Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)

Autores
Saldaña, Gustavo Fabián; Martínez Alcántara, Virginia; Vinardell, José María; Bellogín, Ramón A.; Ruíz-Sainz, José E.; Balatti, Pedro Alberto
Año de publicación
2003
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isolates showed higher levels of diversity than those strains isolated from Vietnam. ARDRA analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, even though all belonged to a subcluster that included Sinorhizobium saheli and Sinorhizobium meliloti. Among S. fredii rhizobia, two strains, SMH13 and HH303, might be representatives of other species of nitrogen-fixing organisms. Although restriction analysis of the nifD–nifK intergenic DNA fragment confirmed the unique nature of Rhizobium sp. strain NGR234, several similarities between Rhizobium sp. strain NGR234 and S. fredii USDA257, the ARDRA analysis and the full sequence of the 16S rDNA confirmed that NGR234 is a S. fredii strain. In addition, ARDRA analysis and the full sequence of the 16S rDNA suggested that two strains of rhizobia might be representatives of other species of rhizobia.
Facultad de Ciencias Agrarias y Forestales
Instituto de Fisiología Vegetal
Materia
Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137359
Acceder
id SEDICI_ee28d7f28d9d164f18e4f523fcd4098c
oai_identifier_str oai:sedici.unlp.edu.ar:10915/137359
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)Saldaña, Gustavo FabiánMartínez Alcántara, VirginiaVinardell, José MaríaBellogín, Ramón A.Ruíz-Sainz, José E.Balatti, Pedro AlbertoCiencias AgrariasBiologíaSinorhizobium frediiGenotypeGenetic diversityPCRSymbiosisSoybeanThe fast-growing <i>Rhizobium</i> sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of <i>Sinorhizobium fredii</i>, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isolates showed higher levels of diversity than those strains isolated from Vietnam. ARDRA analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, even though all belonged to a subcluster that included <i>Sinorhizobium saheli</i> and <i>Sinorhizobium meliloti</i>. Among <i>S. fredii</i> rhizobia, two strains, SMH13 and HH303, might be representatives of other species of nitrogen-fixing organisms. Although restriction analysis of the <i>nifD–nifK</i> intergenic DNA fragment confirmed the unique nature of <i>Rhizobium</i> sp. strain NGR234, several similarities between <i>Rhizobium</i> sp. strain NGR234 and <i>S. fredii</i> USDA257, the ARDRA analysis and the full sequence of the 16S rDNA confirmed that NGR234 is a <i>S. fredii</i> strain. In addition, ARDRA analysis and the full sequence of the 16S rDNA suggested that two strains of rhizobia might be representatives of other species of rhizobia.Facultad de Ciencias Agrarias y ForestalesInstituto de Fisiología Vegetal2003-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf45-52http://sedici.unlp.edu.ar/handle/10915/137359spainfo:eu-repo/semantics/altIdentifier/issn/0302-8933info:eu-repo/semantics/altIdentifier/issn/1432-072Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-003-0559-yinfo:eu-repo/semantics/altIdentifier/pmid/12802480info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-12-23T11:34:27Zoai:sedici.unlp.edu.ar:10915/137359Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-12-23 11:34:27.551SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
spellingShingle Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
Saldaña, Gustavo Fabián
Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
title_short Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_full Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_fullStr Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_full_unstemmed Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_sort Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
dc.creator.none.fl_str_mv Saldaña, Gustavo Fabián
Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
author Saldaña, Gustavo Fabián
author_facet Saldaña, Gustavo Fabián
Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
author_role author
author2 Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
topic Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
dc.description.none.fl_txt_mv The fast-growing <i>Rhizobium</i> sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of <i>Sinorhizobium fredii</i>, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isolates showed higher levels of diversity than those strains isolated from Vietnam. ARDRA analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, even though all belonged to a subcluster that included <i>Sinorhizobium saheli</i> and <i>Sinorhizobium meliloti</i>. Among <i>S. fredii</i> rhizobia, two strains, SMH13 and HH303, might be representatives of other species of nitrogen-fixing organisms. Although restriction analysis of the <i>nifD–nifK</i> intergenic DNA fragment confirmed the unique nature of <i>Rhizobium</i> sp. strain NGR234, several similarities between <i>Rhizobium</i> sp. strain NGR234 and <i>S. fredii</i> USDA257, the ARDRA analysis and the full sequence of the 16S rDNA confirmed that NGR234 is a <i>S. fredii</i> strain. In addition, ARDRA analysis and the full sequence of the 16S rDNA suggested that two strains of rhizobia might be representatives of other species of rhizobia.
Facultad de Ciencias Agrarias y Forestales
Instituto de Fisiología Vegetal
description The fast-growing <i>Rhizobium</i> sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of <i>Sinorhizobium fredii</i>, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isolates showed higher levels of diversity than those strains isolated from Vietnam. ARDRA analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, even though all belonged to a subcluster that included <i>Sinorhizobium saheli</i> and <i>Sinorhizobium meliloti</i>. Among <i>S. fredii</i> rhizobia, two strains, SMH13 and HH303, might be representatives of other species of nitrogen-fixing organisms. Although restriction analysis of the <i>nifD–nifK</i> intergenic DNA fragment confirmed the unique nature of <i>Rhizobium</i> sp. strain NGR234, several similarities between <i>Rhizobium</i> sp. strain NGR234 and <i>S. fredii</i> USDA257, the ARDRA analysis and the full sequence of the 16S rDNA confirmed that NGR234 is a <i>S. fredii</i> strain. In addition, ARDRA analysis and the full sequence of the 16S rDNA suggested that two strains of rhizobia might be representatives of other species of rhizobia.
publishDate 2003
dc.date.none.fl_str_mv 2003-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137359
url http://sedici.unlp.edu.ar/handle/10915/137359
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0302-8933
info:eu-repo/semantics/altIdentifier/issn/1432-072X
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-003-0559-y
info:eu-repo/semantics/altIdentifier/pmid/12802480
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
45-52
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1852334486555983872
score 12.952241

Publicaciones similares