Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey
- Autores
- López, Ana Claudia; Alippi, Adriana Mónica
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.
- Materia
-
Agronomía, reproducción y protección de plantas
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/10239
Ver los metadatos del registro completo
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oai:digital.cic.gba.gob.ar:11746/10239 |
network_acronym_str |
CICBA |
repository_id_str |
9441 |
network_name_str |
CIC Digital (CICBA) |
spelling |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honeyLópez, Ana ClaudiaAlippi, Adriana MónicaAgronomía, reproducción y protección de plantasPCR-RFLPBacillusPaenibacillusBrevibacillusLysinibacillusThis study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.Elsevier BV2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/10239enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2019.105690info:eu-repo/semantics/altIdentifier/issn/0167-7012info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:08Zoai:digital.cic.gba.gob.ar:11746/10239Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:08.587CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
title |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
spellingShingle |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey López, Ana Claudia Agronomía, reproducción y protección de plantas PCR-RFLP Bacillus Paenibacillus Brevibacillus Lysinibacillus |
title_short |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
title_full |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
title_fullStr |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
title_full_unstemmed |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
title_sort |
Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey |
dc.creator.none.fl_str_mv |
López, Ana Claudia Alippi, Adriana Mónica |
author |
López, Ana Claudia |
author_facet |
López, Ana Claudia Alippi, Adriana Mónica |
author_role |
author |
author2 |
Alippi, Adriana Mónica |
author2_role |
author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas PCR-RFLP Bacillus Paenibacillus Brevibacillus Lysinibacillus |
topic |
Agronomía, reproducción y protección de plantas PCR-RFLP Bacillus Paenibacillus Brevibacillus Lysinibacillus |
dc.description.none.fl_txt_mv |
This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources. |
description |
This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/10239 |
url |
https://digital.cic.gba.gob.ar/handle/11746/10239 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2019.105690 info:eu-repo/semantics/altIdentifier/issn/0167-7012 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
_version_ |
1844618602800480256 |
score |
13.070432 |