Lipid binding properties of a factor necessary for linoleic acid desaturation

Autores
Leikin, Alicia Isabel; Nervi, Aníbal M.; Brenner, Rodolfo Roberto
Año de publicación
1979
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.
Facultad de Ciencias Médicas
Materia
Bioquímica
Lipid
Linoleic acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137522
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/137522
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lipid binding properties of a factor necessary for linoleic acid desaturationLeikin, Alicia IsabelNervi, Aníbal M.Brenner, Rodolfo RobertoBioquímicaLipidLinoleic acidSuspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.Facultad de Ciencias Médicas1979info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1021-1026http://sedici.unlp.edu.ar/handle/10915/137522enginfo:eu-repo/semantics/altIdentifier/issn/0024-4201info:eu-repo/semantics/altIdentifier/issn/1558-9307info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02533440info:eu-repo/semantics/altIdentifier/pmid/529999info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:24:09Zoai:sedici.unlp.edu.ar:10915/137522Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:24:09.86SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lipid binding properties of a factor necessary for linoleic acid desaturation
title Lipid binding properties of a factor necessary for linoleic acid desaturation
spellingShingle Lipid binding properties of a factor necessary for linoleic acid desaturation
Leikin, Alicia Isabel
Bioquímica
Lipid
Linoleic acid
title_short Lipid binding properties of a factor necessary for linoleic acid desaturation
title_full Lipid binding properties of a factor necessary for linoleic acid desaturation
title_fullStr Lipid binding properties of a factor necessary for linoleic acid desaturation
title_full_unstemmed Lipid binding properties of a factor necessary for linoleic acid desaturation
title_sort Lipid binding properties of a factor necessary for linoleic acid desaturation
dc.creator.none.fl_str_mv Leikin, Alicia Isabel
Nervi, Aníbal M.
Brenner, Rodolfo Roberto
author Leikin, Alicia Isabel
author_facet Leikin, Alicia Isabel
Nervi, Aníbal M.
Brenner, Rodolfo Roberto
author_role author
author2 Nervi, Aníbal M.
Brenner, Rodolfo Roberto
author2_role author
author
dc.subject.none.fl_str_mv Bioquímica
Lipid
Linoleic acid
topic Bioquímica
Lipid
Linoleic acid
dc.description.none.fl_txt_mv Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.
Facultad de Ciencias Médicas
description Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.
publishDate 1979
dc.date.none.fl_str_mv 1979
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137522
url http://sedici.unlp.edu.ar/handle/10915/137522
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0024-4201
info:eu-repo/semantics/altIdentifier/issn/1558-9307
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02533440
info:eu-repo/semantics/altIdentifier/pmid/529999
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1021-1026
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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