Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey

Autores
López, Ana Claudia; Alippi, Adriana Mónica
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.
Materia
Agronomía, reproducción y protección de plantas
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/10239
Acceder
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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/10239
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honeyLópez, Ana ClaudiaAlippi, Adriana MónicaAgronomía, reproducción y protección de plantasPCR-RFLPBacillusPaenibacillusBrevibacillusLysinibacillusThis study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.Elsevier BV2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10239enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2019.105690info:eu-repo/semantics/altIdentifier/issn/0167-7012info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-10-16T09:27:14Zoai:digital.cic.gba.gob.ar:11746/10239Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-10-16 09:27:14.595CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
title Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
spellingShingle Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
López, Ana Claudia
Agronomía, reproducción y protección de plantas
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
title_short Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
title_full Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
title_fullStr Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
title_full_unstemmed Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
title_sort Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
dc.creator.none.fl_str_mv López, Ana Claudia
Alippi, Adriana Mónica
author López, Ana Claudia
author_facet López, Ana Claudia
Alippi, Adriana Mónica
author_role author
author2 Alippi, Adriana Mónica
author2_role author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
topic Agronomía, reproducción y protección de plantas
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
dc.description.none.fl_txt_mv This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.
description This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/10239
url https://digital.cic.gba.gob.ar/handle/11746/10239
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2019.105690
info:eu-repo/semantics/altIdentifier/issn/0167-7012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 12.712165

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