HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples
- Autores
- Alippi, Adriana Mónica; Abrahamovich, Eliana
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of Bacillus, Brevibacillus, Lysinibacillus, Paenibacillus, and Rummeliibacillus belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most Bacillus, Brevibacillus, Lysinibacillus and Paenibacillus species commonly found in honey samples facilitating isolation from polymicrobial honey.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología - Materia
-
Ciencias Agrarias
Aerobic spore-forming bacteria
Chromogenic media
Paenibacillus
Brevibacillus
Lysinibacillus - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/124318
Ver los metadatos del registro completo
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HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samplesAlippi, Adriana MónicaAbrahamovich, ElianaCiencias AgrariasAerobic spore-forming bacteriaChromogenic mediaPaenibacillusBrevibacillusLysinibacillusThis study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey.Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124318enginfo:eu-repo/semantics/altIdentifier/issn/1879-3460info:eu-repo/semantics/altIdentifier/issn/0168-1605info:eu-repo/semantics/altIdentifier/pmid/31295678info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2019.108245info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:54Zoai:sedici.unlp.edu.ar:10915/124318Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:54.452SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| title |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| spellingShingle |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples Alippi, Adriana Mónica Ciencias Agrarias Aerobic spore-forming bacteria Chromogenic media Paenibacillus Brevibacillus Lysinibacillus |
| title_short |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| title_full |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| title_fullStr |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| title_full_unstemmed |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| title_sort |
HiCrome Bacillus agar for presumptive identification of <i>Bacillus</i> and related species isolated from honey samples |
| dc.creator.none.fl_str_mv |
Alippi, Adriana Mónica Abrahamovich, Eliana |
| author |
Alippi, Adriana Mónica |
| author_facet |
Alippi, Adriana Mónica Abrahamovich, Eliana |
| author_role |
author |
| author2 |
Abrahamovich, Eliana |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
Ciencias Agrarias Aerobic spore-forming bacteria Chromogenic media Paenibacillus Brevibacillus Lysinibacillus |
| topic |
Ciencias Agrarias Aerobic spore-forming bacteria Chromogenic media Paenibacillus Brevibacillus Lysinibacillus |
| dc.description.none.fl_txt_mv |
This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey. Facultad de Ciencias Agrarias y Forestales Centro de Investigaciones en Fitopatología |
| description |
This study aimed to evaluate the performance of Hicrome Bacillus™ agar for isolation and rapid identification of the aerobic spore-forming bacteria most frequently found in honey samples. A collection of 197 bacterial isolates of <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i>, <i>Paenibacillus</i>, and <i>Rummeliibacillus</i> belonging to different species that have been reported in honey were screened for their abilities to grow and for their colony colors and medium appearance in HiCrome Bacillus agar. Also, 21 strains from culture collections were used for comparison and quality controls. A flowchart utilizing a combination of colony and media characteristics in the chromogenic medium and a set of simple biochemical and morphological tests were elaborated for quick presumptive identification. A procedure for direct isolation from honey samples was developed. In conclusion, HiCrome Bacillus agar in combination with simple microbiological tests was highly useful for rapid and reliable identification of most <i>Bacillus</i>, <i>Brevibacillus</i>, <i>Lysinibacillus</i> and <i>Paenibacillus</i> species commonly found in honey samples facilitating isolation from polymicrobial honey. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-09 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/124318 |
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http://sedici.unlp.edu.ar/handle/10915/124318 |
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eng |
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eng |
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openAccess |
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