Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses

Autores
Hernández Martínez, Salvador; Sánchez Zavaleta, Minerva; Brito, Kevin; Herrera Ortiz, Antonia; Ons, Sheila; Noriega, Fernando G.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, Anopheles albimanus and Aedes aegypti. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in Ae. aegypti mosquitoes treated with AT but not in An. albimanus, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in An. albimanus guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes in vivo in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.
Facultad de Ciencias Exactas
Centro Regional de Estudios Genómicos
Materia
Ciencias Exactas
mosquitoes
Allatotropin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/publicdomain/mark/1.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87655
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/87655
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responsesHernández Martínez, SalvadorSánchez Zavaleta, MinervaBrito, KevinHerrera Ortiz, AntoniaOns, SheilaNoriega, Fernando G.Ciencias ExactasmosquitoesAllatotropinAllatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, <i>Anopheles albimanus</i> and <i>Aedes aegypti</i>. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in <i>Ae. aegypti</i> mosquitoes treated with AT but not in <i>An. albimanus</i>, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in <i>An. albimanus</i> guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes <i>in vivo</i> in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.Facultad de Ciencias ExactasCentro Regional de Estudios Genómicos2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87655enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0175759info:eu-repo/semantics/openAccesshttp://creativecommons.org/publicdomain/mark/1.0/Creative Commons Public Domain Mark (PDM)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:14Zoai:sedici.unlp.edu.ar:10915/87655Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:15.15SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
spellingShingle Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
Hernández Martínez, Salvador
Ciencias Exactas
mosquitoes
Allatotropin
title_short Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_full Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_fullStr Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_full_unstemmed Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_sort Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
dc.creator.none.fl_str_mv Hernández Martínez, Salvador
Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author Hernández Martínez, Salvador
author_facet Hernández Martínez, Salvador
Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author_role author
author2 Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
mosquitoes
Allatotropin
topic Ciencias Exactas
mosquitoes
Allatotropin
dc.description.none.fl_txt_mv Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, <i>Anopheles albimanus</i> and <i>Aedes aegypti</i>. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in <i>Ae. aegypti</i> mosquitoes treated with AT but not in <i>An. albimanus</i>, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in <i>An. albimanus</i> guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes <i>in vivo</i> in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.
Facultad de Ciencias Exactas
Centro Regional de Estudios Genómicos
description Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, <i>Anopheles albimanus</i> and <i>Aedes aegypti</i>. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in <i>Ae. aegypti</i> mosquitoes treated with AT but not in <i>An. albimanus</i>, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in <i>An. albimanus</i> guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes <i>in vivo</i> in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87655
url http://sedici.unlp.edu.ar/handle/10915/87655
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0175759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/publicdomain/mark/1.0/
Creative Commons Public Domain Mark (PDM)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/publicdomain/mark/1.0/
Creative Commons Public Domain Mark (PDM)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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