Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus

Autores
Wulff, Juan Pedro; Sierra, Ivana Samanta; Sterkel, Marcos; Holtof, Michiel; Van Wielendaele, Pieter; Francini, Flavio; Vanden Broeck, Jozef; Ons, Sheila
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To grow and develop insects must undergo ecdysis. During this process, the individual sheds the old cuticle to emerge as the following developmental stage. During ecdysis, different programed behaviors are regulated by neuropeptidergic pathways. In general, components of these pathways are better characterized in crustacean and holometabolous insects than in hemimetabola. In insects, the orkoninin gene produces two different neuropeptide precursors by alternative splicing: orcokinin A and orcokinin B. Although orcokinins are well conserved in insect species, their physiological role remains elusive. Here we describe a new splicing variant of the orcokinin gene in the hemimetabolous triatomine Rhodnius prolixus. We further analyze the expression pattern and the function of the alternatively spliced RhoprOK transcripts by means of immunohistochemistry and RNAi-mediated gene silencing. Our results indicate that orkoninis play an essential role in the peptidergic signaling pathway regulating ecdysis in the hemimetabolous insect Rhodnius prolixus.
Centro Regional de Estudios Genómicos
Centro de Endocrinología Experimental y Aplicada
Materia
Bioquímica
Chagas' disease
Post-embryonic development
Molting
Brain-gut neuropeptides
RNA interference (RNAi)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/128373
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixusWulff, Juan PedroSierra, Ivana SamantaSterkel, MarcosHoltof, MichielVan Wielendaele, PieterFrancini, FlavioVanden Broeck, JozefOns, SheilaBioquímicaChagas' diseasePost-embryonic developmentMoltingBrain-gut neuropeptidesRNA interference (RNAi)To grow and develop insects must undergo ecdysis. During this process, the individual sheds the old cuticle to emerge as the following developmental stage. During ecdysis, different programed behaviors are regulated by neuropeptidergic pathways. In general, components of these pathways are better characterized in crustacean and holometabolous insects than in hemimetabola. In insects, the orkoninin gene produces two different neuropeptide precursors by alternative splicing: orcokinin A and orcokinin B. Although orcokinins are well conserved in insect species, their physiological role remains elusive. Here we describe a new splicing variant of the orcokinin gene in the hemimetabolous triatomine Rhodnius prolixus. We further analyze the expression pattern and the function of the alternatively spliced RhoprOK transcripts by means of immunohistochemistry and RNAi-mediated gene silencing. Our results indicate that orkoninis play an essential role in the peptidergic signaling pathway regulating ecdysis in the hemimetabolous insect Rhodnius prolixus.Centro Regional de Estudios GenómicosCentro de Endocrinología Experimental y Aplicada2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf91-102http://sedici.unlp.edu.ar/handle/10915/128373enginfo:eu-repo/semantics/altIdentifier/issn/1879-0240info:eu-repo/semantics/altIdentifier/issn/0965-1748info:eu-repo/semantics/altIdentifier/pmid/28089691info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2017.01.003info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:08Zoai:sedici.unlp.edu.ar:10915/128373Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:08.639SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
title Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
spellingShingle Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
Wulff, Juan Pedro
Bioquímica
Chagas' disease
Post-embryonic development
Molting
Brain-gut neuropeptides
RNA interference (RNAi)
title_short Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
title_full Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
title_fullStr Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
title_full_unstemmed Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
title_sort Orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect Rhodnius prolixus
dc.creator.none.fl_str_mv Wulff, Juan Pedro
Sierra, Ivana Samanta
Sterkel, Marcos
Holtof, Michiel
Van Wielendaele, Pieter
Francini, Flavio
Vanden Broeck, Jozef
Ons, Sheila
author Wulff, Juan Pedro
author_facet Wulff, Juan Pedro
Sierra, Ivana Samanta
Sterkel, Marcos
Holtof, Michiel
Van Wielendaele, Pieter
Francini, Flavio
Vanden Broeck, Jozef
Ons, Sheila
author_role author
author2 Sierra, Ivana Samanta
Sterkel, Marcos
Holtof, Michiel
Van Wielendaele, Pieter
Francini, Flavio
Vanden Broeck, Jozef
Ons, Sheila
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Chagas' disease
Post-embryonic development
Molting
Brain-gut neuropeptides
RNA interference (RNAi)
topic Bioquímica
Chagas' disease
Post-embryonic development
Molting
Brain-gut neuropeptides
RNA interference (RNAi)
dc.description.none.fl_txt_mv To grow and develop insects must undergo ecdysis. During this process, the individual sheds the old cuticle to emerge as the following developmental stage. During ecdysis, different programed behaviors are regulated by neuropeptidergic pathways. In general, components of these pathways are better characterized in crustacean and holometabolous insects than in hemimetabola. In insects, the orkoninin gene produces two different neuropeptide precursors by alternative splicing: orcokinin A and orcokinin B. Although orcokinins are well conserved in insect species, their physiological role remains elusive. Here we describe a new splicing variant of the orcokinin gene in the hemimetabolous triatomine Rhodnius prolixus. We further analyze the expression pattern and the function of the alternatively spliced RhoprOK transcripts by means of immunohistochemistry and RNAi-mediated gene silencing. Our results indicate that orkoninis play an essential role in the peptidergic signaling pathway regulating ecdysis in the hemimetabolous insect Rhodnius prolixus.
Centro Regional de Estudios Genómicos
Centro de Endocrinología Experimental y Aplicada
description To grow and develop insects must undergo ecdysis. During this process, the individual sheds the old cuticle to emerge as the following developmental stage. During ecdysis, different programed behaviors are regulated by neuropeptidergic pathways. In general, components of these pathways are better characterized in crustacean and holometabolous insects than in hemimetabola. In insects, the orkoninin gene produces two different neuropeptide precursors by alternative splicing: orcokinin A and orcokinin B. Although orcokinins are well conserved in insect species, their physiological role remains elusive. Here we describe a new splicing variant of the orcokinin gene in the hemimetabolous triatomine Rhodnius prolixus. We further analyze the expression pattern and the function of the alternatively spliced RhoprOK transcripts by means of immunohistochemistry and RNAi-mediated gene silencing. Our results indicate that orkoninis play an essential role in the peptidergic signaling pathway regulating ecdysis in the hemimetabolous insect Rhodnius prolixus.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/128373
url http://sedici.unlp.edu.ar/handle/10915/128373
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1879-0240
info:eu-repo/semantics/altIdentifier/issn/0965-1748
info:eu-repo/semantics/altIdentifier/pmid/28089691
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2017.01.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
91-102
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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