultad de Ciencias MédicasCentro de Investigaciones Cardiovasculares2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf71-79http://sedici.unlp.edu.ar/handle/10915/84718enginfo:eu-repo/semantics/altIdentifier/issn/0008-6363info:eu-repo/semantics/altIdentifier/doi/10.1016/S0008-6363(01)00276-0info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:19Zoai:sedici.unlp.edu.ar:10915/84718Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:20.084SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
spellingShingle Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
Chiappe de Cingolani, Gladys Ethel
Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
title_short Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_full Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_fullStr Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_full_unstemmed Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_sort Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
dc.creator.none.fl_str_mv Chiappe de Cingolani, Gladys Ethel
Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
author Chiappe de Cingolani, Gladys Ethel
author_facet Chiappe de Cingolani, Gladys Ethel
Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
author_role author
author2 Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
topic Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
dc.description.none.fl_txt_mv Objective: The aim was to examine the regulation of the cardiac Na<SUP>+</SUP>-independent Cl¯/HCO<SUB>3</SUB>¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pH<SUB>i</SUB> recovery from imposed intracellular alkalinization (forward mode of exchange) and the pH<SUB>i</SUB> rise induced by Cl¯ removal (reverse mode). Net HCO<SUB>3</SUB>¯ (J<sub>HCO<SUB>3</SUB>¯</sub>) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial J<sub>HCO<SUB>3</SUB>¯</sub> efflux after imposed alkaline load (pH<SUB>i</SUB>≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, P<0.05). J<sub>HCO<SUB>3</SUB>¯</sub> influx induced by Cl¯ deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P<0.05) in WKY. In arbitrary units, the 4.1-kb AE1 mRNA decreased in SHR (0.15±0.01, n=7) compared to WKY (0.29±0.06, n=7, P<0.05), whereas the 3.6-kb mRNA did not change. AE2 mRNAs were similarly expressed in WKY and SHR. Cardiac specific AE3 (cAE3) mRNA decreased in SHR, 1.10±0.16 arbitrary units (n=8) versus 1.79±0.24, (n=8, P<0.05) in WKY. Full length AE3 (flAE3) mRNA increased from 0.69±0.06 (WKY, n=8) to 1.25±0.19 arbitrary units in SHR (n=8, P<0.05). Conclusions: The increase in flAE3 mRNA expression in cardiac tissue from the SHR is an adaptive change of the hypertrophied myocardium that might be in connection with the increased activity of the AE.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
description Objective: The aim was to examine the regulation of the cardiac Na<SUP>+</SUP>-independent Cl¯/HCO<SUB>3</SUB>¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pH<SUB>i</SUB> recovery from imposed intracellular alkalinization (forward mode of exchange) and the pH<SUB>i</SUB> rise induced by Cl¯ removal (reverse mode). Net HCO<SUB>3</SUB>¯ (J<sub>HCO<SUB>3</SUB>¯</sub>) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial J<sub>HCO<SUB>3</SUB>¯</sub> efflux after imposed alkaline load (pH<SUB>i</SUB>≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, P<0.05). J<sub>HCO<SUB>3</SUB>¯</sub> influx induced by Cl¯ deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P<0.05) in WKY. In arbitrary units, the 4.1-kb AE1 mRNA decreased in SHR (0.15±0.01, n=7) compared to WKY (0.29±0.06, n=7, P<0.05), whereas the 3.6-kb mRNA did not change. AE2 mRNAs were similarly expressed in WKY and SHR. Cardiac specific AE3 (cAE3) mRNA decreased in SHR, 1.10±0.16 arbitrary units (n=8) versus 1.79±0.24, (n=8, P<0.05) in WKY. Full length AE3 (flAE3) mRNA increased from 0.69±0.06 (WKY, n=8) to 1.25±0.19 arbitrary units in SHR (n=8, P<0.05). Conclusions: The increase in flAE3 mRNA expression in cardiac tissue from the SHR is an adaptive change of the hypertrophied myocardium that might be in connection with the increased activity of the AE.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84718
url http://sedici.unlp.edu.ar/handle/10915/84718
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0008-6363
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0008-6363(01)00276-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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instname:Universidad Nacional de La Plata
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collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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