Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses

Autores
Hernández Martínez, Salvador; Sánchez Zavaleta, Minerva; Brito, Kevin; Herrera Ortiz, Antonia; Ons, Sheila; Noriega, Fernando G.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, Anopheles albimanus and Aedes aegypti. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in Ae. aegypti mosquitoes treated with AT but not in An. albimanus, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in An. albimanus guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes in vivo in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fil: Hernández Martínez, Salvador. Instituto Nacional de Salud Pública; México
Fil: Sánchez Zavaleta, Minerva. Instituto Nacional de Salud Pública; México
Fil: Brito, Kevin. Instituto Nacional de Salud Pública; México
Fil: Herrera Ortiz, Antonia. Instituto Nacional de Salud Pública; México
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Noriega, Fernando G.. Florida International University; Estados Unidos
Materia
Aedes aegypti
Insect
Anopheles albimanus
Neuroendocrinology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49700
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responsesHernández Martínez, SalvadorSánchez Zavaleta, MinervaBrito, KevinHerrera Ortiz, AntoniaOns, SheilaNoriega, Fernando G.Aedes aegyptiInsectAnopheles albimanusNeuroendocrinologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, Anopheles albimanus and Aedes aegypti. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in Ae. aegypti mosquitoes treated with AT but not in An. albimanus, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in An. albimanus guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes in vivo in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Fil: Hernández Martínez, Salvador. Instituto Nacional de Salud Pública; MéxicoFil: Sánchez Zavaleta, Minerva. Instituto Nacional de Salud Pública; MéxicoFil: Brito, Kevin. Instituto Nacional de Salud Pública; MéxicoFil: Herrera Ortiz, Antonia. Instituto Nacional de Salud Pública; MéxicoFil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Noriega, Fernando G.. Florida International University; Estados UnidosPublic Library of Science2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49700Hernández Martínez, Salvador; Sánchez Zavaleta, Minerva; Brito, Kevin; Herrera Ortiz, Antonia; Ons, Sheila; et al.; Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses; Public Library of Science; Plos One; 12; 4; 4-2017; 1-21; e01757591932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0175759info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175759info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:18Zoai:ri.conicet.gov.ar:11336/49700instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:19.214CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
spellingShingle Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
Hernández Martínez, Salvador
Aedes aegypti
Insect
Anopheles albimanus
Neuroendocrinology
title_short Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_full Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_fullStr Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_full_unstemmed Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
title_sort Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses
dc.creator.none.fl_str_mv Hernández Martínez, Salvador
Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author Hernández Martínez, Salvador
author_facet Hernández Martínez, Salvador
Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author_role author
author2 Sánchez Zavaleta, Minerva
Brito, Kevin
Herrera Ortiz, Antonia
Ons, Sheila
Noriega, Fernando G.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Aedes aegypti
Insect
Anopheles albimanus
Neuroendocrinology
topic Aedes aegypti
Insect
Anopheles albimanus
Neuroendocrinology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, Anopheles albimanus and Aedes aegypti. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in Ae. aegypti mosquitoes treated with AT but not in An. albimanus, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in An. albimanus guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes in vivo in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fil: Hernández Martínez, Salvador. Instituto Nacional de Salud Pública; México
Fil: Sánchez Zavaleta, Minerva. Instituto Nacional de Salud Pública; México
Fil: Brito, Kevin. Instituto Nacional de Salud Pública; México
Fil: Herrera Ortiz, Antonia. Instituto Nacional de Salud Pública; México
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Noriega, Fernando G.. Florida International University; Estados Unidos
description Allatotropins (AT) are neuropeptides with pleotropic functions on a variety of insect tissues. They affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. The present work provides experimental evidence that AT elicits immune responses in two important mosquito disease vectors, Anopheles albimanus and Aedes aegypti. Hemocytes and an immune-competent mosquito cell line responded to AT by showing strong morphological changes and increasing bacterial phagocytic activity. Phenoloxidase activity in hemolymph was also increased in Ae. aegypti mosquitoes treated with AT but not in An. albimanus, suggesting differences in the AT-dependent immune activation in the two species. In addition, two important insect immune markers, nitric oxide levels and expression of antimicrobial peptide genes, were increased in An. albimanus guts after AT treatment. AT conjugated to quantum dot nanocrystals (QDots) specifically labeled hemocytes in vivo in both mosquito species, implying molecular interactions between AT and hemocytes. The results of our studies suggest a new role for AT in the modulation of the immune response in mosquitoes.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49700
Hernández Martínez, Salvador; Sánchez Zavaleta, Minerva; Brito, Kevin; Herrera Ortiz, Antonia; Ons, Sheila; et al.; Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses; Public Library of Science; Plos One; 12; 4; 4-2017; 1-21; e0175759
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49700
identifier_str_mv Hernández Martínez, Salvador; Sánchez Zavaleta, Minerva; Brito, Kevin; Herrera Ortiz, Antonia; Ons, Sheila; et al.; Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses; Public Library of Science; Plos One; 12; 4; 4-2017; 1-21; e0175759
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0175759
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613692647276544
score 13.070432

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