Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina

Autores
Alippi, Adriana Mónica; Fernández, Leticia A.; López, Ana Claudia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We analyzed 30 fresh bee pollen samples obtained from the main producing areas of Argentina to identify aerobic-spore-forming bacteria. We obtained 73 isolates belonging to 16 different species through isolation on selective and differential media, morphological and biochemical tests, and PCR and RFLP analysis of genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was the most predominant species (50%), followed by Bacillus megaterium (40%) and Bacillus subtilis (40%), respectively. In a minor proportion, Paenibacillus polymyxa (20%), Paenibacillus larvae (17%), Bacillus pumilus (13%), Bacillus licheniformis (13%), Bacillus amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), Bacillus coagulans (7%), Rummelliibacillus stabekisii (7%), Bacillus thuringiensis (7%), Bacillus clausii (3%), Paenibacillus alvei (3%), Bacillus simplex (3%), and Paenibacillus amylolyticus (3%) were also found. Our results showed that Argentinean bee pollen could transmit honey bee diseases due to the presence of viable spores of P. larvae and also spores of toxicogenic B. cereus s.s. and B. megaterium strains.
Facultad de Ciencias Agrarias y Forestales
Materia
Biología
Ciencias Agrarias
Bee pollen
Bacillus
Brevibacillus
Lysinibacillus
Paenibacillus
Rummeliibacillus
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131866

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spelling Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in ArgentinaAlippi, Adriana MónicaFernández, Leticia A.López, Ana ClaudiaBiologíaCiencias AgrariasBee pollenBacillusBrevibacillusLysinibacillusPaenibacillusRummeliibacillusArgentinaBee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We analyzed 30 fresh bee pollen samples obtained from the main producing areas of Argentina to identify aerobic-spore-forming bacteria. We obtained 73 isolates belonging to 16 different species through isolation on selective and differential media, morphological and biochemical tests, and PCR and RFLP analysis of genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was the most predominant species (50%), followed by Bacillus megaterium (40%) and Bacillus subtilis (40%), respectively. In a minor proportion, Paenibacillus polymyxa (20%), Paenibacillus larvae (17%), Bacillus pumilus (13%), Bacillus licheniformis (13%), Bacillus amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), Bacillus coagulans (7%), Rummelliibacillus stabekisii (7%), Bacillus thuringiensis (7%), Bacillus clausii (3%), Paenibacillus alvei (3%), Bacillus simplex (3%), and Paenibacillus amylolyticus (3%) were also found. Our results showed that Argentinean bee pollen could transmit honey bee diseases due to the presence of viable spores of P. larvae and also spores of toxicogenic B. cereus s.s. and B. megaterium strains.Facultad de Ciencias Agrarias y Forestales2021-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-8http://sedici.unlp.edu.ar/handle/10915/131866enginfo:eu-repo/semantics/altIdentifier/issn/0021-8839info:eu-repo/semantics/altIdentifier/issn/2078-6913info:eu-repo/semantics/altIdentifier/doi/10.1080/00218839.2021.1960747info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:43Zoai:sedici.unlp.edu.ar:10915/131866Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:43.33SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
title Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
spellingShingle Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
Alippi, Adriana Mónica
Biología
Ciencias Agrarias
Bee pollen
Bacillus
Brevibacillus
Lysinibacillus
Paenibacillus
Rummeliibacillus
Argentina
title_short Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
title_full Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
title_fullStr Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
title_full_unstemmed Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
title_sort Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
Fernández, Leticia A.
López, Ana Claudia
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
Fernández, Leticia A.
López, Ana Claudia
author_role author
author2 Fernández, Leticia A.
López, Ana Claudia
author2_role author
author
dc.subject.none.fl_str_mv Biología
Ciencias Agrarias
Bee pollen
Bacillus
Brevibacillus
Lysinibacillus
Paenibacillus
Rummeliibacillus
Argentina
topic Biología
Ciencias Agrarias
Bee pollen
Bacillus
Brevibacillus
Lysinibacillus
Paenibacillus
Rummeliibacillus
Argentina
dc.description.none.fl_txt_mv Bee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We analyzed 30 fresh bee pollen samples obtained from the main producing areas of Argentina to identify aerobic-spore-forming bacteria. We obtained 73 isolates belonging to 16 different species through isolation on selective and differential media, morphological and biochemical tests, and PCR and RFLP analysis of genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was the most predominant species (50%), followed by Bacillus megaterium (40%) and Bacillus subtilis (40%), respectively. In a minor proportion, Paenibacillus polymyxa (20%), Paenibacillus larvae (17%), Bacillus pumilus (13%), Bacillus licheniformis (13%), Bacillus amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), Bacillus coagulans (7%), Rummelliibacillus stabekisii (7%), Bacillus thuringiensis (7%), Bacillus clausii (3%), Paenibacillus alvei (3%), Bacillus simplex (3%), and Paenibacillus amylolyticus (3%) were also found. Our results showed that Argentinean bee pollen could transmit honey bee diseases due to the presence of viable spores of P. larvae and also spores of toxicogenic B. cereus s.s. and B. megaterium strains.
Facultad de Ciencias Agrarias y Forestales
description Bee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We analyzed 30 fresh bee pollen samples obtained from the main producing areas of Argentina to identify aerobic-spore-forming bacteria. We obtained 73 isolates belonging to 16 different species through isolation on selective and differential media, morphological and biochemical tests, and PCR and RFLP analysis of genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was the most predominant species (50%), followed by Bacillus megaterium (40%) and Bacillus subtilis (40%), respectively. In a minor proportion, Paenibacillus polymyxa (20%), Paenibacillus larvae (17%), Bacillus pumilus (13%), Bacillus licheniformis (13%), Bacillus amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), Bacillus coagulans (7%), Rummelliibacillus stabekisii (7%), Bacillus thuringiensis (7%), Bacillus clausii (3%), Paenibacillus alvei (3%), Bacillus simplex (3%), and Paenibacillus amylolyticus (3%) were also found. Our results showed that Argentinean bee pollen could transmit honey bee diseases due to the presence of viable spores of P. larvae and also spores of toxicogenic B. cereus s.s. and B. megaterium strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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http://purl.org/coar/resource_type/c_6501
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status_str publishedVersion
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dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0021-8839
info:eu-repo/semantics/altIdentifier/issn/2078-6913
info:eu-repo/semantics/altIdentifier/doi/10.1080/00218839.2021.1960747
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
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