HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples

Autores
Alippi, Adriana Mónica; Abrahamovich, Eliana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of Bacillus, Brevibacillus, Lysinibacillus, Paenibacillus, and Rummeliibacillus belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most Bacillus, Brevibacillus, Lysinibacillus and Paenibacillus species commonly found in honey samples facilitating isolation from polymicrobial honey.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
Materia
Ciencias Agrarias
Aerobic spore-forming bacteria
Chromogenic media
Paenibacillus
Brevibacillus
Lysinibacillus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/124318
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/124318
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samplesAlippi, Adriana MónicaAbrahamovich, ElianaCiencias AgrariasAerobic spore-forming bacteriaChromogenic mediaPaenibacillusBrevibacillusLysinibacillusThis study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey.Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124318enginfo:eu-repo/semantics/altIdentifier/issn/1879-3460info:eu-repo/semantics/altIdentifier/issn/0168-1605info:eu-repo/semantics/altIdentifier/pmid/31295678info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2019.108245info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:21:46Zoai:sedici.unlp.edu.ar:10915/124318Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:21:46.903SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
title HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
spellingShingle HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
Alippi, Adriana Mónica
Ciencias Agrarias
Aerobic spore-forming bacteria
Chromogenic media
Paenibacillus
Brevibacillus
Lysinibacillus
title_short HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
title_full HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
title_fullStr HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
title_full_unstemmed HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
title_sort HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
Abrahamovich, Eliana
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
Abrahamovich, Eliana
author_role author
author2 Abrahamovich, Eliana
author2_role author
dc.subject.none.fl_str_mv Ciencias Agrarias
Aerobic spore-forming bacteria
Chromogenic media
Paenibacillus
Brevibacillus
Lysinibacillus
topic Ciencias Agrarias
Aerobic spore-forming bacteria
Chromogenic media
Paenibacillus
Brevibacillus
Lysinibacillus
dc.description.none.fl_txt_mv This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
description This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/124318
url http://sedici.unlp.edu.ar/handle/10915/124318
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1879-3460
info:eu-repo/semantics/altIdentifier/issn/0168-1605
info:eu-repo/semantics/altIdentifier/pmid/31295678
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2019.108245
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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